Publications detailing nuclear 18S rRNA sequences of Acanthamoeba in the DNA databases

PRIMARY REFERENCES FOR MOLECULES THAT ARE USED IN PHYLOGENETIC ANALYSIS OF ACANTHAMOEBA:

Nuclear Small Subunit rRNA (SSU; 18S rRNA; Rns):

Gunderson, J.H. and Sogin, M.L. 1986.    Length variation in eukaryotic rRNAs: Small subunit rRNAs from the protists Acanthamoeba castellanii and Euglena gracilisGene 44: 63-70.  (accession # M13435)
 

ADDITIONAL REFERENCES FOR 18S rRNA GENE SEQUENCES IN THE DNA DATABASES:

(over 600 references; partially updated December 2023 – recent [2023] attempts to update continuing)

Abass, Z.F. and Jasim, G.A., 2023. Apportunistic identification of a free-living Acanthamoeba spp. clinical in different animales and human in Karbala and Qadisiyah province/Iraq.  Journal of Survey in Fisheries Sciences, 10(3S), pp.4902-4915  (acc  OQ679416 – OQ679418).

Abd El Wahab,W.M., El-Badry, A.A. and Hamdy, D.A. 2018. Molecular characterization and phylogenetic analysis of Acanthamoeba isolates in tap water of Beni-Suef, Egypt. Acta Parasitologica, 2018, 63(4), 826–834.  (accession # MH331619 – MH331638).

Abe,N. and Kimata,I. 2008. Genotype identification of the Acanthamoeba isolates from patients with keratitis in Osaka, Japan. Unpublished. (accession #’s AB447386 – AB447388).

Abe, N. and I. Kimata. 2010. Genotyping of Acanthamoeba Isolates from Corneal Scrapings and Contact Lens Cases of Acanthamoeba Keratitis Patients in Osaka, Japan.  Jpn. J. Infect. Dis., 63 (4), 299-301. (accession #’s  AB554223 – AB554229).

Abdul Majid,M.A., Mahboob,T., Mong,B.G., Jaturas,N., Richard,R.L., Tian-Chye,T., Phimphila,A., Mahaphonh,P., Aye,K.N., Aung,W.L., Chuah,J., Ziegler,A.D., Yasiri,A., Sawangjaroen,N., Lim,Y.A. and Nissapatorn,V. 2017. Pathogenic waterborne free-living amoebae: An update from selected Southeast Asian countries. PLoS ONE 12:(2) E0169448.  (accession # KX232517 – KX232520).

Abedkhojasteh, H., Niyyati, M., Rezaian, M., Heidari,M., Mirjalali,H., Rezaie,S. and Mohebali,M. 2012. unpublished. (accession # JQ903617).

Abokhalil,N.A.  2015.  Direct Submission, Parasitology, Menoufiya University, Gamal Abdel Nasser, Shebein El Koum, Menoufiya 23511, Egypt  (accession # KR270798).

Adamska, M.   2016.  Molecular Characterization of Acanthamoeba spp. Occurring in Water Bodies and Patients in Poland and Redefinition of Polish T16 Genotype.  Journal of Eukaryotic Microbiology 63: 262–270.  (accession # KR780546 – KR780556.  KR780557 – KR780558, KR780559 – KR780569).

Adamska, M., Leonska-Duniec, A., Lanocha, N., Skotarczak, B.  2014.  Thermophilic potentially pathogenic amoebae isolated from natural water bodies in Poland and their molecular characterization.  Acta Parasitol. 59: 433-441. (Accession #’s HQ632775 – HQ632781;  JQ408984 – JQ409014).

Aghajani, A., Hooshyar, H., Dabirzadeh, M. and Maroofi, Y.  2015.  Acanthamoeba 18S ribosomal RNA genes from Isfahan Iran.  Unpublished. (accession #LC084752, LC085292, LC085425, LC085426)

Akya, A., Pointon, A. and Thomas, C. 2009 Viability of Listeria monocytogenes in co-culture with Acanthamoeba spp. FEMS Microbiology Ecology 70: 20-29.  (Accession #’s DQ490964EF175998 – EF176006).

Al-Herrawy, A.Z. and Gad, M.A. 2015.  Genotyping of Acanthamoeba spp. isolated from swimming pools, Egypt. Unpublished. (accession # KR062066).

Al-Herrawy AZ, Khalil MI, El-Sherif SS, Omar FAE, Lotfy WM. 2017. Surveillance and Molecular Identification of Acanthamoeba and Naegleria Species in Two Swimming Pools in Alexandria University, Egypt.  Iranian Journal of Parasitology 12(2):196-205. (accession # KU312268 – KU312277).

Al-Herrawy, A.Z., Lotfy, W.M., Abou EL-Hoda, M., Abu Kabsha, S. and Gad, M.A. 2019. Molecular detection of Acanthamoeba species isolated from water in Damanhour city. Unpublished; Water pollution research, National Research Centre, El Bohouth, Giza, Egypt. (acc #’s MK880493 – MK880495; MK894130).

Alves, J.M.P., Teixeira, M.M.G., Affonso, H.T., Nunes,L.R. and  Oliveira, R.L.C.  2001.  Acanthamoeba phylogeny and species determination by SSU rDNA sequencing: two new sequence types.  Unpublished.  FROM:  Alves, J.M.P. 2001. Caracterização e filogenia moleculares de Acanthamoeba. Doctoral Thesis, Instituto de Ciências Biomédicas, Universidade de São Paulo, Brazil  (accession #’s AY026242 – AY026749).

Alves D de,S., Moraes,A.S., Nitz,N., de Oliveira,M.G., Hecht,M.M., Gurgel-Goncalves,R. and Cuba,C.A.. 2012. Occurrence and characterization of Acanthamoeba similar to genotypes T4, T5, and T2/T6 isolated from environmental sources in Brasilia, Federal District, Brazil.  Experimental Parasitology 131: 239-244.  (accession # JF960525; JQ268232 – JQ268248).

Alves,D.S. 2012. cornea of a patient with keratitis. unpublished. (accession # JQ791107).

Amaral Zettler, L.A., Anderson, O.R., Nerad, T.A. and Sogin, M.L.  2001.  The phylogenetic position of Comandonia operculata and its implications for the taxonomy of the genus Acanthamoeba.  (in): IXth International Meeting on the Biology and Pathogenicity of Free-living Amoebae Proceedings: 235-242;  John Libbey Eurotext, Paris, France.  (accession # AY033896).

Amaro, F., Wang, W., Gilbert, J.A., Anderson, O.R. and Shuman, H.A. 2014. Diverse protist grazers select for virulence-related traits in Legionella. Unpublished. (accession # KF607041)

Anand, D.A. and Sundararaj, T.    2006.  Acanthamoeba from Tamilnadu, India. (Unpublished).  (Accession #’s EF050468 – EF050508).

 

Armand, B., Motazedian, M.H. and Asgari, Q. 2016. Isolation and identification of pathogenic free- living amoeba from surface and tap water of Shiraz city using morphological and molecular methods. Parasitology Research 115: 63-68. (accession # KM823777 – KM823785).

Arnalich-Montiel, F., Lumbreras-Fernández, B., Martín-Navarro, C.M., Valladares, B., Lopez-Velez, R., Morcillo-Laiz, R., Lorenzo-Morales, J. 2014. Influence of Acanthamoeba Genotype on Clinical Course and  Outcomes of Patients with Acanthamoeba Keratitis in Spain.  J. Clin. Microbiol.  52: 1213-1216. (sequences never deposited).

Asif, M. 2017. Direct Submission. Biomedical Science, University of Edinburgh, Edinburgh, United Kingdom. (accession #MF065862 – MF065941;  MF470242 – MF470325MG022779 – MG022862).

Astorga, B., Lorenzo-Morales, J., Martin-Navarro, C.M., Alarcon, V., Moreno, J., Gonzalez, A.C., Navarrete, E., Pinero, J.E. and Valladares, B. 2011. Acanthamoeba belonging to T3, T4, and T11: genotypes isolated from air-conditioning units in Santiago, Chile. Journal of Eukaryotic Microbiology 58:  542-544. (accession # HQ236501 – HQ236530).   

Astorga, B., Goni, M.P., Lagos, J., Fuenzalida, E., Clavel, A. and Fernandez, J.  2016.  Genotyping of Acanthamoeba Strains from Environmental Sources in Chile. Unpublished.  (accession # KT892757 – KT892772KT892773 – KT892926 ).

Azzam, R., Badenoch, P.R., Francis, M.J., Fernandez, C., Adamson, P.J., Dendle, C., Woolley, I., Robson, J., Korman, T.M. and Graham, M. 2015. Acanthamoeba encephalitis: isolation of genotype T1 in mycobacterial liquid culture medium. J. Clin. Microbiol. 53: 735-739.  (accession # KM015457).

Bahreini, M., Bamdad, S., Asgari, Q., Abbaszadeh Afshar, M. and Motazedian, M. 2019. Genotyping of Acanthamoeba sp. isolated from keratitis patient by PCR method Unpublished; Medical Parasitology, Shiraz Medical University, Shiraz, Fars, Iran. (accession # MK356382)

Balczun, C. and Scheid, P.L. 2016-2018. Genotyping of Acanthamoeba isolates from clinical samples in Germany. Unpublished. Laboratory of Medical Parasitology, Central Institute of the Bundeswehr Medical Service, Koblenz, Germany.  (accession # KU935611 – KU935612;  KY498610 – KY498611; KY563326; KY569460 ; MH041313; MH044087).

