April 5 @12:30pm

Dr. John Sausen, Agilent Technologies, Director of Strategic Initiatives — Mass Spectrometry

“Advancing Ion Mobility into Lipidomics, Metabolomics, Exposomics, and Protein Characterization”

Date: Friday, April 5, 2024
Time: 12:30pm
Location: 014 University Hall

Abstract
Laboratories conducting Multi-Omics analysis have challenging requirements to better define isomeric and isoberic structures across a broad mass range in a relatively short time scale – increasing throughput and reducing cost per sample. This applies to Lipidomics, Metabolomics and Proteomics as well as Protein characterization. Specifically with Native Protein Structures, it would be helpful to determine if there are multiple proteoforms present in putative proteomics biomarkers and therapeutic biomolecules. Recent advancements in Ion Mobility are making this possible. Multiplexing improves Sensitivity, dynamic range, extends the mass range to lower mass. It also enables added statistical significance enabling High Resolution Demultiplexing which can dramatically increase Drift Resolution of Isomers and Isobars while maintaining fast UHPLC acquisition rates as well as a wide mass range – single injection with both high drift resolution and excellent chromatographic fidelity. In addition, Ion Mobility can be predictive in assisting with identifications of unknowns not found in a data base. For example, leveraging high precision CCS Collisional Cross Sections of a homologous series of Lipids or PFAS subunits, or enabling Machine Learning to better predict which unknown isomer hypothesis has the most likelihood of being accurate. The better the precision in the training set, the more accurate the prediction of the putative unknown isomer – will show PFAS as an example, could apply to many isomeric compound classes. We can now achieve and leverage precision of the CCS (0.4 – 0.2%RSD – great precision in the CCS) enabling better accuracy in the predictions. Another important subject is Protein Characterization. Several techniques and assist in helping to determine if there are multiple protoeforms present – Collision Induced Unfolding, being one – compare profiles of a putative protein marker in disease Vs not diseased, or a therapeutic in a bioreactor as one optimizes changing feedstocks – quickly determine if something changed. Agilent has improved the precision to both the CCS and CIU50 Voltage values of native protein unfolding, enabling more confidence in rapidly determining if the protein structure has changed. In addition, with newer electrodes used in the CIU experiments, we are now able to work with larger native protein structures, as large as GroEL. Realtime Microdroplet flash characterization can also offer interesting tools to better rapidly characterize higher order structures.

Bio
John has been a part of Agilent Technologies and Hewlett Packard for the past 41 years. He is currently Director of Strategic Initiatives – Mass spectrometry, supporting the America’s field organization. He works with thought leaders in a a business development capacity, building collaborations and collecting customer feedback on changing requirements in the life science markets, with a focus toward triple quad, QTOF, and Ion  Mobility technology and applications.

 

February 23 @12:30pm

Ms. Kristie Baker, Wysocki Group, Ohio State
“Characterizing a Surface Bridge in the Four-Helix Bundle Rop by Surface- and Collision-Induced Activation”

and

Philip Lacey, Wysocki Group, Ohio State
“Heterogeneous Non-Canonical Inflammasome Oligomerization Probed by Narrow Quadrupole Selections and Electron Capture Charge Reduction”

Date: Friday, February 23, 2024
Time: 12:30pm
Location: 0035 Hitchcock Hall

January 26 @12:30pm

Mr. Alex Grooms, Badu Group, Ohio State
“Uncatalyzed Hydrazine Cross Coupling via Plasma-Microdroplet Fusion Enables Programmable C–N Bond Formation”

Date: January 26, 2024
Time: 12:30 PM
Location 0035 Hitchcock Hall

December 12 @5:30pm

Ms. Ayesha Seth, Badu Group, Ohio State
“Revolutionizing Malaria Detection: Pioneering Chemical Signal Amplification for Asymptomatic Cases with Mass Spectrometry”

and

Mr. Joseph Holbrook, Hummon Group, Ohio State
“Enhancement of Lipid Signals in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry with Ammonium Fluoride as a Matrix Additive”

Date: Dec 12, 2023
Time: 5:30 PM
Location: BRT 105

Meeting — November 14 @5:30pm

Ms. Ariana Shannon (Hummon/Searle Group)

“Generating fit-for-purpose targeted mass-spectrometry based proteomics assays from a global proteome catalog”

