Fred Strathman, Sr. VP of US Business, MOBILion Systems
“The Duality of Ion Mobility: From Practical Applications to Novel Insights”
Date: Friday, April 19, 2024
Time: 12:30pm
Location: James L045
Fred Strathman, Sr. VP of US Business, MOBILion Systems
“The Duality of Ion Mobility: From Practical Applications to Novel Insights”
Date: Friday, April 19, 2024
Time: 12:30pm
Location: James L045
Dr. John Sausen, Agilent Technologies, Director of Strategic Initiatives — Mass Spectrometry
“Advancing Ion Mobility into Lipidomics, Metabolomics, Exposomics, and Protein Characterization”
Date: Friday, April 5, 2024
Time: 12:30pm
Location: 014 University Hall
Abstract
Laboratories conducting Multi-Omics analysis have challenging requirements to better define isomeric and isoberic structures across a broad mass range in a relatively short time scale – increasing throughput and reducing cost per sample. This applies to Lipidomics, Metabolomics and Proteomics as well as Protein characterization. Specifically with Native Protein Structures, it would be helpful to determine if there are multiple proteoforms present in putative proteomics biomarkers and therapeutic biomolecules. Recent advancements in Ion Mobility are making this possible. Multiplexing improves Sensitivity, dynamic range, extends the mass range to lower mass. It also enables added statistical significance enabling High Resolution Demultiplexing which can dramatically increase Drift Resolution of Isomers and Isobars while maintaining fast UHPLC acquisition rates as well as a wide mass range – single injection with both high drift resolution and excellent chromatographic fidelity. In addition, Ion Mobility can be predictive in assisting with identifications of unknowns not found in a data base. For example, leveraging high precision CCS Collisional Cross Sections of a homologous series of Lipids or PFAS subunits, or enabling Machine Learning to better predict which unknown isomer hypothesis has the most likelihood of being accurate. The better the precision in the training set, the more accurate the prediction of the putative unknown isomer – will show PFAS as an example, could apply to many isomeric compound classes. We can now achieve and leverage precision of the CCS (0.4 – 0.2%RSD – great precision in the CCS) enabling better accuracy in the predictions. Another important subject is Protein Characterization. Several techniques and assist in helping to determine if there are multiple protoeforms present – Collision Induced Unfolding, being one – compare profiles of a putative protein marker in disease Vs not diseased, or a therapeutic in a bioreactor as one optimizes changing feedstocks – quickly determine if something changed. Agilent has improved the precision to both the CCS and CIU50 Voltage values of native protein unfolding, enabling more confidence in rapidly determining if the protein structure has changed. In addition, with newer electrodes used in the CIU experiments, we are now able to work with larger native protein structures, as large as GroEL. Realtime Microdroplet flash characterization can also offer interesting tools to better rapidly characterize higher order structures.
Bio
John has been a part of Agilent Technologies and Hewlett Packard for the past 41 years. He is currently Director of Strategic Initiatives – Mass spectrometry, supporting the America’s field organization. He works with thought leaders in a a business development capacity, building collaborations and collecting customer feedback on changing requirements in the life science markets, with a focus toward triple quad, QTOF, and Ion Mobility technology and applications.
Ms. Kristie Baker, Wysocki Group, Ohio State
“Characterizing a Surface Bridge in the Four-Helix Bundle Rop by Surface- and Collision-Induced Activation”
and
