Collecting the small plants

 

When told that a herbarium is a collection of plants, most people think of flowering plants or pine trees, or perhaps even ferns. The herbarium possesses these plants, but it also has other plants – an often, overlooked group of plants, the bryophytes that include mosses, liverworts and hornworts.

An example of a bryophyte, the ribbed bog moss, Aulacomnium palustre, with stalks of propagules that will be dispersed for asexual reproduction. From a wet meadow at Waldo, Marion County, Ohio. April 21, 2006. Photo by Bob Klips.

An example of a bryophyte, the ribbed bog moss, Aulacomnium palustre, with stalks of propagules that will be dispersed for asexual reproduction. From a wet meadow at Waldo, Marion County, Ohio. April 21, 2006. Photo by Bob Klips.

Bryophytes are small. As a result, the characters that distinguish bryophytes are small, microscopically so, but the array of beauty and intricacy displayed in flowering plants also are present in bryophytes. Those researchers that study bryophytes, bryologists, are privileged to observe this vibrant world of miniature plants.

An example of the complexity and elegance of the spore-producing structures of the small-mouthed thread moss, Bryum lisae var. cuspidatum, as observed by a bryologist. Alum Creek State Park, Waldo, Marion County. April 17, 2008. Photo by Bob Klips.

An example of the complexity and elegance of the spore-producing structures of the small-mouthed thread moss, Bryum lisae var. cuspidatum, as observed by a bryologist. Alum Creek State Park, Waldo, Marion County. April 17, 2008. Photo by Bob Klips.

 

Bryophytes are small plants and often require the use of dissecting and compound microscopes to view diagnostic characters. Here, bryologist, Diane Lucas, uses the compound microscope to view the shape and size of the leaf cells of a moss.

Bryophytes are small plants and often require the use of dissecting and compound microscopes to view diagnostic characters. Here bryologist Diane Lucas uses the compound microscope to view the shape and size of the leaf cells of a moss.

A leaf of the moss, Bryum flaccidum, showing hexagonal leaf cells. Moss and liverwort leaves are only one cell layer thick, thus each individual leaf cell is easily visible, as seen here viewed with the compound microscope. The shape and size of the leaf cells are often used to distinguish moss species.

A leaf of the moss, Bryum flaccidum, showing hexagonal leaf cells. Moss and liverwort leaves are only one cell layer thick, thus each individual leaf cell is easily visible, as seen here viewed with the compound microscope. The shape and size of the leaf cells are often used to distinguish moss species.

Bryophytes often grow in places where other plants cannot grow, such as on the sides of trees or on the surface of boulders. Bryophytes are able to grow on such substrates because they are able to survive after drying to conditions equal to the water content of the surrounding environment, conditions that would cause wilting and death in other plants. Poikilohydry, this ability to dry and then re-establish growth in the presence of moisture, is a character that flowering plants have evolutionarily lost. In herbaria, the poikilohydric nature of bryophytes has been observed in some specimens that are able to grow after five, ten or twenty years dried in a herbarium.

A  typical habitat of the rounded tongue moss, Anomodon minor, on limestone rock. From Duranceaux Park, Delaware County, Ohio. April 24, 2011. Photo by Bob Klips.

A typical habitat of the rounded tongue moss, Anomodon minor, on limestone rock. From Duranceaux Park, Delaware County, Ohio. April 24, 2011. Photo by Bob Klips.

Bryophyte specimens are easier to collect and to preserve compared to other plants because they do not require pressing, or mounting onto herbarium sheets. While in the field, bryophyte plants are assigned a collection number and placed into small paper bags or paper envelopes, where they are dried. In the herbarium, bryophytes are stored in envelope packets that are made from 100% cotton rag archival paper. Labels with species identification, collection location, habitat information, collection date and collector are printed onto the face of the envelope. The envelopes are stored in flat boxes specially designed to fit on the shelves of herbarium cabinets.