Date: Nov 14, 2023 (TODAY)
Time: 5:30 PM
Location: BRT 105

Meeting — October 24 @5:30pm

Dr. Joshua Gilbert, Wysocki Research Group, Ohio State

“Combining Soft-Landing MS and Low-Energy Electron Holography for Structural Biology”

Date: October 24, 2023
Time: 5:30 PM
Location: BRT 105

Meeting — September 19 @5:30pm

Mr. Alex Grooms, Badu Lab, Ohio State
“Programmable Microdroplet Cascade Reactions: Uncatalyzed Michael Addition for Carbon-Carbon Bond Formation via Plasma-Water Fusion”

and

Mr. Daniel Quiroz Moreno (Cooperstone Group)
“Lessons Learned from Data Dependent Acquisition and Molecular Networking: Case Application in Phenolics”

Date: September 19, 2023
Time: 5:30 PM
Location: BRT 105

Meeting — April 18 @5:30pm

Mr. Alex Grooms, Badu Lab, Ohio State
“Dual Tunability for Uncatalyzed N-alkylation of Primary Amines Enabled by Super Reactive Electrospray Droplets”

and

Mr. Brian Fries, Hummon Lab, Ohio State
“Fatty Acid Synthase Inhibition in Colorectal Cancer Spheroids”

Date: April 18, 2023
Time: 5:30 PM
Location: DHLRI 170

Meeting — March 28 @5:30pm

Dr. Marius Kostelic, Wysocki Lab, Ohio State
“Architecture of Adeno-Associated Viral Capsids with Surface-Induced Dissociation and Charge Detection Mass Spectrometry”

and

Mr. Daniel Quiroz Moreno, Cooperstone Lab, Ohio State
“MS2extract: An R Package for a Scalable MS/MS Compound Library Creation”

Date: March 28, 2023
Time: 5:30 PM EST
Location: DHLRI 170

Meeting — February 21 @5:30pm

Zihao Qi, Wysocki Lab, Ohio State, “Native Mass Spectrometry of Single Strand Annealing Protein and Nucleoprotein Complexes”

and

Enoch Amoah, Badu Lab, Ohio State, “Mass Spectrometry Approach for Differentiation of Positional Isomers of Saccharides: Toward Direct Analysis of Rare Sugars”

Date: February 21, 2022
Time: 5:30 PM Eastern
Location: DHLRI 170

Meeting — December 13 @5:30pm

Emily Sekera, Hummon Lab, Ohio State. “Mass Spectrometry Imaging of 3D Cell Cultures: From Drug Treatments to Dietary Changes”

Date: December 13, 2022
Time: 5:30 PM
Location: BRT 115

Meeting — November 15 @5:30pm

Dr. Andrew Arslanian, Wysocki Group, Ohio State. “Gas Phase Techniques for the Study of Biomolecular and Supramolecular Structures and Chemistry”

Date: November 15, 2022
Time: 5:30 PM Eastern
Location: CBEC 267

Meeting — October 3rd @5:30pm

Prof. David Russell, Texas A&M University, Advanced MS Technologies for Studies for Biomolecule Complexes: Structure, Stability and Dynamics”

Date: October 3, 2022
Time: 5:30 PM EST
Location: CBEC 130

Meeting – Sept 20th @ 5:30pm

Mr. Alex Grooms, Badu Lab, Ohio State, “Plasma-Droplet Reaction Systems: A Direct Mass Spectrometry Approach for Enhanced Characterization of Lipids at Multiple Isomer Levels”

Date: September 20, 2022
Time: 5:30 PM EST
Location: CBEC 267

Virtual Analytical Seminar – October 25th @ 4:10pm

Title: “Exhalomics – on-line medical diagnosis via mass spectrometric analysis of exhaled breath”

On-line breath analysis is a powerful approach to obtain insight into the metabolism of a person in real time. With ambient ionization methods, this can be achieved rapidly and completely non-invasively, opening up interesting possibilities to diagnose diseases via exhaled breath, to discern diurnal changes in the metabolism, and to monitor drug use (or drug abuse) by analyzing the chemical composition of exhaled breath.

