August | 2015 | OSU Bio Museum

Your yellow bowl is our YPT

August 31 2015September 14, 2015

Collecting insects is one of the many activities of staff and volunteers at the Triplehorn Insect Collection. Each time we go out in the field and collect we create a tiny snapshot of the insect fauna of that specific place and date. It’s far from complete, but adds to our knowledge nonetheless. We do not need to go far to find insects that no one has ever studied before. Even in our own backyard, next door to the Museum of Biological Diversity, we find new or rare insect species and discover new facts about known species.

One of the methods we frequently use to collect small flying insects is the pan trap – a bowl with water and a drop of clear, unscented liquid soap. The soap breaks the surface tension of the water and makes the insects sink. The color yellow (bright yellow!) attracts many insects, including the parasitic wasps that several of us in the collection study. Our yellow pan traps, (or YPTs for short) are simple plastic party bowls. For best results we leave the YPTs out in the field for about 24 hours. After that we remove the catch, and start the cycle again: fill the YPTs with water, add soap, leave for 24 hours, remove catch. We usually set up 25-50 YPTs in one spot and that’s one sample.

There are a couple of variations on how to empty the traps: scooping the specimens with a fine fish net, or pouring the content of the trap through the net. Either way the specimens get separated from the soapy water, and then are carefully washed with clean water to remove all the soap residue. Once washed, the specimens are preserved in 95% ethanol and placed in a freezer. That slows the degradation of their DNA and allows for molecular level studies. Later, we sort the specimens into groups (beetles, wasps, leafhoppers, etc.) and start the long process of specimen preparation for study. The YPTs are washed and saved for the next collecting season.

Click or tap on the image to enlarge.

Collecting locality across the street from the Museum.

Collecting locality next door to the Museum.

Yellow pan traps are labeled with our name & phone number.

Set pans on the ground. Avoid direct sunlight, but don’t hide them.

Add a drop of soap to each pan trap.

Add water to each pan trap.

Add water to each pan trap, don’t overfill.

Alternatively, mix soap and water before adding to the traps. Photo by H. Clebsch.

Relax and let the YPTs work for 24 hours.

Single YPT after 24 hours in the field. Awesome catch!

Check the content of the trap, remove twigs, leaves, and other debris.

Pour the content of the trap in fine fish net. Discard soapy water.

Pour (or gently scoop) the content of the trap in fine fish net. Discard soapy water.

Carefully wash the content of the yellow pan trap with clean water several times. Discard the water.

Insect specimens collected with yellow pan traps. That’s one sample.

Transfer the specimens to 95% alcohol for better preservation. Add collecting label to each sample.

Sort specimens in each sample to large groups (ex.: beetles, wasps, leafhoppers, etc.)

Sort small specimens. We need to use a microscope for that.

Wash, dry and save YPTs for next collecting season.

A heartfelt thanks to the people in these photos: Zach Hurley (collecting, adding soap, adding water, washing, sorting), Matt Elder (collecting), Norman Johnson (checking the catch, emptying YPT), Hans Clebsch (adding water to YPTs); and to the anonymous biker going by the Museum building.

About the Author: Dr. Luciana Musetti is the Curator of the Triplehorn Insect Collection at Ohio State University. All photos are courtesy of the author, except for the one of Hans Clebsch.

You’re an insect curator. Cool! So what is it you do?!

August 28 2015October 7, 2015

We frequently give tours of the Triplehorn Insect Collection to school groups, families, etc. Since most entomologists like to talk about their insects, that’s usually a fun time for me. It is also a great time to flex my General Entomology muscles. Visitors ask lots of questions, some of them interesting (How many ants are there in an ant’s nest? Do young cicadas sing underground?), some funny (Do you eat bugs every day?), some actually very difficult to answer (How many bugs are there in the world right now, how much do they weigh, and how do you know? How come you don’t have a list of all insect species in the world?).

Open collection cabinet showing insect drawers.

During a recent tour of the collection, after my brief intro on the history and structure of the collection, an 8-year old visitor asked me point blank: ‘If all the bugs in the collection are dead & put away in their little boxes, what do you do the whole day?’ Somebody behind me gasped, but I thought that was a good question, especially as I had just told them that we keep our specimens in cabinets, safe from humidity, light, and especially other bugs that might want to try and eat the dead specimens. So I told them that, yes, we keep the insects safe in cabinets, but that’s when they are not being used. And use them we do, a lot!

