CCIC Mass Spectrometry and Proteomics Summer Workshop

A Mass Spectrometry and Proteomics Summer Workshop will be held on July 15-16 in DHLRI 170, located at 473 W. 12th Ave, on the OSU campus in Columbus, OH. The two-day workshop is presented by faculty and staff of the OSU Campus Chemical Instrument Center Mass Spectrometry and Proteomics Facility.

All students, postdocs, faculty, and research staff interested in an introduction to mass spectrometry and proteomics are invited to participate. There is no cost to attend, but registration is required.

Please visit https://u.osu.edu/mspworkshop for agenda, link to registration, and additional information.

April 19 @12:30pm

Fred Strathman, Sr. VP of US Business, MOBILion Systems

“The Duality of Ion Mobility: From Practical Applications to Novel Insights”

Date: Friday, April 19, 2024
Time: 12:30pm
Location: James L045

April 5 @12:30pm

Dr. John Sausen, Agilent Technologies, Director of Strategic Initiatives — Mass Spectrometry

“Advancing Ion Mobility into Lipidomics, Metabolomics, Exposomics, and Protein Characterization”

Date: Friday, April 5, 2024
Time: 12:30pm
Location: 014 University Hall

Abstract
Laboratories conducting Multi-Omics analysis have challenging requirements to better define isomeric and isoberic structures across a broad mass range in a relatively short time scale – increasing throughput and reducing cost per sample. This applies to Lipidomics, Metabolomics and Proteomics as well as Protein characterization. Specifically with Native Protein Structures, it would be helpful to determine if there are multiple proteoforms present in putative proteomics biomarkers and therapeutic biomolecules. Recent advancements in Ion Mobility are making this possible. Multiplexing improves Sensitivity, dynamic range, extends the mass range to lower mass. It also enables added statistical significance enabling High Resolution Demultiplexing which can dramatically increase Drift Resolution of Isomers and Isobars while maintaining fast UHPLC acquisition rates as well as a wide mass range – single injection with both high drift resolution and excellent chromatographic fidelity. In addition, Ion Mobility can be predictive in assisting with identifications of unknowns not found in a data base. For example, leveraging high precision CCS Collisional Cross Sections of a homologous series of Lipids or PFAS subunits, or enabling Machine Learning to better predict which unknown isomer hypothesis has the most likelihood of being accurate. The better the precision in the training set, the more accurate the prediction of the putative unknown isomer – will show PFAS as an example, could apply to many isomeric compound classes. We can now achieve and leverage precision of the CCS (0.4 – 0.2%RSD – great precision in the CCS) enabling better accuracy in the predictions. Another important subject is Protein Characterization. Several techniques and assist in helping to determine if there are multiple protoeforms present – Collision Induced Unfolding, being one – compare profiles of a putative protein marker in disease Vs not diseased, or a therapeutic in a bioreactor as one optimizes changing feedstocks – quickly determine if something changed. Agilent has improved the precision to both the CCS and CIU50 Voltage values of native protein unfolding, enabling more confidence in rapidly determining if the protein structure has changed. In addition, with newer electrodes used in the CIU experiments, we are now able to work with larger native protein structures, as large as GroEL. Realtime Microdroplet flash characterization can also offer interesting tools to better rapidly characterize higher order structures.

Bio
John has been a part of Agilent Technologies and Hewlett Packard for the past 41 years. He is currently Director of Strategic Initiatives – Mass spectrometry, supporting the America’s field organization. He works with thought leaders in a a business development capacity, building collaborations and collecting customer feedback on changing requirements in the life science markets, with a focus toward triple quad, QTOF, and Ion  Mobility technology and applications.

 

February 23 @12:30pm

Ms. Kristie Baker, Wysocki Group, Ohio State
“Characterizing a Surface Bridge in the Four-Helix Bundle Rop by Surface- and Collision-Induced Activation”

and

Philip Lacey, Wysocki Group, Ohio State
“Heterogeneous Non-Canonical Inflammasome Oligomerization Probed by Narrow Quadrupole Selections and Electron Capture Charge Reduction”

Date: Friday, February 23, 2024
Time: 12:30pm
Location: 0035 Hitchcock Hall

January 26 @12:30pm

Mr. Alex Grooms, Badu Group, Ohio State
“Uncatalyzed Hydrazine Cross Coupling via Plasma-Microdroplet Fusion Enables Programmable C–N Bond Formation”