Balczun, C., Riester, T., Sinsch, U. and Scheid, P. 2018. Acanthamoeba sp. genotypes from Mali. Unpublished; Laboratory of Medical Parasitology, Central Military Hospital Koblenz, Germany.  (accession # MH889156 – MH889161).

Balczun, C. and Scheid, P.L. 2018. Genotyping of Acanthamoeba isolates from clinical samples in Germany. Unpublished; Laboratory of Medical Parasitology, Central Military Hospital Koblenz, Koblenz, Germany.  (accession # MH923345).

Balczun, C., Sinsch, U., Klunke, H., Ferse, M. and Scheid, P.L. 2018. Acanthamoeba sp. genotypes from Zanzibar, Tanzania. Unpublished; Laboratory of Medical Parasitology, Central Military Hospital Koblenz, Koblenz, Germany.  (acc # MH044085 – MH044086)

Balczun, C., Sinsch, U., Klunke, H. and Scheid, P.L. 2018. Acanthamoeba sp. genotypes from Rwanda. Unpublished; Laboratory of Medical Parasitology, Central Military Hospital Koblenz, Koblenz, Germany. (acc # MH041259; MH041260; MH044076 – MH044084).

Baldwin, D. S., Colloff, M.J., Rees, G.N., Chariton, A.A.,  Watson,G.O., Court, L.N., Hartley, D.M., Morgan, M.J., King, A.J., Wilson, J.S., Hodda, M., and Hardy, C.M. 2013. Impacts of inundation and drought on eukaryote biodiversity in semi-arid floodplain soils. Molecular Ecology 22: 1746–1758. (accession # HQ191705 – HQ195362). 

Baquero,R.A., Reyes-Batlle,M., Nicola,G.G., Martin-Navarro,C.M., Lopez-Arencibia,A., Guillermo Esteban,J., Valladares,B., Martinez-Carretero,E., Pinero,J.E. and Lorenzo-Morales,J. 2014. Presence of potentially pathogenic free-living amoebae strains from well water samples in Guinea-Bissau. Pathogens and Global Health 108: 206-211. (accession # KJ000391 – KJ000394).

Barete,S., Combes,A., de Jonckheere,J.F., Datry,A., Varnous,S., Martinez,V., Garcia Ptacek,S., Caumes,E., Capron,F., Frances,C., Gibert,C. and Chosidow,O. (2007) Fatal Disseminated Acanthamoeba lenticulata Infection in a Heart Transplant Patient. Emerging Infectious Diseases 13:736-738.  (accession # AM411530).  

Basher, M.H.A., Ithoi, I., Mahmud, R., Abdulsalam, A.M., Foead, A.I., Dawaki, S., Atroosh, W.M.M., Nissapatorn, V. and Abdullah, W.O. 2017. Occurrence of Acanthamoeba genotypes in Central West Malaysian environments. Acta Trop. 178, 219-228. (accession # MF100899 – MF100909; representing 192 isolates).

Begg, A.P., Todhunter, K., Donahoe, S.L., Krockenberger, M. and Slapeta, J.  2014. Severe Amoebic Placentitis in a Horse Caused by an Acanthamoeba hatchetti Isolate Identified Using Next-Generation Sequencing. J. Clin. Microbiol. 52 (8), 3101-3104.  (accession #KJ801938).

Beier, C.L., Horn, M., Michel, R., Schweikert, M., Gortz, H.D. and Wagner, M. 2002.  The Genus Caedibacter Comprises Endosymbionts of Paramecium spp. Related to the Rickettsiales (Alphaproteobacteria) and to Francisella tularensis (Gammaproteobacteria). Applied Environmental Microbiology 68: 6043-6050.  (accession # AY102615). 

Behera HS, Panda A, Satpathy G, Bandivadekar P, Vanathi M, Agarwal T, Nayak N, Tandon R. 2016.  Genotyping of Acanthamoeba spp. and characterization of the prevalent T4 type along with T10 and unassigned genotypes from amoebic keratitis (AK) patients in India.   Journal of Medical Microbiology 65: 370-376.  (accession #’s KR809852 – KR809871).

Behera, H.S., Satpathy, G., Tripathi, M.    2015.  Isolation and genotyping of Acanthamoeba spp. from Acanthamoeba meningitis /  meningoencephalitis (AME) patients in India.  Parasites & Vectors (2016) 9:442 . (accession #’s KT004416 – KT004421)

Behniafar,H., Niyyati,M. and Lasjerdi,Z. 2015. Molecular Characterization of Pathogenic Acanthamoeba Isolated from Drinking and Recreational water in East Azerbaijan, Northwest Iran. Environ Health Insights 9, 7-12. (accession # KR074213 – KR074230).

Benito,M., Ormad,M.P., Fernandez,M.T., Rubio,E., Miguel,N. and Goni,P. 2018. Free living amoeba in Dreissena polymorpha. Unpublished; Microbiology, Preventive Medicine and Public Health, University of Zaragoza, Zaragoza, Spain.  (accession # MH678804 – MH678806).

Berry, D., Horn, M., Xi, C. and Raskin, L. 2010.  Mycobacterium avium Infections of Acanthamoeba Strains: Host Strain Variability, Grazing-Acquired Infections, and Altered Dynamics of Inactivation with Monochloramine. Applied and Environmental Microbiology 76: 6685-6688.  (accession #’s FJ807647 – FJ807651) 

Boonman, N., Prachya, S., Boonmee, A., Kittakoop, P., Wiyakrutta, S., Sriubolmas, N., Warit, S., Chusattayanond, A.D.A. 2012. In Vitro Acanthamoebicidal Activity of Fusaric Acid and Dehydrofusaric Acid from an Endophytic Fungus Fusarium sp Tlau3.  Planta Medica 78: 1562-1567. (accession #’s JX043488 – JX043490; JX104341). 

Booton,G.C., Kelly,D.J., Chu,Y.W., Seal,D.V., Houang,E., Lam,D.S., Byers,T.J. and Fuerst,P.A. 2002.  18S ribosomal DNA typing and tracking of Acanthamoeba species isolates from corneal scrape specimens, contact lenses, lens cases, and home water supplies of Acanthamoeba keratitis patients in Hong Kong.  Journal of Clinical Microbiology 40: 1621-1625.  (accession # AF441793 – AF441814)    

Booton, G.C.,  A. Rogerson, T.D. Bonilla, D.V. Seal, D.J. Kelly, T.K. Beattie, A. Tomlinson, F. Lares-Villa, P.A. Fuerst, T.J. Byers.  2004.   Molecular and Physiological Evaluation of Subtropical Environmental Isolates of Acanthamoeba spp., Causal Agent of Acanthamoeba Keratitis.  Journal of Eukaryotic Microbiology 51: 192-200. (accession #’s AY343661 – AY343684).  

Booton, G.C., Joslin, C.E., Shoff, M.E., Tu, E.Y., Kelly, D.J. and Fuerst, P.A.  2009.  Genotypic Identification of Acanthamoeba sp. Isolates Associated with an Outbreak of Acanthamoeba keratitis.  Cornea  28: 673-676. (accession #’s EU168067 – EU168082). 

Booton, G.C., Visvesvara, G.S., Byers, T.J., Kelly, D.J. and Fuerst, P.A.  2005. Identification and distribution of Acanthamoeba species genotypes associated with nonkeratitis infections. J. Clin. Microbiol. 43; 1689-1693. (accession #’s  AY702982 – AY703030). 

Borella da Silva, T.C., Dos Santos, D.L. and Rott, M.B., 2023. First report of free-living amoebae in sewage treatment plants in Porto Alegre, southern Brazil. Journal of Water and Health, 21(10), pp.1611-1624.  (accession OR233173 – OR241303; OR243669; OR288523; OR288579; OR288588; OR288650; OR289670; OR364831)

Borgonie, G., Linage, B., Ojo, A., Shivambu, S., Kuloyo, K., Cason, E., Maphanga, S., Vermeulen, J., Litthauer, D., Ralston, C., Onstott, T.C., Sherwood-Lollar, B. and Van Heerden, E.  2014. Monhystrella parvella (Eumetazoa: Nematoda) survives inside a CaCO3 stalactite at -1.4 km in Beatrix Gold Mine (South Africa) and is of marine origin. Unpublished.  (Accession # KJ546077).

Buchele, M.L.C., Wopereis, D.B., Casara, F., de Macedo, J.P., Rott, M.B., Monteiro, F.B.F., Bazzo,.M.L., Dos Reis Spada, F., Dos Santos, J., and Caumo,K.S. 2018. Contact lens-related polymicrobial keratitis: Candida albicans and Acanthamoeba spp. genotype T4. Parasitol Res.  117(11): 3431-3436.  (accession #  MF579529).

Buerano, C.C., Trinidad, A.D., Fajardo, L.S.N., Cua, I.Y., Baclig, M.O. and Natividad, F.F.  2014. Isolation of Acanthamoeba genotype T4 from a non-contact lens wearer from the Philippines.   Trop Med Health 42: 145-147.  (accession # KJ995957).