Much of what we know about the natural world comes from museum specimens. Scientists use the specimens and the data associated with them to answer many basic questions such as:

Where can you find this (species of) wasp? – museum specimens have labels attached to them that contain locality information. The geographic coordinates for the locality can be plotted on a map like the one below. Data for multiple specimens of a species help us understand the geographic distribution of the species.
Specimen records plotted on a map show the skewed distribution of Pelecinus polyturator, a parasitoid wasp, in North America.
When is the best time of the year to find that wasp? – specimen labels may contain a date of collection, which then may help pin-point the time of the year the species can be found in a specific place.

This label provides both detailed locality data and biological information (host species) as well.

Label including geographic coordinates, altitude, type of trap, and habitat.

With locality and date of collection alone we can already learn a tremendous amount about that species of wasp. For some areas, we can look up weather reports from that exact day and see if it was raining that day, what the average, the maximum and minimum temperatures were, also the barometric pressure, and other environmental factors that may (or may not) affect the species.

Labels may provide information on the way the specimen was collected (examples: by hand at light; using traps like a Malaise (below, middle), or yellow pans (below, right)), and the kind of vegetation (forest, grassland, prairie, etc.) encountered in the area it was collected.

Sometimes labels also include information on what the specimen was feeding on, burrowing in, coming out of, what other species it was associated with, and more. It is quite amazing how much valuable information insect collectors manage to squeeze onto a tiny piece of paper!

The label information found on museum specimens is extremely valuable data for scientists trying to learn about a species and to start assessing the impact of environmental changes on living organisms.

Now, back to the young visitor’s question: ‘What do we do the whole day?’ One of the major responsibilities of a curator (= caretaker) is to make specimens and specimen data available for scientific study. That can be done either by carefully packaging and sending the specimens on loan to a scientist, or, more recently, by databasing the specimen information and making it available online to the scientific community and the general public. Either way, that involves a lot of work, particularly in a large — we hold about 4 million dry specimens — and, relatively old — we just turned 80 in 2014 — collection like the Triplehorn. It is not uncommon for us to loan 3,000 specimens to one scientist. The largest loan we sent out to date contained 28,000 specimens – we worked on the preparation of that loan for months! We also welcome scientists who want to come on research visits to the collection.

So, just making the specimens and the specimen data available to the scientists is a lot of work. We could stop right there and we would still be very busy every day of the week. But we don’t stop there, oh no! The collection keeps growing in various ways.

Scientists deposit voucher specimens of their research with us — that’s actually one of our main missions, to house and preserve vouchers of scientific studies so other scientists can examine those specimens if and when they need to.
Private insect collectors also donate their collections to us — just last month we were presented with the late Steve Sommer Collection of butterflies and moths from Ohio and the Midwest, about 1,000 specimens.

Repairing specimens.

These new arrivals are placed in a -40°C freezer for several days to kill any potential pests that might be infesting the specimens. Next they are examined for damage, and even sometimes fixed — broken parts are glued to a card or put away into tiny vials or gel caps. After this basic upkeep work is done, we add the specimen data into our database — sounds simple, but there are many steps to that operation, and, you guessed, a lot of work involved. Finally, we add the specimens to the main collection.

I won’t get into details of the curatorial or the databasing work here, but in case you are interested, you can read about it in the collection’s blog, the Pinning Block, and follow our posts on our Facebook page.

Oh, and did I mention we collect? Yes, we collect new specimens for our own research (in my case, and Dr. Johnson’s, it is parasitoid wasps), or to fill in the gaps we have in the collection. That’s a big source of growth and (guessed righ again?) more work!

Dr. Norman Johnson collecting parasitoid wasps in South Africa.

Dr. Norman Johnson, Director of the Triplehorn Insect Collection, collecting parasitoid wasps in South Africa.

Newly collected specimens are always being prepared, dried, mounted, labeled, databased, and added to the collection. These specimens will one day be studied by scientists and maybe even be described into a new species. In the meantime it is up to the curatorial staff of the collection to keep them safe and accessible. And that’s an entirely new post. Keep tuned!

About the Author: Dr. Luciana Musetti is the Curator of the Triplehorn Insect Collection at Ohio State University. All photos are courtesy of the author.