Philip Lacey, Wysocki Group, Ohio State
“Heterogeneous Non-Canonical Inflammasome Oligomerization Probed by Narrow Quadrupole Selections and Electron Capture Charge Reduction”
Date: Friday, February 23, 2024
Time: 12:30pm
Location: 0035 Hitchcock Hall
Mr. Alex Grooms, Badu Group, Ohio State
“Uncatalyzed Hydrazine Cross Coupling via Plasma-Microdroplet Fusion Enables Programmable C–N Bond Formation”
Date: January 26, 2024
Time: 12:30 PM
Location 0035 Hitchcock Hall
Ms. Ayesha Seth, Badu Group, Ohio State
“Revolutionizing Malaria Detection: Pioneering Chemical Signal Amplification for Asymptomatic Cases with Mass Spectrometry”
and
Mr. Joseph Holbrook, Hummon Group, Ohio State
“Enhancement of Lipid Signals in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry with Ammonium Fluoride as a Matrix Additive”
Date: Dec 12, 2023
Time: 5:30 PM
Location: BRT 105
Ms. Ariana Shannon (Hummon/Searle Group)
“Generating fit-for-purpose targeted mass-spectrometry based proteomics assays from a global proteome catalog”
Date: Nov 14, 2023 (TODAY)
Time: 5:30 PM
Location: BRT 105
Dr. Joshua Gilbert, Wysocki Research Group, Ohio State
“Combining Soft-Landing MS and Low-Energy Electron Holography for Structural Biology”
Date: October 24, 2023
Time: 5:30 PM
Location: BRT 105
Mr. Alex Grooms, Badu Lab, Ohio State
“Programmable Microdroplet Cascade Reactions: Uncatalyzed Michael Addition for Carbon-Carbon Bond Formation via Plasma-Water Fusion”
and
Mr. Daniel Quiroz Moreno (Cooperstone Group)
“Lessons Learned from Data Dependent Acquisition and Molecular Networking: Case Application in Phenolics”
Date: September 19, 2023
Time: 5:30 PM
Location: BRT 105
Mr. Alex Grooms, Badu Lab, Ohio State
“Dual Tunability for Uncatalyzed N-alkylation of Primary Amines Enabled by Super Reactive Electrospray Droplets”
and
Mr. Brian Fries, Hummon Lab, Ohio State
“Fatty Acid Synthase Inhibition in Colorectal Cancer Spheroids”
Date: April 18, 2023
Time: 5:30 PM
Location: DHLRI 170
Dr. Marius Kostelic, Wysocki Lab, Ohio State
“Architecture of Adeno-Associated Viral Capsids with Surface-Induced Dissociation and Charge Detection Mass Spectrometry”
and
Mr. Daniel Quiroz Moreno, Cooperstone Lab, Ohio State
“MS2extract: An R Package for a Scalable MS/MS Compound Library Creation”
Date: March 28, 2023
Time: 5:30 PM EST
Location: DHLRI 170
Zihao Qi, Wysocki Lab, Ohio State, “Native Mass Spectrometry of Single Strand Annealing Protein and Nucleoprotein Complexes”
and
Enoch Amoah, Badu Lab, Ohio State, “Mass Spectrometry Approach for Differentiation of Positional Isomers of Saccharides: Toward Direct Analysis of Rare Sugars”
Date: February 21, 2022
Time: 5:30 PM Eastern
Location: DHLRI 170
We are canceling this month’s MSDG meeting. Instead, we encourage people to please attend seminars by analytical faculty candidates. Originally scheduled speakers for January will present at February’s meeting. Meeting schedule.
Emily Sekera, Hummon Lab, Ohio State. “Mass Spectrometry Imaging of 3D Cell Cultures: From Drug Treatments to Dietary Changes”
Date: December 13, 2022
Time: 5:30 PM
Location: BRT 115
Dr. Andrew Arslanian, Wysocki Group, Ohio State. “Gas Phase Techniques for the Study of Biomolecular and Supramolecular Structures and Chemistry”
Date: November 15, 2022
Time: 5:30 PM Eastern
Location: CBEC 267
Prof. David Russell, Texas A&M University, “Advanced MS Technologies for Studies for Biomolecule Complexes: Structure, Stability and Dynamics”
Date: October 3, 2022
Time: 5:30 PM EST
Location: CBEC 130
Mr. Alex Grooms, Badu Lab, Ohio State, “Plasma-Droplet Reaction Systems: A Direct Mass Spectrometry Approach for Enhanced Characterization of Lipids at Multiple Isomer Levels”
Date: September 20, 2022
Time: 5:30 PM EST
Location: CBEC 267
Prof. Anyin Li, University of New Hampshire
Date: April 19th, 2022
Time: 5:30 PM EST