Buerano, C.C., Trinidad, A.D., Baclig, M.O. and Natividad, F.F. 2014. Molecular identification and genotyping of Acanthamoeba species isolated from nasal swabs in the Philippines. Unpublished.  (Accession #’s KM386884 – KM386889).

Buerano, C.C., Trinidad, A.D., Baclig, M.O. and Natividad, F.F. 2015. Molecular characterization of Acanthamoeba isolated from keratitis patients, hot spring and nasal swabs from human population in the Philippines. Unpublished. (Accession #KP688374).

Burgess, D. and Molestina, R. 2015. ATCC 18S rRNA gene sequences. Personal communication.  Sequences of ATCC 50435, ATCC 50662, ATCC 50664, ATCC 50665, ATCC 50669, ATCC 50670, ATCC 50675, ATCC PRA-2, ATCC PRA-12. (not deposited in DNA databases). 

Cabello-Vilchez, A.M., Martin-Navarro, C.M., Lopez-Arencibia, A., Reyes-Batlle, M., Gonzalez, A.C., Guerra, H., Gotuzzo, E., Valladares, B., Pinero, J.E. and Lorenzo-Morales, J. 2014.  Genotyping of potentially pathogenic Acanthamoeba strains isolated from nasal swabs of healthy individuals in Peru.  Acta Trop. 130: 7-10.  (accession # KF294885 – KF294903).

Cabello-Vílchez, A.M. and Lic, M.I.R.R., 2023. Molecular analysis unmasking a Balamuthia mandrillaris: Skin lesion and granulomatous amebic encephalitis by Acanthamoeba sp close to genotype T4 with fatal outcome. Clinical Infection in Practice, p.100246. (accession OP809632).

Calusinska, M., Goux, X., Fossepree, M., Muller, E.E.L., Wilmes, P., and Delfosse, P. 2018. A year of monitoring 20 mesophilic full-scale bioreactors reveals the existence of stable but different core microbiomes in bio-waste and wastewater anaerobic digestion systems. Biotechnology for Biofuels 11:196. (accession #’s KU656706, KU656817, KU656870, KU657200, KU657300, KU657334, KU657404, KU657438, KU657618,KU657811, KU657882, KU657980, KU657982, KU657989, KU658040, KU658126, KU658150, KU658271, KU658286, KU658350, KU658380, KU658404, KU658499, KU658707, KU658839, KU658912, KU658979).

Cardoso, I.R., De Lima, C.S., Dos Reis, R.B., Pinto, A.C.A., Pissinatti, T., Kugelmeier, T., da Costa Neto, S.F., Da Silva, F.A. and Santos, H.L.C., 2023. Occurrence of Free-Living Amoebae in Non-Human Primate Gut. PRE-PRINT.  (accession # OR685532 – OR685536).

Carlesso, A.M., Artuso, G., Caumo, K. and Rott, M.B. 2010. Potentially pathogenic Acanthamoeba isolated from a hospital in Brazil. Current Microbiology 60: 185-190. (Accession # GQ999992 – GQ999997).    

Carlesso, A.M. Sr., Mentz, M.B., Machado, M.L., Carvalho, A., Nunes, T.E.T., Maschio, V.J. and Rott, M.B. 2014. Characterization of Isolates of Acanthamoeba from the Nasal Mucosa and Cutaneous Lesions of Dogs. Curr Microbiol (2014) 68:702–707. (accession # KF054072 – KF054078; KF962047 – KF962052). 

Caumo, K.S. and Rott, M.B. 2011. Acanthamoeba T3, T4 and T5 in swimming-pool waters from Southern Brazil. Acta Tropica 117: 233-235. (accession # HM055885 – HM055897).   

Celis, C.M., Caburnay, I.M., Avila, F.I., Conol, H.M., Cuevas, A., Cunan, M.A., Blanco, J.P., Banal, K.B., Hapan, M.F.Z., Manahan, E.P. and Padua, M.F., 2023. First report of Acanthamoebae spp. isolation from a volcanic mud spring in the Philippines. Journal of Water and Health, 21(11), pp.1735-1740.  (acc # OR447488).

Chan,L.L., Mak,J.W., Mohamed Kamel, A.G., Ithoi,I. and Mohamed,S.M. 2009. Molecular characterization of two Acanthamoeba isolates obtained from keratitis lesion in Malaysia.   Unpublished. (accession # GQ304896 – GQ304897).

Chan LL, Mak JW, Low YT, Koh TT, Ithoi I, Mohamed SM. 2011. Isolation and characterization of Acanthamoeba spp. from air-conditioners in Kuala Lumpur, Malaysia.  Acta Tropica 117 :23-30. (accession #’s FJ970627 – FJ970647).  

Chan, L.L., Toh, H.C., Jaikwang, K., Loo, E.M., Wong, J.H., Liew, Y.K., Ong, K.H. and Ooi, S.S., 2022. Report of rare genotypes of Acanthamoeba from soil source of the Payeh Maga Highland forest, North-eastern Sarawak, Malaysia. Acta Tropica, 229, p.106372. (acc MZ686712 – MZ686722)

Chariton,A.A., Court,L.N., Hartley,D.M., Colloff,M.J. and Hardy,C.M. 2011. Ecological assessment of estuarine sediments by pyrosequencing eukaryotic ribosomal DNA.  Front.  Ecol. Environ.  8 (5), 233-238(accession # FJ986957). 

Charnock, C., Otterholt, E. and Nordlie, A.-L. 2012. Tap Inner Surfaces as Vectors of Microbial Transmission. Unpublished. (accession # JQ361080 – JQ361083).

Chusattayanond, A.D., Boonsilp, S., Kasisit, J., Boonmee, A. and Warit, S. 2010. Thai Acanthamoeba isolate (T4) induced apoptotic death in neuroblastoma cells via the Bax-mediated pathway. Parasitology International 59: 512-516. (accession # HM043808). 

Chusattayanond, A.D., Boonmee,A., Warit,S. and Buates,S. 2012. Genotyping of Acanthamoeba isolates from soil and corneal scrapings in Thailand. Unpublished.  (accession # HM048977 – HM048983).

Choi, M.S., Myong, N.H., Seo, M., Jang, S., Yun, D.K., Yeom, K., Chung, D.I., Park, B.C., Hong, Y. and Kim, M.H., 2023. The First Case of Cutaneous Acanthamoebiasis Caused by Acanthamoeba triangularis in Korea. Annals of Dermatology, 35(Suppl 2), p.S275. (acc # MZ310461)

Choi, S.H., Cho, M.K., Ahn, S.C., Lee, J.E., Lee, J.S., Kim, D.H., Xuan, Y.H., Hong, Y.C., Kong, H.H., Chung, D.I., Yu, H.S. 2009. Endosymbionts of Acanthamoeba isolated from domestic tap water in Korea. Korean Journal of Parasitology 47: 337-44. (sequences of 5 isolates [KA/WP3, KA/WP4, KA/WP8, KA/WP9, and KA/WP12] not deposited in DNA databases, but provided by original investigators).  

Cholewinski M, Solarczyk P, Derda M, Wojtkowiak-Giera A, Hadas E.  2015.  Presence of potential pathogenic genotypes of free-living amoebae isolated from sandboxes in children’s playgrounds.  Folia Parasitol (Praha). 2015 vol 62. p:064.  (accession # KR259807 – KR259821)

Chusattayanond, A., Boonmee, A., Boonsilp, S., Warit, S., Smittipat, N. and Juthayothin,T. 2015.  Genotyping of clinical and soil Acanthamoebae from 22 provinces of Thailand.  Unpublished.  (accession # KF733218 – KF733263,  KF873021,  KF956709, KF956710, KF956711)

Clarke, M., Lohan, A.J., Liu, B., Lagkouvardos, I., Roy, S., Zafar, N., Bertelli, C., Schilde, C., Kianianmomeni, A., Burglin, T.R., Frech, C., Turcotte, B., Kopec, K.O., Synnott, J.M., Choo, C., Paponov, I., Finkler, A., Soon Heng Tan, C., Hutchins, A.P., Weinmeier, T., Rattei, T., Chu, J.S., Gimenez, G., Irimia, M., Rigden, D.J., Fitzpatrick, D.A., Lorenzo-Morales, J., Bateman, A., Chiu, C.H., Tang, P., Hegemann, P., Fromm, H., Raoult, D., Greub, G., Miranda-Saavedra, D., Chen, N., Nash, P., Ginger, M.L., Horn, M., Schaap, P., Caler, L. and Loftus, B.  2013. Genome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of tyrosine kinase signaling. Genome Biol. 14 (2), R11.  (Accession # AEYA01002259)

Cometa, I., Schatz, S., Trzyna, W. and Rogerson, A. 2011.  Tolerance of naked amoebae to low oxygen levels with an emphasis on the genus Acanthamoeba. Acta Protozoologica 50: 33-40.  (accession # HQ637490 – HQ637491). 

Connelly, L., Anijeet, D., Tole, D. and Alexander, C.L., 2023. Acanthamoeba keratitis: Molecular typing of Acanthamoeba species directly from ocular tissue. Current Research in Parasitology & Vector-Borne Diseases, 4, p.100141.  (acc #  MT944202 – MT944213; OR257994 – OR257996

Conza, L. 2011. unpublished. (accession # JF508857).

Conza,L., Casati,S. and Gaia,V. 2013. Influence of climate and geography on the occurrence of Legionella and amoebae in composting facilities. Unpublished. (accession # KC346958 – KC346960).