Bryophytes are collected in the field in paper bags or envelopes. The bag in the photo has a collection number at the top, followed by a tentative field identification and the substrate on which the moss (shown on top of bag) was collected.

Bryophytes are collected in the field in paper bags or envelopes. The bag in the photo has a collection number at the top, followed by a tentative field identification and the substrate on which the moss (shown on top of bag) was collected.

Typical information on face of a bryophyte packet, in this case, a packet of a moss from Crawford County, Ohio.

Typical information on face of a bryophyte packet, in this case, a packet of a moss from Crawford County, Ohio.

An open packet showing moss plants stored inside.

An open packet showing moss plants stored inside.

Flat boxes store bryophyte packets inside herbarium cases.

Flat boxes store bryophyte packets inside herbarium cases.

A herbarium case with two rows of boxes that contain packets of bryophyte specimens.

A herbarium case with two rows of boxes that contain packets of bryophyte specimens.

The Ohio State University Herbarium contains over 10,000 specimens of bryophytes – a bryologist’s delight.

From a bryologist's point of view -  delighting in the world of small plants: the moss, Fissidens subbasilaris, with stalks subtended by oblong sporangia that contain spores. From Christmas Rocks State Nature Preserve, Fairfield County, Ohio. September 7, 2014. Photo by Bob Klips.

From a bryologist’s point of view – delighting in the world of small plants: the moss, Fissidens subbasilaris, with stalks subtended by oblong sporangia that contain spores. From Christmas Rocks State Nature Preserve, Fairfield County, Ohio. September 7, 2014. Photo by Bob Klips.

 

 

About the Author: Dr. Cynthia Dassler is Curator of Cryptogams (small plants that produce spores) at The Ohio State Herbarium (OS) in the Department of Evolution, Ecology and Organismal Biology.

Your yellow bowl is our YPT

 

Collecting insects is one of the many activities of staff and volunteers at the Triplehorn Insect Collection. Each time we go out in the field and collect we create a tiny snapshot of the insect fauna of that specific place and date.  It’s far from complete, but adds to our knowledge nonetheless. We do not need to go far to find insects that no one has ever studied before. Even in our own backyard, next door to the Museum of Biological Diversity, we find new or rare insect species and discover new facts about known species.

One of the methods we frequently use to collect small flying insects is the pan trap – a bowl with water and a drop of clear, unscented liquid soap.  The soap breaks the surface tension of the water and makes the insects sink.  The color yellow (bright yellow!) attracts many insects, including the parasitic wasps that several of us in the collection study.  Our yellow pan traps, (or YPTs for short) are simple plastic party bowls.  For best results we leave the YPTs out in the field for about 24 hours.  After that we remove the catch, and start the cycle again: fill the YPTs with water, add soap, leave for 24 hours, remove catch. We usually set up 25-50 YPTs in one spot and that’s one sample.

There are a couple of variations on how to empty the traps: scooping the specimens with a fine fish net, or pouring the content of the trap through the net. Either way the specimens get separated from the soapy water, and then are carefully washed with clean water to remove all the soap residue. Once washed, the specimens are preserved in 95% ethanol and placed in a freezer.  That slows the degradation of their DNA and allows for molecular level studies.  Later, we sort the specimens into groups (beetles, wasps, leafhoppers, etc.) and start the long process of specimen preparation for study.  The YPTs are washed and saved for the next collecting season.

Click or tap on the image to enlarge.

 

A heartfelt thanks to the people in these photos: Zach Hurley (collecting, adding soap, adding water, washing, sorting), Matt Elder (collecting), Norman Johnson (checking the catch, emptying YPT), Hans Clebsch (adding water to YPTs); and to the anonymous biker going by the Museum building.

 

About the Author: Dr. Luciana Musetti is the Curator of the Triplehorn Insect Collection at Ohio State University. All photos are courtesy of the author, except for the one of Hans Clebsch.