…. Read More

Date: October 25th, 2021
Time: 4:10pm
Zoom: To be provided

Virtual Meeting – Sept 14th @ 5:30pm

Mr. Taghi Sahraeian, Badu Research Group “Droplet Imbibition Mass Spectrometry”

Date: Septh 14th, 2021
Time: 5:30 PM EST
Zoom: link provided via email or contact badu-tawiah.1@osu.edu

Virtual Analytical Seminar – August 30th @ 4:10pm

Title: “Fishing out elusive species from chemical reactions”

Prof. Shibdas Banerjee will be giving a talk at CBC’s analytical/physical seminar series. Shibdas is currently an Assistant Professor at Indian Institute of Science Education and Research Tirupati. He recently completed his postdoctoral studies with Prof. Dick Zare at Stanford University.

Date: August 30th, 2021
Time: 4:10 PM EST
Zoom: https://osu.zoom.us/j/94054048207?pwd=TUVHSDRvV3F6dGI3S3JSSE1aREhoZz09 

Virtual Meeting – March 23rd @ 5:30pm

Prof. Justin Benesch, University of Oxford “Combining native mass spectrometry with mass photometry to elucidate the assembly of ultra-heterogeneous protein assemblies”

Date: March 23rd, 2021
Time: 5:30 PM EST
Zoom: link provided via email or contact badu-tawiah.1@osu.edu

Virtual Meeting – February 23rd @ 5:30pm

Dr. Candice Ulmer, Research Chemist, CDC “The Development of Routine and Reference Measurement Procedures to Support the Accurate Reporting of Clinical Measurements”

Date: February 23rd, 2021
Time: 5:30 PM EST
Zoom: link provided via email or contact badu-tawiah.1@osu.edu

Virtual Meeting – Jan 26th @ 5:30pm

Dr. Michael Wleklinski, Senior Scientist, Merck & Co. “High Throughput Synthesis and Screening: My Journey from Academia to Industry”

Date: December 8, 2021
Time: 5:30 PM EST
Zoom: link provided via email or contact badu-tawiah.1@osu.edu

Virtual Meeting – Dec 8th @ 5:30pm

Prof. Julia Laskin, Purdue University “Ambient imaging of biological samples using nanospray desorption electrospray ionization (nano-DESI) mass spectrometry”

Date: December 8, 2020
Time: 5:30 PM EST
Zoom: link provided via email or contact badu-tawiah.1@osu.edu

Virtual Meeting – Nov 17th @ 5:30pm

1. Dr. Maya Reimi Sipala, Scientist at Balazs NanoAnalysis, “HR-ICP-MS Applications in the Semiconductor Industry”

Date: November 17, 2020
Time: 5:30 PM EST
Zoom: to be provided via email

Special CBC Analytical Chemistry Seminar, Nov 2nd @ 4:10pm

Prof. Peter Nemes, University of Maryland, “Single-cell Mass Spectrometry Discovers Metabolic Effectors of Cell Differentiation”

Date: November 2, 2020
Time: 4:10 PM EST
Place: Zoom – link provided via email or contact badu-tawiah.1@osu.edu

Virtual Meeting – Oct 13th @ 5:30pm

1. Prof. Carlito Lebrilla, Department of Chemistry, UC Davis “Carbohydrates as biomarkers for health and diseases”

Date: October 13, 2020
Time: 5:30 PM (EST)
Zoom: to be provided

Virtual Meeting – Sept 15th @ 5:30pm

1. Dr. Mike Freitas
“Moving mass Spectrometry informatics into the cloud. A case study report on the implementation of AWS cloud based workflows for mass spectrometry data analysis.”

Date: Sept 15th
Time: 5:30pm
Place: Zoom – link to be provided via email

COVID-19 Notice

In keeping with directions provided by the University in regards to corona virus pandemic, MSDG meetings will be suspended for the remainder of the semester.

All speakers have been notified of this change

Meeting – Feb 24th @ 5:30pm

1. Prof. Jim Prell, University of Oregon
“Quantitative Protein Unfolding with a Q-TOF”

Date: February 24th
Time: 5:30 PM
Place: 105 BRT 

Meeting – Jan 21st @ 5:30pm

1. Dr. Ralph Tobias, Hummon Group
“Developing a Drug Screening Platform: MALDI-Mass Spectrometry Imaging of Paper-Based Cultures”

2. Ms. Haley Chatelaine, Kopec Group
“A Targeted and Untargeted LC-MS Approach to Determining Postprandial Vitamin A&D and Lipid Levels in Metabolic Syndrome versus Healthy Subjects”

Date: January 21st
Time: 5:30 PM
Place: 105 BRT

Meeting – Nov 26th @ 5:30pm

1. Dr. Djawed Bennouna, Kopec Research Group, OSU
“The Effects of Omega-3 Supplementation on the Brain Lipids after Chemotherapy”

2. Dr. Dmytro Kulyk, Badu Research Group, OSU
“High-Throughput Mass Spectrometry Platform for Gas-Phase Reaction Screening under Ambient Conditions”

Date: November 26
Time: 5:30 PM
Place: 105 BRT

Seminars – Oct 22th 5pm – “Science is Too Important to be Left Just to Men”

There will be a special seminar next week by Dr. Debra Rolison from the Naval Research Laboratory.