Conza, L., Pagani, S.C., Gaia, V. 2013. Presence of Legionella and Free-Living Amoebae in Composts and Bioaerosols from Composting Facilities. PLOS ONE  8: e68244 .  (accession #JX983592; KC164218 – KC164253; KC210134 – KC210144). 

Corsaro,D., Feroldi,V., Saucedo,G., Ribas,F., Loret,J.F. and Greub,G. 2009. Novel Chlamydiales strains isolated from a water treatment plant. Environmental Microbiology 11: 188–200. (accession #’s EU074225  – EU707854). 

Corsaro D, Pages GS, Catalan V, Loret JF, Greub G. 2010.  Biodiversity of amoebae and amoeba-associated bacteria in water treatment plants.  International Journal of Hygiene and Environmental Health. 213: 158-66. (accession #’s  FJ627999; FJ628002 – FJ628004).   

Corsaro, D. and Venditti, D. 2010. Phylogenetic evidence for a new genotype of Acanthamoeba (Amoebozoa, Acanthamoebida). Parasitology Research 107 (1), 233-238. (accession # GQ380408).  

Corsaro,D., Walochnik,J., Kohsler,M. and Rott,M.B.  2015.
Acanthamoeba misidentification and multiple labels: redefining genotypes T16, T19, and T20 and proposal for Acanthamoeba micheli. sp. nov. (genotype T19).   Parasitol. Res. 114: 2481–2490.  (acc # KP711387).

Corsaro,D., Kohsler,M., Montalbano Di Filippo,M., Venditti,D., Monno,R., Di Cave,D., Berrilli,F. and Walochnik,J. 2017. Update on Acanthamoeba jacobsi genotype T15, including full-length 18S rDNA molecular phylogeny. Parasitol. Res. 116: 1273-1284.  (acc # KY513789 – KY513796).

Corsaro, D., Kohsler, M., Wylezich, C., Venditti, D., Walochnik, J. and Michel, R. 2017. New insights from molecular phylogenetics of amoebophagous fungi (Zoopagomycota, Zoopagales).  Parasitology Research 117(1):157-167.  (Accession # KY934458 – KY934460).

Corsaro, D., Kohsler, M., Venditti, D., Rott, M.B. and Walochnik, J. 2019. Recovery of an Acanthamoeba strain with two group I introns in the nuclear 18S rRNA gene. Eur. J. Protistol. 68, 88-98.  (acc # MK248592).

Coskun, KA , Ozcelik, S, Tutar, L, , Elaldi, N,  Tutar, Y. 2013. Isolation and Identification of Free-Living Amoebae from Tap Water in Sivas, Turkey. Biomed Research International 2013: Article Number: 675145.  (sequences not deposited). 

Coulon,C., Collignon,A., McDonnell,G. and Thomas,V. 2010. Resistance of acanthamoeba cysts to disinfection treatments used in health care settings. Journal of Clinical Microbiology 48: 2689-2697. (accession # GU459317 – GU459322).  

Crary,M.J., Hopkins,A., Kelly,D., Booton,G.C. and Fuerst,P.A 2013. Ohio River Basin Acanthamoeba isolates exhibit high levels of genetic diversity and environmental tolerance. Unpublished. (accession # JQ669656 – JQ669661).

Cruz, A.R.S., W. L. Rivera. 2014. Genotype analysis of Acanthamoeba isolated from human nasal swabs in the Philippines.   Asian Pacific Journal of Tropical Biomedicine  4(10): 827-831.  (accession # KJ652982 – KJ652990). 

Dadimoghddam, Y., Sarvi, S., Sharif, M. and Daryanii, A. 2017. Isolation and Genotyping of Free Living Amoeba from Soil in Mazandaran Provenance. Unpublished. Medical Parasitology, Mazandaran Medical University, Farah Abad, Sari, Mazandaran, Iran. (accession # MG722753 MG890344, MG890345, MG969816-MG969818, MG969830-MG969831, MG9699630).

Dadimoghdam, Y., Sarvi, S., Sharif, M. and Daryani, A. 2018. Isolation and genotyping of Acanthamoeba and Hartmannella from soil in Mazandaran Provenance at 2017. 2018. Unpublished; Medical Parasitology, Mazandaran University of Medical Sciences (MAZUMS), Mazandaran, Iran.   (accession #’s MG890344 – MG890344)

Damon, C., Lehembre, F., Oger-Desfeux, C., Luis, P., Ranger, J., Fraissinet-Tachet, L., Marmeisse, R. 2012.  Metatranscriptomics Reveals the Diversity of Genes Expressed by Eukaryotes in Forest Soils. PLoS One. 2012; 7(1): e28967.  [accession #s FN394737; FN394824]. 

Da Fonseca, J.D.G., Gomez-Hernandez, C., Barbosa, C.G. and Rezende-Oliveira, K. 2019. Identification of free-living amoebas in the air conditioning systems from a public hospital of Ituiutaba city – MG. Unpublished; Parasitology, Triangulo Mineiro Federal University, Uberaba, Minas Gerais, Brazil. (accession # MK192683 – MK192688).

De Jonckheere, J. F. . 2003. Epidemiological Typing of Acanthamoeba Strains Isolated from Keratitis Cases in Belgium. Bull. Soc. Belge Ophtalmol., 287, 27-33, 2003. (sequences undeposited). 

Delafont,V., Mougari,F., Cambau,E., Joyeux,M., Bouchon,D., Hechard,Y. and Moulin,L. 2014.  First evidence of amoebae-mycobacteria association in drinking water network. Environ. Sci. Technol. 48 (20), 11872-11882. Accession #  KM220682,  KM220688, KM220694, KM220696, KM220712,  KM220719, KM220720, KM220722).

Delafont, V., Samba-Louaka, A., Bouchon, D., Moulin, L. and Hechard, Y.  2015.  Free-living amoebae isolated from Paris drinking water network. Unpublished.  (accession # KT290033).

Del Chierico, F., Di Cave, D., Accardi, C., Santoro, M., Masotti, A., D’Alfonso, R., Berrilli, F., Urbani, A. and Putignani, L. 2016. Identification and typing of free-living Acanthamoeba spp. by MALDI-TOF MS Biotyper. Experimental Parasitology 170: 82-89.  (accession # KX184268 – KX184269; KX682341 – KX682342).

Dendana, F., Sellami, H., Trabelsi, H., Neiji, S., Cheikhrouhou,F., Makni,F. and Ayadi,A. 2013. Acanthamoeba T4 genotypes associated with keratitis infections in Tunisia. Parasitol Res (2013) 112:401–405. (accession # JX009197 – JX009201). 

Derda,M., Solarczyk,P., Cholewinski,M. and Hadas,E. 2015. Genotypic characterization of amoeba isolated from Acanthamoeba keratitis in Poland. Parasitology Research 114 (3), 1233-1237. (accession #’s: KP120879-KP120880; KP184479-KP184485).

de Sousa Mendes Moreira Alves, D., Souza Goncalves, G., Silva Moraes, A., Rodrigues do Carmo Neto, J., Machado Hecht, M., Nitz, N., de Castro, A.M., Chalita, M.R. and Claire Vinaud, M. 2017. Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás (UFG), Goiania, Brazil. Direct Submission.   (accession #’s MF164153 – MF164154).

Di Cave D, Monno R, Bottalico P, Guerriero S, D’Amelio S, D’Orazi C, Berrilli F. 2009.  Acanthamoeba T4 and T15 genotypes associated with keratitis infections in Italy.  European Journal of Clinical Microbiology and Infectious Diseases 28: 607-12.  (accession #’s EF654665 – EF654667; EU741250 – EU741257; FJ195367 – FJ195369).  

Di Cave, D., D’Alfonso, R., D’Orazi, C., Comlavi, K.D., Monno, R. and Berrilli, F.  2014. Genotypic heterogeneity based on 18S rRNA gene sequences among Acanthamoeba isolates recovered from clinical samples in Italy. Unpublished. (accession # KJ094639 – KJ094693).

Dobrowsky, P.H., S. Khan, T.E. Cloete and W. Khan. 2016. Molecular detection of Acanthamoeba spp., Naegleria fowleri and Vermamoeba (Hartmannella) vermiformis as vectors for Legionella spp. in untreated and solar pasteurized harvested rainwater. Parasites & Vectors 9: 539.  (three sequences T4, T5 and T15, undeposited).

Dogan, S., Yazar, S. and Kuk, S.  Acanthamoeba in Turkey. unpublished. (accession #’s JQ315200 – JQ315202).

Dodangeh, S., Kialashaki, E., Daryani, A., Sharif, M., Sarvi, S., Moghaddam, Y.D. and Hosseini, S.A. 2018. Isolation and molecular identification of Acanthamoeba spp. from hot springs in Mazandaran province, northern Iran. J Water Health. 2018 Oct;16(5):807-813.  (accession #’s MG890336; MG890338; MG890617 – MG890619;  MG890629; MG890631; MG891744; MG906986 – MG90698).

dos Santos Gomes, T., Magnet, A., Izquierdo, F., Vaccaro, L., Redondo, F., Bueno, S., Sánchez, M.L., Angulo, S., Fenoy, S., Hurtado, C., and del Aguila, C. 2016. Acanthamoeba spp. in Contact Lenses from Healthy Individuals from Madrid, Spain. PLoS ONE 11(4): e0154246. (one T4 isolate, not deposited).