Date: January 26, 2024
Time: 12:30 PM
Location 0035 Hitchcock Hall

December 12 @5:30pm

Ms. Ayesha Seth, Badu Group, Ohio State
“Revolutionizing Malaria Detection: Pioneering Chemical Signal Amplification for Asymptomatic Cases with Mass Spectrometry”

and

Mr. Joseph Holbrook, Hummon Group, Ohio State
“Enhancement of Lipid Signals in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry with Ammonium Fluoride as a Matrix Additive”

Date: Dec 12, 2023
Time: 5:30 PM
Location: BRT 105

Meeting — November 14 @5:30pm

Ms. Ariana Shannon (Hummon/Searle Group)

“Generating fit-for-purpose targeted mass-spectrometry based proteomics assays from a global proteome catalog”

Date: Nov 14, 2023 (TODAY)
Time: 5:30 PM
Location: BRT 105

Meeting — October 24 @5:30pm

Dr. Joshua Gilbert, Wysocki Research Group, Ohio State

“Combining Soft-Landing MS and Low-Energy Electron Holography for Structural Biology”

Date: October 24, 2023
Time: 5:30 PM
Location: BRT 105

Meeting — September 19 @5:30pm

Mr. Alex Grooms, Badu Lab, Ohio State
“Programmable Microdroplet Cascade Reactions: Uncatalyzed Michael Addition for Carbon-Carbon Bond Formation via Plasma-Water Fusion”

and

Mr. Daniel Quiroz Moreno (Cooperstone Group)
“Lessons Learned from Data Dependent Acquisition and Molecular Networking: Case Application in Phenolics”

Date: September 19, 2023
Time: 5:30 PM
Location: BRT 105

Meeting — April 18 @5:30pm

Mr. Alex Grooms, Badu Lab, Ohio State
“Dual Tunability for Uncatalyzed N-alkylation of Primary Amines Enabled by Super Reactive Electrospray Droplets”

and

Mr. Brian Fries, Hummon Lab, Ohio State
“Fatty Acid Synthase Inhibition in Colorectal Cancer Spheroids”

Date: April 18, 2023
Time: 5:30 PM
Location: DHLRI 170

Meeting — March 28 @5:30pm

Dr. Marius Kostelic, Wysocki Lab, Ohio State
“Architecture of Adeno-Associated Viral Capsids with Surface-Induced Dissociation and Charge Detection Mass Spectrometry”

and

Mr. Daniel Quiroz Moreno, Cooperstone Lab, Ohio State
“MS2extract: An R Package for a Scalable MS/MS Compound Library Creation”

Date: March 28, 2023
Time: 5:30 PM EST
Location: DHLRI 170

Meeting — February 21 @5:30pm

Zihao Qi, Wysocki Lab, Ohio State, “Native Mass Spectrometry of Single Strand Annealing Protein and Nucleoprotein Complexes”

and

Enoch Amoah, Badu Lab, Ohio State, “Mass Spectrometry Approach for Differentiation of Positional Isomers of Saccharides: Toward Direct Analysis of Rare Sugars”

Date: February 21, 2022
Time: 5:30 PM Eastern
Location: DHLRI 170

Meeting — December 13 @5:30pm

Emily Sekera, Hummon Lab, Ohio State. “Mass Spectrometry Imaging of 3D Cell Cultures: From Drug Treatments to Dietary Changes”

Date: December 13, 2022
Time: 5:30 PM
Location: BRT 115

Meeting — November 15 @5:30pm

Dr. Andrew Arslanian, Wysocki Group, Ohio State. “Gas Phase Techniques for the Study of Biomolecular and Supramolecular Structures and Chemistry”

Date: November 15, 2022
Time: 5:30 PM Eastern
Location: CBEC 267

Meeting — October 3rd @5:30pm

Prof. David Russell, Texas A&M University, Advanced MS Technologies for Studies for Biomolecule Complexes: Structure, Stability and Dynamics”

Date: October 3, 2022
Time: 5:30 PM EST
Location: CBEC 130

Meeting – Sept 20th @ 5:30pm

Mr. Alex Grooms, Badu Lab, Ohio State, “Plasma-Droplet Reaction Systems: A Direct Mass Spectrometry Approach for Enhanced Characterization of Lipids at Multiple Isomer Levels”

Date: September 20, 2022
Time: 5:30 PM EST
Location: CBEC 267