Zoom: https://osu.zoom.us/j/96434729214?pwd=R0U1K3hyZ2M1a1ZYR296eVRLcTVlZz09
Meeting ID: 964 3472 9214
Password: 859213
1. Mr. Taghi Sahraeian, Badu Research Group “Air-Tight Ambient Mass Spectrometry for Direct Analysis of Solid-Electrolyte Interphase of Li-Ion Battery”
2. Second student volunteer speaker is needed
Date: March 29th, 2022
Time: 5:30 PM EST
Zoom: https://osu.zoom.us/j/96434729214?pwd=R0U1K3hyZ2M1a1ZYR296eVRLcTVlZz09
Meeting ID: 964 3472 9214
Password: 859213
Dr. Damien Wilburn, Searle Research Group, Ohio State Comprehensive Cancer Center “Deep learning of proteome chemistry through harmonization of massive peptide libraries”
Date: Feb 22nd, 2022
Time: 5:30 PM EST
Zoom: https://osu.zoom.us/j/96434729214?pwd=R0U1K3hyZ2M1a1ZYR296eVRLcTVlZz09
Meeting ID: 964 3472 9214
Password: 859213
Ms. Elaura Gustafson, Brigham Young University, Daniel Austin’s Research Group “Printed Circuit Board Charge Detection Mass Spectrometry”
Date: Jan 25th, 2022
Time: 5:30 PM EST
Zoom: https://osu.zoom.us/j/96434729214?pwd=R0U1K3hyZ2M1a1ZYR296eVRLcTVlZz09
Meeting ID: 964 3472 9214
Password: 859213
Prof. Brian Searle, Biomedical Informatics, Ohio State Comprehensive Cancer Center
Date: Dec 14th, 2021
Time: 5:30 PM EST
Zoom: https://osu.zoom.us/j/96220959486?pwd=Y2R2K2EwUTArTXZ4b1IrT21DVXJKQT09
Meeting ID: 962 2095 9486
Password: 748441
Chen Du (Wysocki Group) and Philip Lindhorst (Hummon Group)
Date: Nov 16th, 2021
Time: 5:30 PM EST
Zoom: https://osu.zoom.us/j/96220959486?pwd=Y2R2K2EwUTArTXZ4b1IrT21DVXJKQT09
Meeting ID: 962 2095 9486
Password: 748441
Prof. Ekin Atilla-Gokcumen, University at Buffalo “LC-MS-based approaches for the discovery of novel lipid regulators of cell death”
Date: Oct 12th, 2021
Time: 5:30 PM EST
Zoom: https://osu.zoom.us/j/96220959486?pwd=Y2R2K2EwUTArTXZ4b1IrT21DVXJKQT09
Meeting ID: 962 2095 9486
Password: 748441
Mr. Taghi Sahraeian, Badu Research Group “Droplet Imbibition Mass Spectrometry”
Date: Septh 14th, 2021
Time: 5:30 PM EST
Zoom: link provided via email or contact badu-tawiah.1@osu.edu
Prof. Justin Benesch, University of Oxford “Combining native mass spectrometry with mass photometry to elucidate the assembly of ultra-heterogeneous protein assemblies”
Date: March 23rd, 2021
Time: 5:30 PM EST
Zoom: link provided via email or contact badu-tawiah.1@osu.edu
Dr. Candice Ulmer, Research Chemist, CDC “The Development of Routine and Reference Measurement Procedures to Support the Accurate Reporting of Clinical Measurements”
Date: February 23rd, 2021
Time: 5:30 PM EST
Zoom: link provided via email or contact badu-tawiah.1@osu.edu
Dr. Michael Wleklinski, Senior Scientist, Merck & Co. “High Throughput Synthesis and Screening: My Journey from Academia to Industry”
Date: December 8, 2021
Time: 5:30 PM EST
Zoom: link provided via email or contact badu-tawiah.1@osu.edu
Prof. Julia Laskin, Purdue University “Ambient imaging of biological samples using nanospray desorption electrospray ionization (nano-DESI) mass spectrometry”
Date: December 8, 2020
Time: 5:30 PM EST
Zoom: link provided via email or contact badu-tawiah.1@osu.edu
1. Dr. Maya Reimi Sipala, Scientist at Balazs NanoAnalysis, “HR-ICP-MS Applications in the Semiconductor Industry”
Date: November 17, 2020
Time: 5:30 PM EST
Zoom: to be provided via email
1. Prof. Carlito Lebrilla, Department of Chemistry, UC Davis “Carbohydrates as biomarkers for health and diseases”
Date: October 13, 2020
Time: 5:30 PM (EST)
Zoom: to be provided
1. Dr. Mike Freitas
“Moving mass Spectrometry informatics into the cloud. A case study report on the implementation of AWS cloud based workflows for mass spectrometry data analysis.”
Date: Sept 15th
Time: 5:30pm
Place: Zoom – link to be provided via email
In keeping with directions provided by the University in regards to corona virus pandemic, MSDG meetings will be suspended for the remainder of the semester.