Duarte, J.L., Furst, C., Klisiowicz, D.R., Klassen, G. and Costa, A.O. 2013 . Morphological, genotypic, and physiological characterization of Acanthamoeba isolates from keratitis patients and the domestic environment in Vitoria, Espirito Santo, Brazil. Experimental Parasitology 135: 9-14.  (accession # JX441872 – JX441878). 

Dupuy,M., Mazoua,S., Berne,F., Bodet,C., Garrec,N., Herbelin,P., Menard-Szczebara,F., Oberti,S., Rodier,M.H., Soreau,S., Wallet,F. and Hechard,Y. 2011. Efficiency of water disinfectants against Legionella pneumophila and Acanthamoeba. Water Research 45: 1087-1094.  (accession # GU936482 – GU936484).  

Dykova,I., Lom,J., Schroeder-Diedrich,J.M., Booton,G.B. and Byers,T.J.  1999.  Acanthamoeba strains isolated from organs of freshwater fishes. Journal of Parasitology 85: 1106-13.  (accession #’s AF140711 – AF140721). 

Dykova, I., Kostka, M., Wortberg, F., Nardy, E. and Peckova, H. 2010. New data on aetiology of nodular gill disease in rainbow trout, Oncorhynchus mykiss. Folia Parasitol. 57 (3), 157-163. (accession # HM363624 – HM363632).    

Dykova,I. and Kostka,M.  2015.  Illustrated Guide to Culture Collection of Free-living Amoebae.  Unpublished.  (Accession # JQ271663 – JQ271667).

Edagawa, A., Kimura, A., Kawabuchi-Kurata, T., Kusuhara, Y. and Karanis, P. 2009.  Isolation and genotyping of potentially pathogenic Acanthamoeba and Naegleria species from tap-water sources in Osaka, Japan.  Parasitology Research 105: 1109-1117.  (accession # AB327060 – AB327096).

Eldeek, H.E. 2019. Direct Submission. Department of Medical parasitology, Faculty of Medicine, Assiut University, Assiut, Egypt. (acc #’s MN091379, MN091838, MN091845, MN091854, MN091860).

Ertabaklar, H., Turk, M., Dayanir, V., Ertug, S. and Walochnik, J.  2007. Acanthamoeba keratitis due to Acanthamoeba genotype T4 in a  non-contact-lens wearer in Turkey.  Parasitology Research 100: 241-246.  (accession #’s DQ185605 – DQ185607; DQ264391).

Eslamirad, Z. 2018. Direct Submission. Parasitology and Mycology, Arak University of Medical Sciences, Pardis of Arak University of Medical sciences, Arak, Iran.  (accession # MG066681).

Eslamirad, Z., Ahmadi, A. and Hajihossein, R. 2018. Acanthamoeba from Arak soil. Unpublished; Parasitology and Mycology, Arak University of Medical Sciences, Arak, Iran.  (accession # MG298785 – MG298794).

Evyapan,G., Koltas,I.S. and Eroglu,F. 2014. Genotyping of Acanthamoeba T3, T4, T15 strains obtained from environment in Turkey. Unpublished. (accession # KJ446975 – KJ446982).

Fallah, E., Jafarpour, Z., Mahami Oskouei, M., Haghighi, A. and Niyyati, M. 2015.  Molecular Identification of Acanthamoeba isolates from water in Tabriz, 2014.  Unpublished.  (accession # KP940443 – KP940446).

Fernandez, M.T., Rubio, E., Alejandre, M.C., Lera, D., Navarro, M. and Goni, P. 2016. Identification of potentially pathogen free-living amoeba in swimming pools in Spain. Unpublished. Microbiology, Preventive Medicine and Public Health, University of Zaragoza, Zaragoza, Spain.   (accession # KY038361 – KY038363).

Fiore-Donno, A.M., Weinert, J., Wubet, T. and Bonkowski, M.  2016.  Metacommunity analysis of amoeboid protists in grassland soils.  Scientific Reports 6: 19068.  (accession # KP075362 – KP075634)

Fucikova, K. and Lahr, D.J.  2015.  Uncovering Cryptic Diversity in Two Amoebozoan Species Using Complete Mitochondrial Genome Sequences. J. Eukaryot. Microbiol. In press.  (acc # KT185626).

Fukumoto, T., Matsuo, J., Okubo, T., Nakamura, S., Miyamoto, K., Oka,K., Takahashi, M., Akizawa, K., Shibuya, H., Shimizu, C. and Yamaguchi, H. 2016. Acanthamoeba containing endosymbiotic chlamydia isolated from hospital environments and its potential role in inflammatory exacerbation. BMC Microbiol. 16: (1), 292. (accession # LC122508 – LC122510).

Fuerst,P.A., Crary,M.J., Booton,G.C., Lares-Villa,F., Shoff,M., Joslin,C., Tu,E. and Pearlman,E. 2013. Legionella and Pseudomonas Associated with the Protist Acanthamoeba during a Keratitis Outbreak. Unpublished. (accession # JX423576 – JX423611).

Fuerst, P.A., Crary, M.J. and Booton, G.C. 2019. Cytochrome C Oxidase Subunit I (COI) Taxon Barcoding of the Opportunistically Pathogenic Protists Acanthamoeba and Balamuthia. Unpublished. (acc # MN232007, MN239984 – MN239993)

Gad, M.A. 2019. Genotyping and interaction-reality of Acanthamoeba and enteric viruses in drinking water, Egypt. Unpublished; Water Pollution Research Department, National Research Centre, El-Buhouth, Dokki, Giza 12622, Egypt. (accession # MK217498 – MK217520).

Gaide, N., Pelandakis, M., Robveille, C., Albaric, O., Jouvion, G., Souchon, M., Risler, A. and Abadie, J. 2015. Necrotizing Meningoencephalitis in a Captive Black and White Ruffed Lemur (Varecia variegata variegata) Caused by Acanthamoeba T4 Genotype.  J. Comp. Pathol. 153(4):231-5.  (Accession # KM434770).

Gavarāne, I, Trofimova, J, Mališevs, A, Valciņa, O, Kirjušina, M, Rubeniņa, I, Bērzinš, A. 2018. DNA extraction from amoebal isolates and genotype determination of Acanthamoeba from tap water in Latvia Parasitol. Res. Parasitol Res.  117(10): 3299-3303.  (Accession # MG386295 – MG386314).

Garcia, A., Goni, P., Clavel, A., Ormad, M.A.P. and Lobez, S. 2011. Potentially pathogenic free-living amoebae (FLA) isolated in Spanish wastewater treatment plants. Environmental Microbiology Reports  3: 622–626.  (accession # HQ007036 – HQ007042). 

Garcia, A., Goni, P., Cieloszyk, J., Fernandez, M.T., Calvo-Begueria, L., Rubio, E., Fillat, M.F., Peleato, M.L. and Clavel, A.  2013.  Identification of free-living amoebae and amoeba-associated  bacteria from reservoirs and water treatment plants by molecular techniques. Environ. Sci. Technol. 47 (7), 3132-3140.  (accession # JQ678604 – JQ678671).   

Garcia, A. and Goni, P. 2013. Bioko Island, Equatorial Guinea study of pathogen amoebae in water and related human stool samples. Unpublished.  (accession # KF433819 – KF433826).

Garcia-Sanchez, A.M., Ariza, C., Ubeda, J.M., Martin-Sanchez, P.M., Jurado, V., Bastian, F., Alabouvette, C., Saiz-Jimenez, C. 2013. Free-living amoebae in sediments from the Lascaux Cave in France.   International Journal of Speleology 42: 9-13.  (accession #HE653911 – HE653914). 

Garrido, K., Ileto, A., De Jesus, V., Emperador, W., Francisco, A., Garcia, P., Hadap, A., Hernandez, M.E., Lacson, J.F., Lagudas, C.R. and Padua, M.F., 2023. Occurrence of Acanthamoeba spp. in a major river in the Philippines: Impact on water quality and health. Journal of Water, Sanitation and Hygiene for Development, 13(11), pp.885-892. (acc # OR447489, OR457669, OR457670, OR461515)

Garcia Berrios, L.R., Lopez Guinea, C.L. and Leiva Torres, B. 2018. Frequency of free living amoeba in water sources of the Espana Hospital of the city of Chinandega in the period from May to June of 2012. Unpublished; Parasitology, School of Microbiology, Universidad de Costa Rica, Ciudad Universitaria Rodrigo Facio, San Jose, Costa Rica.   (accession # MH824158.

Gast, R.J. (2001) Development of an Acanthamoeba-specific reverse dot-blot and the discovery of a new ribotype.  Journal of Eukaryotic Microbiology 48: 609-615. (accession #AF333607 – AF333609; plus 11 isolates typed without sequences provided).  

Gast,R.J., Ledee,D.R., Fuerst,P.A. and Byers,T.J.  1996.  Subgenus systematics of Acanthamoeba: four nuclear 18S rDNA sequence types.  Journal of Eukaryotic Microbiol. 43 (6), 498-504.  (accession #’s U07400 – U07417). 

Gast, R.J., Moran, D.M., Dennett, M.R., Wurtsbaugh, W. and Amaral-Zettler, L. 2011.  Amoebae and Legionella pneumophila in coastal saline environments. Journal of Water and Health 9:  37-52.  (accession #’s GU320576 – GU320610).   