Title: “Science is Too Important to be Left Just to Men”
Place: E0040
Date: Oct 22, 2019
Time: 5 – 6 pm

Since it coincides with our October meeting, MSDG is canceled for this month.

This seminar will be beneficial for grad students/postdocs and we encourage them to attend.

Dr. Rolison will give research presentation earlier in the day.

Title: “Scalable Multifunctional Nanoarchitectures for Energy Storage”

Oct 22, 11:30 AM, 100 Stillman

Bio: https://en.wikipedia.org/wiki/Debra_R._Rolison

Meeting – Sept 24th @ 5:30PM

Topic: Thermal ionization mass spectrometry methods and applications in the earth and ocean sciences

Overview by Prof. Elizabeth Griffith, School of Earth Sciences, OSU

Application by Ms. Samantha Carter, Griffith Research Group, OSU

Date: September 24
Time: 5:30 PM
Place: JAMES B050 (460 W. 10TH Ave)

Don’t miss OMSMS 2019!

Please join us for the 3rd Annual Ohio Mass Spectrometry and Metabolomics Symposium (OMSMS) to be held at the Blackwell Inn and Pfahl Conference Center at The Ohio State University in Columbus, Ohio, on October 1-2, 2019.

  • Invited keynote and plenary presentations:
    • Connecting the World’s Mass Spectrometry Data to Understand the Chemistry of Life — a Big Data Strategy, Pieter Dorrestein, UCSD
    • Mass Spectrometry Imaging Applications to Support Clinical Decision Making, Nathalie Agar, Harvard Medical School
    • Harnessing Metabolomics to Combat Infectious Diseases, Ian Lewis, University of Calgary
    • The Exposome Meets Precision Nutrition, Susan Sumner, UNC
  • Introductory workshops in Metabolomics and Native Mass Spectrometry
  • Oral, poster and flash talk presentations (submitted abstracts)
  • Evening reception
  • Lunch and Learn sessions
  • Networking opportunities

REGISTER TODAY! Registration closes September 17

The purpose of the two-day joint meeting of the 16th Annual Ohio Mass Spectrometry Symposium and 3rd Annual Conference on Food and Nutritional Metabolomics is to provide an opportunity for academic and industrial researchers in the region to present their findings, share information, discuss research challenges with colleagues, and spark new collaborations in the rapidly advancing fields of mass spectrometry and metabolomics. Key topics will include Advanced Ionization Techniques and Instrumentation, Structural Biology, and Personalized Nutrition.

The symposium is planned and co-hosted by the OSU Campus Chemical Instrument Center, National Resource for Native MS-Guided Structural Biology, and the OSU Foods for Health program.

OMSMS is made possible with generous sponsorships from Center for Applied Plant Sciences (CAPS), Metabolon, Abbott, Agilent, Bruker, SCIEX, Waters, Thermo Fisher Scientific, 908 Devices, Advion, and Biocrates.

Visit go.osu.edu/omsms for additional symposium information and links to registration.

Meeting – August 27th @ 5:30PM

Mr. Taghi Sahraeian, Badu Research Group, OSU
“Droplet Imbibition Enables Non-Equilibrium Interfacial Reactions in Charged Microdroplets”

Date: August 27th
Time: 5:30 PM
Place: 105 BRT

COH-MSDG – Central Ohio (OH) Mass Spectrometry Discussion Group

Seminar – August 20th 4:00PM – “Deciphering Proteins with Unknown Functions in Plants using Top-Down and Native Mass Spectrometry Techniques”

Mowei Zhou will present a seminar while visiting us from Pacific Northwest National Laboratory next week:

“Deciphering Proteins with Unknown Functions in Plants using Top-Down and Native Mass Spectrometry Techniques”

