High School Outreach

“Ocean viruses: From isolates to genomes” — A laboratory course

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Presentations and protocols for download

1. Introduction: Ocean Viruses (pptx)
2. Project Overview (doc)
3. Isolation of cyanophages
   1. Protocol: Plating (doc)
   2. Placque Picking(pptx)
   3. Protocol: Placque Picking (doc)
   4. Protocol: DNA Extraction (doc)
   5. Genome assembly (pptx)
   6. Protocol: Genome assembly (doc)
   7. Protocol: Genome annotation (doc)
4. Viral Genomics (pptx)
   1. PCR (pptx)
   2. Protocol: PCR 1(pdf)
   3. Gel electrophoresis (pptx)
   4. Protocol: PCR gel 1 (pdf)
   5. Gel Interpretation (pptx)
   6. Protocol: PCR 2 (pdf)Protocol: PCR gel 2 (pdf)
   7. DNA Cleanup (pptx)
   8. Protocol: MinElute (pdf)
5. TEM (pdf)