Gast, R.J., Byers, T.J. and Fuerst, P.A. 2017. 18S rRNA gene sequences from isolates of Acanthamoeba associated with keratitis. Unpublished. Ohio State University, Columbus, Ohio, USA  (Ten sequences not deposited).

Gatti, S., Rama, P., Matuska, S., Berrilli, F., Cavallero, A., Carletti, S., Bruno, A., Maserati, R., and Di Cave, D.  2010. Isolation and genotyping of Acanthamoeba strains from corneal infections in Italy. Journal of Medical Microbiology 59, 1324-1330. (Accession # KT735324 – KT735338)

Geisen,S., Fiore-Donno,A.M., Walochnik,J. and Bonkowski,M.  2014.  Acanthamoeba everywhere: high diversity of Acanthamoeba in soils. Parasitol. Res. 113(9):3151-8. (accession # KF928933 – KF928956).

Ghaderifar, S., Najafpoor, A.A., Zarrinfar, H., Esmaily, H. and Hajialilo, E. 2018. Isolation and identification of Acanthamoeba from pond water of parks in a tropical and subtropical region in the Middle East, and its relation with physicochemical parameters. BMC Microbiology (2018) 18:139.   (accession #  MF944085 – MF944088).

Ghamilouie, M.M., Valadkhani, Z., Rahimi, F., Khoshzaban, F., Aghighi, Z. and Hassan, N. 2018. Isolation and Genotyping of Acanthamoeba Strains from Corneal Scraps. Iran J Ophthalmol 26 (2), 97-101 .  (acc # MG917703 – MG917708).

Ghasemi,A.A., Babaei,Z., Ziaali,N., Rostami,S. and Fasihi. 2013. Acanthamoeba genotypes, 18S ribosomal RNA gene, partial sequence, from environmental samples in Kerman,Iran. Unpublished.   (accession # KC694178 – KC694193).

Gianinazzi,C., Schild,M., Wuthrich,F., Ben Nouir,N., Fuchslin,H.-P., Schurch,N., Gottstein,B. and Muller,N. 2009. Screening Swiss water bodies for potentially pathogenic free-living amoebae. Research in Microbiology 160: 367-374. (accession #’s  FJ940706 – FJ948857).    

Gianinazzi, C., Schild, M.,  Fritz Wüthrich, F,.  Müller, N., Schürch, N., Gottstein, B., 2009.  Potentially human pathogenic Acanthamoeba isolated from a heated indoor swimming pool in Switzerland. Experimental Parasitology 121: 180–186. (accession # not deposited).

Gianinazzi,C., Schild,M., Zumkehr,B., Wuthrich,F., Nuesch,I., Ryter,R., Schurch,N., Gottstein,B. and Muller,N. 2010. Screening of Swiss hot spring resorts for potentially pathogenic free-living amoebae. Experimental Parasitology 126:  45-53. (accession #’s GU016571; GQ996530 – GQ996537; GU199433 – GU199437).   

Glucksman,E., Snell,E.A., Berney,C., Chao,E.E., Bass,D. and Cavalier-Smith,T. 2011. The novel marine gliding zooflagellate genus Mantamonas (Mantamonadida ord. n.: Apusozoa). Protist 162 (2), 207-221. (accession #GU001160).  

Golestani, M.H., Rasti, S., Hooshyar, H. and Delavari, M. 2016. Acanthamoeba genotypes from hospital dust in Isfahan, Kashan, Iran. unpublished. (accession # LC184513 – LC184519; LC183867)

Goux, X., Calusinska, M., Fossepre, M., Benizri, E. and Delfosse, P. 2016.
Start-up phase of an anaerobic full-scale farm reactor – Appearance of mesophilic anaerobic conditions and establishment of the methanogenic microbial community. Bioresource Technology 212: 217-226.  (accession # KT386430, KT386471, KT386478, KT386612, KT386640, KT386655, KT386668, KT386772, KT386845).

Gonzalez-Robles,A., Salazar-Villatoro,L., Omana-Molina,M., Reyes-Batlle,M., Martin-Navarro,C.M. and Lorenzo-Morales,J.  2014. Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical Case.  J Parasitol Res 2014, 256310.   (accession #KF914142).

Greninger, A.L., Jerome, K. and Dixon, T. 2016. Genome sequence of Acanthamoeba castellani mitochondria. Unpublished. Laboratory Medicine, UW, Seattle, WA, USA. (two sequences not deposited).

Greub, G., Drancourt, M. and Raoult, D. 2003.  Broad-range PCR targeting the 18S rRNA gene for the identification of protists isolated from hospital water network. Unpublished.  (accession #’s AY237734 – AY237735).  [See Thomas et al. below].

Grun, AL, Stemplewitz, B., Scheid, P. 2014.  First report of an Acanthamoeba genotype T13 isolate as etiological agent of a keratitis in humans. Parasitology Research 113: 2395-2400. (accession # KJ476522).

Gunderson, J.H. and Sogin, M.L. 1986. Length variation in eukaryotic rRNAs: Small subunit rRNAs from the protists Acanthamoeba castellanii and Euglena gracilisGene 44: 63-70.  (accession # M13435). 

Habibpour, H. 2018. Molecular identification and sequencing of waterborne free-living amoeba (Acanthamoeba, Naegleria and Vermamoeba) isolated from unimproved hot springs, Guilan Province, Northern Iran. Unpublished; Parasitology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Golestan, Ahvaz, Khuzestan, Iran.  (accession # MH347242  – MH347253).

Habibpour, H. 2019. Direct Submission; Parasitology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61357-15794, Iran. (accession # MK422924, MK441743, MK441744).

Hadas, E., Cholewinski, M., Derda, M. and Solarczyk, P. 2016. Direct Submission. Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Poznan, Poland. (accession # KY203908) .

Hajialilo, E., Niyyati, M., Solaymani, M. and Rezaeian, M.  2015.  Pathogenic Free-Living Amoebae Isolated From Contact Lenses of Keratitis Patients. Iran J Parasitol 10: 541-546.  (accession # KP714398).

Hajialilo, E., Behnia, M., Tarighi, F., Niyyati, M. and Rezaeian, M. 2016. Isolation and genotyping of Acanthamoeba strains (T4, T9, and T11) from amoebic keratitis patients in Iran. Parasitology Research 115: 3147-3151.  (accession # KU936102 – KU936119).

Hajialilo, E., Paknejad, N. and Saraei, M. 2018. Genotyping of waterborn free living amoebae isolated from public swimming pools in Qazvin, Iran. Unpublished; Dept of Parasitology, Qazvin University of Medical Sciences, Qazvin, Iran. (accession # MH024474MH024482 ; MH024491 – MH024492).

Heinz, E., Kolarov, I., Kastner, C., Toenshoff, E.R., Wagner, M. and Horn, M. 2007.  An Acanthamoeba sp. containing two phylogenetically different bacterial endosymbionts. Environmental Microbiology 9: 1604-1609.  (accession # AM412762). 

Heredero-Bermejo, I., Martin, C.S., de Carranza, J.S., Copa-Patino, J.L., Perez-Serrano, J.  2012.  Acanthamoeba castellanii: in vitro UAH-T17c3 trophozoite growth study in different culture media. Parasitology Research  110: 2563-2567.  (accession # JF437606). 

Heredero-Bermejo,I., Criado-Fornelio,A., DE Fuentes,I., Soliveri,J., Copa-Patino,J.L. and Perez-Serrano,J. 2014. Characterization of a human-pathogenic Acanthamoeba griffini isolated from a contact lens-wearing keratitis patient in Spain.  Parasitology, 1-11 (2014) In press (accession # KF010846).

Hertz-Fowler, C., Noyes, H., Brenchley, R., Goodhead, I., Hall, N. and D’Amore, R. 2015.  Phylogenomics of Acanthamoeba species: The Acanthamoeba whole genome shotgun (WGS) project.  Direct Submission.  Unpublished.  Centre for Genomic Research,  Institute of Integrative Biology,  University of Liverpool,  United Kingdom.  Bioproject Accession: PRJEB7687

Hewett, M.K. 2006.  Characterisation of Bacterial Symbionts in Amoebae. PhD Thesis (2006) University of South Australia and Australian Water Quality Centre, Boliver, SA, Australia.  (accession #’s AY549557 – AY549562). 

Hewett, M.K., Robinson, B.S., Monis, P.T. and Saint, C.P. 2003.  Identification of a new Acanthamoeba 18S rRNA gene sequence type, corresponding to the species Acanthamoeba jacobsi Sawyer, Nerad and Visvesvara, 1992 (Lobosea: Acanthamoebidae).  Acta Protozoologica 42: 325-329.  (accession#’s AY262360 – AY262365). 

Hooshyar, H., Aghajani, A., Dabirzaedh, M. and Maroufi, Y. 2015. Acanthamoeba 18S ribosomal RNA genes. Unpublished.  (accession # LC085656, LC085657, LC085894, LC085971 – LC085973,  LC086294 – LC086296)

Hooshyar, H. and Aghajani, A.  2015.  Acanthamoeba genotypes  –  Kashan University of Medical Sciences, Qotbe Ravandi, Kashan, Isfahan 0361, Iran. Unpublished.  (accession #’s LC093098, LC093126, LC093460, LC093461, LC093499, LC093844, LC094346, LC094422, LC094962, LC095808, LC096083, LC101790, LC101791).