All speakers have been notified of this change
1. Prof. Jim Prell, University of Oregon
“Quantitative Protein Unfolding with a Q-TOF”
Date: February 24th
Time: 5:30 PM
Place: 105 BRT
1. Dr. Ralph Tobias, Hummon Group
“Developing a Drug Screening Platform: MALDI-Mass Spectrometry Imaging of Paper-Based Cultures”
2. Ms. Haley Chatelaine, Kopec Group
“A Targeted and Untargeted LC-MS Approach to Determining Postprandial Vitamin A&D and Lipid Levels in Metabolic Syndrome versus Healthy Subjects”
Date: January 21st
Time: 5:30 PM
Place: 105 BRT
1. Dr. Djawed Bennouna, Kopec Research Group, OSU
“The Effects of Omega-3 Supplementation on the Brain Lipids after Chemotherapy”
2. Dr. Dmytro Kulyk, Badu Research Group, OSU
“High-Throughput Mass Spectrometry Platform for Gas-Phase Reaction Screening under Ambient Conditions”
Date: November 26
Time: 5:30 PM
Place: 105 BRT
Topic: Thermal ionization mass spectrometry methods and applications in the earth and ocean sciences
Overview by Prof. Elizabeth Griffith, School of Earth Sciences, OSU
Application by Ms. Samantha Carter, Griffith Research Group, OSU
Date: September 24
Time: 5:30 PM
Place: JAMES B050 (460 W. 10TH Ave)
Mr. Taghi Sahraeian, Badu Research Group, OSU
“Droplet Imbibition Enables Non-Equilibrium Interfacial Reactions in Charged Microdroplets”
Date: August 27th
Time: 5:30 PM
Place: 105 BRT
COH-MSDG – Central Ohio (OH) Mass Spectrometry Discussion Group
1. Mr. Ben Burris, Badu Research Group, OSU
“Probing Kinetics of Biomolecular Reactions via Contained-ESI Mass Spectrometry”
2. Ms. Maddy Lomax-Vogt, Olesik Research Group, OSU
“Inductively Coupled Plasma Fundamentals and Nanoparticle Measurements”
Date: April 23
Time: 5:30 PM
Place: 2015 MP
Prof. Jenny Brodbelt, Department of Chemistry, The University of Texas at Austin
“Lipid analysis using UVPD”
Date: March 25
Time: 5:30 PM
Place: BRT 115
1. Prof. Chris Zhu, Human Sciences, OSU
“Studies of gut bacterial metabolites using MS-based metabolomics and an in vitro human colonic model (HCM)”
2. Dr. Djawed Bennouna, Kopec Research Group, OSU
“The Study of the impact of environment and genetics on the nutritional quality of rapeseed by a metabolomic approach”
Date: February 26
Time: 5:30 PM
Place: 2015 MP (053, 140 W 18th Ave)
Ms. Devin Swiner, Badu Research Group, Ohio State
“Microsampling with Cotton Threads”
Dr. Dalton Snyder, CCIC, Ohio State
“Neutral loss MS/MS scans in a linear quadrupole ion trap and application to analysis of pharmaceutical mixtures via ion/molecule reactions”
Date: January 22nd
Time: 5:30PM
Location: TBA
Dr. Arpad Somogyi, CCIC, Ohio State
“Availability of a new ion mobility technique TIMS (trapped ion mobility spectrometry), in the CCIC Mass Spec and Proteomics Facility: implementation on a Q-TOF and an FT-ICR instrument ”
Prof. Abraham Badu-Tawiah, Badu Research Group, Ohio State
“New Approaches for Ultra-small Volume Analysis by Mass Spectrometry”
Date: December 4th
Time: 5:30PM
Location: TBA
Prof. Heather Desaire, University of Kansas, Lawrence
“Combining Machine Learning, Mass Spectrometry, and Glycomics to Diagnose Disease”
Date: November 11th
Time: 5:30PM
Location: TBA
Prof. Christian Bleiholder, Florida State University, Tallahassee
“Protein structure analysis with ion mobility spectrometry/mass spectrometry: what are the challenges?”
Date: October 1st
Date: 5:30PM
Location: TBA
Ms. Samantha Sarni, Wysocki Research Group, Ohio State
“Unlocking the Mechanism of HIV-1 Viral Assembly Initiation with Native Mass Spectrometry”
Date: September 5th
Time: 5:30PM
Location: TBA
Prof. Lars Konnerman, The University of Western Ontario, London
“From Solution into the Gas Phase: A Look at Electrosprayed Protein Ions and ESI Mechanisms”
Date: April 23rd
Time: 5:30PM
Location: TBA
Prof. Laura Reed, University of Alabama, Tuscaloosa
“Disentangling the gene-by-environment interaction architecture of metabolic disease through the lens of evolutionary genetics and metabolomics”
Date: March 29th
Time: 5:30PM
Location: TBA
Mr. William Andrews, Hummon Research Group, Ohio State
“Enhancing Mass Spectrometric Molecular Imaging Through Active Analyte Extraction”
Ms. Sierra Jackson, Badu Research Group, Ohio State
“Thread Spray Mass Spectrometry for Direct Analysis of Capsaicinoids in Pepper Products”
Date: February 20th
Time: 5:30PM
Location: TBA
Mrs. Bernice Agana, Wysocki Research Group, Ohio State
“Multi-omics Profiling of LKB1 Mutant NSCLC Cells to Characterize Deregulated Pathways Contributing to Tumorigenesis”
Mr. Dmytro Kulyk, Badu Research Group, Ohio State
“Multi-mode Nano-Contained Ion Source Capable of ESI and APCI”
Date: January 23rd
Time: 5:30PM
Location: TBA