Tuesday, August 20, 2019
4:00 pm
BRT 105

Mass spectrometry (MS) has been widely used in proteomics applications for identifying and quantifying proteins in biological samples, particularly in the field of biomedical research. In recent years, many new MS techniques, including top-down MS, and native MS for comprehensive characterization of post-translational modification (PTM), ligand/metal binding, higher-order structures, and protein-protein interactions. At the Environmental Molecular Sciences Laboratory (EMSL), a Department of Energy (DOE) user facility located at the Pacific Northwest National Laboratory (PNNL), we aim to use advanced analytical tools to build up a mechanistic understanding of biological processes that affect the environment, and to harness enzymes that can produce sustainable “green” fuels from biomass. Despite the fast-expanding knowledge of plant and microbial organisms from genomics and transcriptomics research, many of the genes still have unknown functions and remain to be characterized at the protein level. I will highlight a few recent EMSL projects applying top-down and native MS to uncharacterized proteins. The focus will be on histone modifications in epigenetic control of drought stress in sorghum crop; a plant enzyme heterocomplex in vitamin B6 biosynthesis with tunable stoichiometry; and an unknown plant enzyme complex controlling lignin synthesis.

Seminar – June 25th 10:00-11:00am “Uncovering Antimicrobial Peptide Interactions in Lipid Nanodiscs by Native MS”

A special seminar will be presented by Dr. Michael T. Marty (University of Arizona) on
Uncovering Antimicrobial Peptide Interactions in Lipid Nanodiscs by Native MS.

Tuesday, June 25th
10:00-11:00 am
BRT 105

Research in the Marty lab is focused on developing mass spectrometry methods to study the structure and biophysics of membrane proteins. Working at the interface of Analytical Chemistry and Biochemistry, they utilize lipoprotein Nanodiscs to solubilize membrane proteins in a lipid bilayer with a defined composition. Dr. Marty is at the forefront of development of new computational approaches and software for analysis of native MS data. This work builds on UniDec software developed to rapidly and robustly deconvolve mass and ion mobility spectra.

The seminar is supported by the Resource for Native MS-Guided Structural Biology.

Seminiar – May 22 1:30-2:30pm “The importance of isotopic fine structures in ultrahigh resolution FT-ICR analyses”

We will have an off-semester seminar presented by Prof. Eugene Nikolaev (Skoltech Institute of Science and Technology, Moscow, Russia) on
“The importance of isotopic fine structures in ultrahigh resolution FT-ICR analyses”.

Prof. Nikolaev is a well-known and prominent scientist who has made significant contribution to Fourier-transform Ion Cyclotron Resonance (FT-ICR) mass spectrometry analyses and instrument design. We allocated some extra time for discussion after his seminar.
I hope to see you many of you at Prof. Nikolaev’s seminar.

Time: May 22nd (Wed) 1:30-2:30 pm
Location: DHLRI 165

Meeting – April 23rd @ 5:30pm

1. Mr. Ben Burris, Badu Research Group, OSU
“Probing Kinetics of Biomolecular Reactions via Contained-ESI Mass Spectrometry”

2. Ms. Maddy Lomax-Vogt, Olesik Research Group, OSU
“Inductively Coupled Plasma Fundamentals and Nanoparticle Measurements”

Date: April 23
Time: 5:30 PM
Place: 2015 MP

Meeting – March 25th @ 5:30pm

Prof. Jenny Brodbelt, Department of Chemistry, The University of Texas at Austin
“Lipid analysis using UVPD”

Date: March 25
Time: 5:30 PM
Place: BRT 115

Meeting – February 26th @ 5:30PM

1. Prof. Chris Zhu, Human Sciences, OSU
“Studies of gut bacterial metabolites using MS-based metabolomics and an in vitro human colonic model (HCM)”

2. Dr. Djawed Bennouna, Kopec Research Group, OSU
“The Study of the impact of environment and genetics on the nutritional quality of rapeseed by a metabolomic approach”

Date: February 26
Time: 5:30 PM
Place: 2015 MP (053, 140 W 18th Ave)

Meeting – January 22nd @ 5:30pm

Ms. Devin Swiner, Badu Research Group, Ohio State
“Microsampling with Cotton Threads”

Dr. Dalton Snyder, CCIC, Ohio State
“Neutral loss MS/MS scans in a linear quadrupole ion trap and application to analysis of pharmaceutical mixtures via ion/molecule reactions”

Date: January 22nd
Time: 5:30PM
Location: TBA