Hooshyar,H.  2016.  Acanthamoeba genotypes  –  Kashan University of Medical Sciences, Qotbe Ravandi, Kashan, Isfahan 0361, Iran.  Unpublished. (accession # LC106354).

Horn, M., Fritsche, T.R., Gautom, R.K., Schleifer, K.H. and Wagner, M.  1999.  Novel bacterial endosymbionts of Acanthamoeba spp. related to the Paramecium caudatum symbiont Caedibacter caryophilus.   Environmental Microbiology 1: 357-367.  (accession #AF132134 – AF132136). 

Horn, M., Fritsche,T.R., Linner, T., Gautom, R.K., Harzenetter, M.D. and Wagner, M. 2002.  Obligate bacterial endosymbionts of Acanthamoeba spp. related to the beta-Proteobacteria: proposal of ‘Candidatus Procabacter acanthamoebae’ gen. nov., sp. nov.  International Journal of Systematic and  Evolutionary Microbiology 52: 599-605.  (accession #’s AF352387 – AF352391;  AM941721). 

Horn, M., Harzenetter,M.D., Linner, T., Schmid,E.N., Muller, K.D., Michel, R. and Wagner, M. 2001. Members of the Cytophaga-Flavobacterium-Bacteroides phylum as intracellular bacteria of acanthamoebae: proposal of ‘Candidatus Amoebophilus asiaticus’. Environmental Microbiology 3: 440-449.  (accession # AF366580). 

Hoshino,Y.T. and Matsumoto,N.  2007.  Changes in fungal community structure in bulk soil and spinach rhizosphere soil after chemical fumigation as revealed by 18S rDNA PCR-DGGE.  Soil Science and Plant Nutrition. 53, 40-55. (accession # AB262828).

Hsu, B.-M., Huang, C.C.,  Chen, J-S., Chen, N.H., and Huang, J-T.  2011. Comparison of potentially pathogenic free-living amoeba hosts by Legionella spp. in substrate-associated biofilms and floating biofilms from spring environments.  Water Research 45: 5171-5183.  (Accession #’s GU553135; GU569844; GU573853 – GU573872; GU596994 – GU597018). 

Hsu, B.-M., Hsu, Y.-W. and Chen, J.-S. 2019. Comparative evaluation of PCR-based methods for the detection of Acanthamoeba by a small-volume collection in aquatic environments: High Acanthamoeba prevalence with genotype identified using improved nested PCR. Unpublished; Department of Earth and Environmental Sciences, National Chung Cheng University, Minhsiung, Chiayi, Taiwan, R.O.C. (Accession # MK390840 – MK390876).

Huang, S.W. and Hsu, B.M.. 2010. Isolation and identification of Acanthamoeba from Taiwan spring recreation areas using culture enrichment combined with PCR. Acta Tropica 115:  282-287. (accession #’s GU573873 – GU573885) 

Hussain, R.H.M., , Anuar, T.S., Ghani, M.K.A. and Khan, N.A. 2019. Isolation and genotyping of Acanthamoeba isolated from contact lens paraphernalia in Peninsular Malaysia. Unpublished; Centre of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam Campus, Kuala Selangor, Selangor, Malaysia. (acc #’s MH791017 – MH791025).

Hussain, R.H.M., Ishak, A.R., Ghani, M.K.A., Khan, N.A., Siddiqui, R. and Anuar, T.S. 2019.  Occurrence and molecular characterisation of Acanthamoeba isolated from recreational hot springs in Malaysia: evidence of pathogenic potential. J Water Health wh2019214. (Accession # MH790980 – MH791016).

Ithoi I., Mahmud R., Abdul Basher M.H., Jali A., Abdulsalam A.M., Ibrahim J., Mak J.W.. 2013. Acanthamoeba genotype T4 detected in naturally-infected feline corneas found to be in homology with those causing human keratitis. Trop Biomed. 30(1):131-40. (Accession # JX494391 – JX494400). 

Javanmard, E., Niyyati, M., Lorenzo-Morales, J., Lasjerdi, Z., Behniafar, H. and Mirjalili, H. 2017. Molecular identification of waterborn free living amoebae (Acanthamoeba, Naegleria and Vermamoeba) isolated from municipal drinking water and environmental sources, north half of Iran. Experimental Parasitology 183:240-244.  (Accession # MF462196)

Jeong HJ, Lee SJ, Kim JH, Xuan YH, Lee KH, Park SK, Choi SH, Chung DI, Kong HH, Ock MS, Yu HS. 2007. Acanthamoeba: keratopathogenicity of isolates from domestic tap water in Korea.  Experimental Parasitology 2007; 117: 357-367.  [20 isolates sequenced but not deposited; phylogenetic tree allows genotype identification] 

Jercic, M.I., Aguayo, C., Saldarriaga-Córdoba, M., Muiño, L., Chenet, S.M., Lagos, J., Osuna, A. and Fernandez, J. 2019. Genotypic diversity of Acanthamoeba strains isolated from Chilean patients with Acanthamoeba keratitis.   Parasites & Vectors 2019 12:58.  (Accession #JF702872 – JF702914 and accession # KX688012 – KX688040 , MH100805 – MH100849).

Ji, W.-T., Hsu, B-M., Chang, T.-Y., Hsu, T.-K., Kao, P.-M., Huang, K.-H., Tsai, S.-F., Huang, Y.-L., Fan, C.-W. 2014. Surveillance and evaluation of the infection risk of free-living amoebae and Legionella in different aquatic environments. Science of the Total Environment 499: 212–219. (accession # KY245937 – KY245937).

Jiang,C., Sun,X.G., Wang,Z.Q. and Zhang,Y.  2014.  Genotyping of 102 Acanthamoeba isolates from patients with Acanthamoeba keratitis in China. Unpublished. (accession # KJ631421 – KJ631522).

Johnson, A.M., Fielke, R., Christy, P.E., Robinson, B. and Baverstock, P.R. 1990.  Small subunit ribosomal RNA evolution in the genus AcanthamoebaJournal of General Microbiology 136:  1689-1698.  (accession #’s  M57865 – M57885).   

Juarez, M.M., Sanguino Jorquera, D.G., Poma, H.R., Irazusta, V. and Rajal, V.B. 2017. Pathogenic Acanthamoeba in recreational water in Salta, Argentina. Laboratorio de Aguas y Suelos, INIQUI, Instituto de Investigaciones para la Industria Quimica, Salta, Argentina. Unpublished. (accession #’s KY751406 – KY751414).

Kao,P.-M., Hsu,B.-M., Chen,C.-T., Huang,S.-W., Kao,E.-S., Chen,J.-L., Wu,N.-M. and Ji,W.-T. 2014. Identification and quantification of the Acanthamoeba species and genotypes from reservoirs in Taiwan by molecular techniques. Acta Tropica 132: 45-50. (accession # KF881878 – KF881889). 

Kao, P-M., B-M. Hsu, T-K. Hsu, J-H. Liu, H-Y. Chang, W-T. Ji, K-J. Tzeng, S-W. Huang and Y-L. Huang.  2014.  Seasonal distribution of potentially pathogenic Acanthamoeba species from drinking water reservoirs in Taiwan.  Environmental Science and Pollution Research 22:3766–3773.  (accession #’s KY245928KY245932,  plus 30 undeposited sequences identified by similarity to deposited isolates and 5 undeposited sequences with unidentified similarity).

Karakus, M., Aykur, M., Dagci, H., Ozbel, Y. and Toz, S.  2016.  Molecular Detection and Genotyping of Acanthamoeba spp. among stray dogs using Conjunctival Swab Sampling.  Acta Tropica 164: 23–26 .  (accession # KX018021 – KX018047). 

Karcs, C.C., Mears, M.C., Warang, S.S., Capps, H.D., Eremeeva, M.E. and Aslan, A. 2018. Detection of Free-Living Amoeba and Amoeba Resisting Bacteria in Southeastern Georgia. Unpublished. Environmental Health Sciences, Georgia Southern University, Statesboro, GA, USA.  (Accession # MG564162)

Karlyshev, A.V. 2016. Genome sequencing of A. polyphaga Linc Ap-1. Unpublished. Faculty of Science, Engineering and Computing, Kingston University, Kingston upon Thames, United Kingdom.  (Accession # LQHA01000000).

Kashiwabuchi RT, Carvalho FR, Khan YA, de Freitas D, Foronda AS, Hirai FE, Campos MS, McDonnell PJ.  2011. Assessing efficacy of combined riboflavin and UV-A light (365 nm) treatment of Acanthamoeba trophozoites. Investigative Ophthalmology and Visual Sciences 52: 9333-9338.  (accession # JN222978).

Kent, M., Platt, S.R., Rech, R., Eagleson, J.S., Howerth, E.W., Shoff, M.E., Fuerst, P.A., Booton, G.C., Visvesvara, G.S., and Schatzberg, S.J. 2011. Multisystemic infection with an Acanthamoeba sp in a dog. Journal of the American Veterinary Medical Association 238: 1476-1481.(accession # GQ924681 – GQ924682).  