Thanks to Norman Johnson & Gisele de Souza da Silva for careful review and thoughtful suggestions.

Welcome to the OSU Bio Museum blog

August 25 2015October 7, 2015

Today I have the pleasure to welcome you to OSU Bio Museum, a blog about biodiversity, research and museum work at the Ohio State Museum of Biological Diversity. This endeavor is the successor to our newsletter. That effort lived in both the physical and digital worlds, but to keep up with the times and changing needs, the blog is a wholly digital enterprise. The purpose remains the same, though: to share with the community the happenings, news, and successes (and sometimes failures) of the Museum. Our plan is to have weekly postings during the academic semester, with the post authors rotating among the different units in the Museum. We will also feature a Media Gallery every week. My objective in this inaugural post is to briefly describe what those units are and how the Museum is organized and functions.

The Museum, let’s call it the MBD for short, coalesced in its present form in 1992 when the University moved the bulk of the biological collections from the Columbus campus to a newly renovated building on West Campus, at our current address of 1315 Kinnear Road.

Museum of Biological Diversity on 1315 Kinnear Road

For more than 20 years the MBD has been a bit of a strange beast in that it has been a voluntary association among the collections rather than a real, defined administrative unit. Originally, most of the collections were administered by the Departments of Botany, Zoology, and Entomology. Two or three reorganizations later the primary department is Evolution, Ecology & Organismal Biology (EEOB for short) in the College of Arts & Sciences, and a smaller component associated with the Department of Entomology in the College of Food, Agriculture & Environmental Sciences (CFAES). The Entomology connection is a new one as of September 1, 2015, a reflection of a change in my formal appointment to 75% EEOB and 25% Entomology.

The overall mission of the MBD, just as the University as a whole, is teaching, research, and service. Inside the building we have, of course, the collections themselves, but also office and lab space for faculty, graduate students, postdoctoral researchers, emeriti and undergraduate students. The most glaring absence, though, is space dedicated to public exhibits. We compensate for that in two ways, our annual Museum Open House and guided tours of the facility. The tours are organized on an appointment basis only and have encompassed a wide range of groups, from elementary school kids and scout groups to University President’s Club members. Anyone interested in scheduling a guided tour of the Museum should contact us, or contact one of the collections directly to make arrangements. It’s my personal aspiration that in the future it may be possible to develop exhibit space for the public in the building, but that’s still just a gleam in my eye!

If you have not done so yet, please visit the Museum website and follow our Facebook page.

So far I’ve referred to the units of the MBD without much explanation. What are they? Well, there are seven main collections: the Triplehorn Insect Collection (which I direct, but for which Dr. Luciana Musetti is the real driving force); the Acarology Laboratory (led by Dr. Hans Klompen), the Borror Laboratory of Bioacoustics (led by Dr. Doug Nelson), the Herbarium (Dr. John Freudenstein), the Division of Molluscs (Dr. Tom Watters), the Division of Tetrapods (Ms. Stephanie Malinich), and the Division of Fishes (Dr. Meg Daly). The naming system, as I write this, must seem very confusing – what’s a collection vs. a division? The names are historical artifacts that, perhaps, made some sense at one time, but now they’re all basically equivalent. As you’ll see in my descriptions below and in future posts, there is a lot of variation among collections in their size, staffing, history and aspirations. So let’s go through the seven units:

Charles A. Triplehorn Insect Collection. The insect collection contains about 4 million prepared specimens, nearly 3,000 primary types, and one of the world’s largest leafhopper collections. The collection formally began in 1934 by Prof. Josef N. Knull, and has strong holdings in beetles (Coleoptera), Hemiptera (true bugs and hoppers), Hymenoptera (ants, bees, wasps), Odonata (dragon- and damselflies) and Orthoptera (grasshoppers and crickets). Originally the specimens largely came from the United States, but we have expanded significantly since then. Recent collecting trips have been made to Brazil, South Africa, Australia, and Malaysia (Sarawak). Ongoing research is focused on the systematics of parasitic wasps and the development of information technologies to share specimen data and images globally.

To know more about the Triplehorn collection, visit the website and follow the collection’s lively social media presence, which include the Pinning Block blog, a Facebook page, a Flickr image site & a Twitter feed.

Acarology Laboratory. Initiated by George W. Wharton in 1951, the Acarology collection is considered one of the best and most extensive insect and mite collections in North America. Over 150,000 identified and considerably more than one million unidentified specimens are included, preserved either in alcohol or on microscope slides. The geographic range is worldwide. The collection gets extensive use during the annual Acarology Summer Program, the foremost training workshop in systematic acarology in the world.

More information about the Acarology Lab can be found on their website. They also maintain the Acarology Summer Program website.

Borror Laboratory of Bioacoustics. The Borror lab is one of the leading collections of animal sound recordings in the United States. The Laboratory is named for Dr. Donald J. Borror, and entomologist and ornithologist who was a pioneer in the field of bioacoustics. He contributed many recordings including the first sound specimen in the archive, a recording of a blue jay in 1948. Today, the sound collection contains over 42,000 recordings, the majority of which are birds. Donald Borror also contributed many recordings of insects. Mammals, amphibians, reptiles, and even fish are part of the collection. The recordings are widely used for research, education, conservation, and public and commercial media.

Visit the Borror Lab website for more information and make sure to check their audio CDs.

Herbarium. The OSU Herbarium was founded in 1891 by Dr. William A. Kellerman,well-known botanical explorer of Central America, pioneer mycologist (that’s fungi!), and the University’s first professor of botany. It serves as a source of botanical data and as a base of operations for a wide variety of taxonomic, evolutionary, phytogeographical, and biochemical research programs; preserves specimens as vouchers to document present and past research studies or vegetation patters; serves as a reference point for the precise identification of plants, algae, protists, fungi and lichens; and serves the public by identifying plant specimens, providing morphological, systematic, and other information about plant species, and answering questions about plants, their properties and uses. The Herbarium currently holds over 550,000 specimens, including over 420 type specimens.

For more information about the Herbarium visit their website.

Molluscs. The Mollusc Division is really a collection of collections, containing over 1 million specimens in 140,000 lots. Over the years a number of private and institutional collections have been organized into the collection here today. The earliest large accession was that of Henry Moores (1812-1896) and was worldwide, both fossil and recent. Moores assembled one of the most diverse collections of labeled shells of that period. The University purchased this collection around 1890, added several private collections to it, and cataloged the material as part of the holdings of the first organization of the Ohio State University Museum in 1891. This collection and others were given to the Ohio State Museum on Campus in 1925, maintained and enlarged for nearly half a century, then returned to the administration of the University in 1970.

The Division of Molluscs has an interesting blog, Shell-fire and Clam-nation, and a website.

Tetrapods. The Division of Tetrapods (amphibians, reptiles, birds, and mammals) is a repository of Ohio and North American species and some worldwide research expeditions. The collections were established shortly after the founding of The Ohio State University in 1870 and grew through the collecting efforts of OSU faculty. Specimens date as far back as 1837 and include many now-protected species as well as extinct species such as the Ivory-Billed Woodpecker, Carolina Parakeet, and Passenger Pigeon. The collection houses more than 170 amphibian, 200 reptile, almost 2,000 bird and 250 mammal species.

To learn more about the OSU Tetrapod collection, visit their website and their blog, Amphibians, Reptiles, Birds and Mammals.

Fish. The Fish Division began with the collections of D. Albert Tuttle, OSU’s first zoologist. Officially recognized in 1895, the fish collection grew and moved from the Botany and Zoology Building to OSU’s Biological Station at Cedar Point, to the Ohio State Historical Society, to the Franz Theodore Stone Laboratory on Gilbraltar Island, to Sullivant Hall, and finally (whew!) to its current location as part of the Museum of Biological Diversity. The collection is primarily used as a resource for systematics research, laboratory teaching, and public education. It is also a resource for state and federal scientists who use it as a basis for comparative studies, document the geographic ranges of fish, and conduct ecological assessments and environmental impact statements.

Visit the Fish Division website for more information about their activities.

We hope you enjoy the blog and please send us your feedback!

About the Author: Dr. Norman F. Johnson is a Professor with appointments in the Department of Evolution, Ecology and Organismal Biology & the Department of Entomology at The Ohio State University. He is also the Director of the Triplehorn Insect Collection. Norman studies the systematics and evolution of parasitoid wasps in the family Platygastridae (Hymenoptera).

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Month: August 2015

Your yellow bowl is our YPT

You’re an insect curator. Cool! So what is it you do?!

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