Khademvatan, S., Abdi, M., Diba, K., Hazrati, K. and Niyyati, M. 2018. Free Living Amoeba in water sources of Urmia North west Iran. Unpublished; School of Medicine, Cellular and Molecular Research Center, Department of Parasitology; Urmia, West Azarbayjan, Iran.  (accession # LC426995 – LC427004).

Khan, N.A., Jarroll, E.L., Lunt, D.H. and Paget, T.A. 2002.  Molecular and physiological differentiation between pathogenic and nonpathogenic AcanthamoebaCurrent Microbiology 45: 197-202.   (accession #’s AF239162AF239293 – AF239305).   

Khatonaki, H. and Solhjou, K. 2018. Investigation of Frequency of dental units water contaminated with Acanthamoeba species by morphological and molecular techniques in Jahrom in 2017. Unpublished; Medical Parasitology, Jahrom University of Medical Sciences, Motahari, Jahrom, Shiraz, Iran.  (accession # MG581703, MG581704, MG581707, MG581709, MG581710.

Khosravinia, N., Fata, A., Eslampour, A., Sedaghat, M.R. and Hosseini Farash, B.R. 2019. Direct Submission. Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Khorasan, Iran. (accession #MK182302)

Khosravinia, N., Moghaddas, E., Fata, A., Eslampour, A., Hosseini Farash, B.R. and Sedaghat, M.R. 2019. Direct Submission. Department of Parasitology and Mycology, Faculty of Medicine, Bahonar, Mashhad, Khorasan, Iran. (acc # MK297910 – MK297918)

Kilic, A.,  Tanyuksel,  M.,  Sissons, J., Jayasekera,  S., and Khan,  N.A. 2004. Isolation of Acanthamoeba isolates belonging to T2, T3, T4, and T7 genotypes from environmental samples in Ankara, Turkey.  Acta Parasitologica  2004; 49 (3): 246–252.  (sequences not deposited).

Kinde, H., Read, D.H., Daft, B.M., Manzer, M., Nordhaussen, R.W., Vrono, D.S., Booton, G.C., Kelly, D.J., Fuerst, P.A. and Visvesvara, G.S.  2007. Infections caused by pathogenic free-living amoebae (Balamuthia mandrillaris and Acanthamoeba sp.) in horses.  Journal of Veterinary Diagnostic Investigation 19: 317-322. (accession # DQ499151).   

Kiss C., Barna Z., Vargha, M., Török, J.K.  2014. Incidence and molecular diversity of Acanthamoeba species isolated from public baths in Hungary. Parasitol Res. 113: 2551-2557.  (accession # HG797013 – HG797021).

König, L., Wentrup, C., Schulz, F., Wascher, F., Escolar, S., Swanson, M.S., Buchrieser, C. and Horn, M. 2019. Symbiont-mediated defense against Legionella pneumophila in amoebae. mBio10:e00333-19. (acc # MH675534).

Kohsler, M., Leitner, B., Blaschitz, M., Michel, R., Aspock, H. and Walochnik, J.  2006.  ITS1 sequence variabilities correlate with 18S rDNA sequence types in the genus Acanthamoeba (Protozoa: Amoebozoa).  Parasitology Research 98: 86-93.  (accession #’s  AY661856 – AY661860). 

Kohsler, M., Leitsch, D., Furnkranz, U., Duchene, M., Aspock, H. and Walochnik, J. 2008.  Acanthamoeba strains lose their abilities to encyst synchronously upon prolonged axenic culture. Parasitology Research 102: 1069-1072. (accession #’s EF429130, EF429131, EF554328).   

Koltas, IS, F. Eroglu, E. Erdem, M. Yagmur and F. Tanir. 2015. The role of domestic tap water on Acanthamoeba keratitis in non-contact lens wearers and validation of laboratory methods. Parasitology Research 114:3283–3289. (undeposited sequences).

Koitabashi, M., Noguchi, M.T., Sameshima-Yamashita, Y., Hiradate, S. Suzuki, K., Yoshida, S., Watanabe, T., Shinozaki, Y., Tsushima, S., Kitamoto, H.K.  2012.  Degradation of biodegradable plastic mulch films in soil environment by phylloplane fungi isolated from gramineous plants.  AMB Express. 2012 Aug 2;2(1):40.   Accession # AB727584, AB727588 , AB727589).

Koltas, I.S., Eroglu, F., Erdem, E., Yagmur, M. and Tanir, F.  2015.  The role of domestic tap water on Acanthamoeba keratitis in non-contact lens wearers and validation of laboratory methods. Parasitology Research (2015) In press.  (accession # KR872641 – KR872646, plus 53 additional undeposited isolates identified by similarity to deposited isolates).

Komogorova VV, Sharova NI, Gordeeva LM, Iarilin AA.  2003.  [Acanthamoeba isolated from child thymus: Acanthamoeba growth during cocultivation with human and murine eukaryotic cells and induction of cytopathogenic effect on these cells]. [Article in Russian]  Med Parazitol (Mosk). 2003 Apr-Jun;(2):24-8.. (accession # HM146309)

Koshel’, E.I., Anisimova, L.V., Novichkova, L.A., Vidiaeva, N.A., Guseva, N.P., Eroshenko, G.A., Kutyrev, V.V. 2015. A study on the taxonomy of soil amoebas from Caspian plague foci based on an analysis of ribosomal operon sequences. Genetika. 2015 Jan;51(1):39-45. [Article in Russian; Translation in: Russian Journal of Genetics, 2015, Vol. 51, No. 1, pp. 33–38.]  (accession #’s  MH919367, MH920246, MH920247)

Kusuhara, Y., Kimura, A. and Edagawa, A.  2011. Detection and identification of Acanthamoeba species from dusts of air conditioner filters.  Unpublished. (accession #’s AB594478 – AB594483).

Kuk,S., Yazar, S.,  Dogan, S. , Cetinkaya, U., Sakalar, C. 2013. Molecular characterization of Acanthamoeba isolated from Kayseri well water. Turkish Journal of Medical Sciences 43:  12-17. (accession #  JN793396 – JN793398). 

Lagkouvardos, I., Shen, J. and Horn, M.  2014. Improved axenization method reveals complexity of symbiotic associations between bacteria and acanthamoebae. Environmental Microbiology Reports 6: 383–388. (accession # KF924599 – KF924605). 

Landell MF, Salton J, Caumo K, Broetto L, Rott MB. 2013. Isolation and genotyping of free-living environmental isolates of Acanthamoeba spp. from bromeliads in Southern Brazil. Exp. Parasitol. 134 (3), 290-294.  (accession #’s JX683392 – JX683400). 

Lanocha, N., Kosik-Bogacka, D, Maciejewska, A, Sawczuk, M, Wilk, A.  2009.  The Occurrence Acanthamoeba (Free Living Amoeba) in Environmental and Respiratory Samples in Poland. Acta Protozoologica, 48: 271-279.  (accession #’s GQ342606 – GQ342609).

Laoprasert,T., Kanchanakhan,S., Yagita,K., Wada,S., Kurata,O., Chinabut,S. and Hatai,K. 2007. Molecular characterization of an Acanthamoeba sp. isolated from a naturally-infected oscar, Astronotus ocellatus. Unpublished. (accession # AB561887 ).

Lara, E., Berney, C., Ekelund, F., Harms, H. and Chatzinotas, A. 2007. Molecular comparison of cultivable protozoa from a pristine and a polycyclic aromatic hydrocarbon polluted sit.  Soil Biology and Biochemistry 39 : 139-148. (accession # AY965873). 

Lasjerdi, Z., Niyyati, M., Haghighi, A., Shahabi, S., Biderouni, F.T., Taghipour, N., Eftekhar, M. and Mojarad, E. 2011.  Potentially pathogenic free-living amoebae isolated from hospital wards with immunodeficient patients in Tehran, Iran. Parasitology Research  109: 575-580.  (accession # HQ833407 – HQ833444). 

Lass A, Szostakowska B, Idzin’ska A, Chomicz L. 2014. The first genotype determination of Acanthamoeba potential threat to human health, isolated from natural water reservoirs in Poland. Parasitol Res. 113: 2693 – 2699. (accession # KF924262 – KF924269).

Latifi, A., Salimi, M. and Kazemi, E. 2018. Isolation and identification of free living amoeba from Hot springs and coasts of Ramsar tourist city, in northern Iran. Unpublished; Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. (accession # MH938694 – MH938703).

Leal, D.A.G., Souza, D.S.M., Caumo, K.S., Fongaro, G., Panatieri, L.F., Durigan, M., Rott, M.B., Barardi, C.R.M. and Franco, R.M. 2017. Genotypic characterization and assessment of infectivity of human waterborne pathogens recovered from oysters and estuarine waters in Brazil. Unpublished.   (accession # KY613497 – KY613501).

Lee SJ, Jeong HJ, Lee JE, Lee JS, Xuan YH, Kong HH, Chung DI, Ock MS, Yu HS. 2006. Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear. Korean Journal of Parasitology 44: 313-320. [one isolate sequenced but not deposited; information allows genotype identification]. 

Lesaulnier, C., Papamichail, D., McCorkle, S., Ollivier, B., Skiena, S., Taghavi, S., Zak, D. and van der Lelie, D. 2008. Elevated atmospheric CO2 affects soil microbial diversity associated with trembling aspen. Environmental Microbiology 10: 926-941.  (accession #’s EF023782, EF023500, EF023541, EF023643, EF023659, EF023692, EF023694, EF023745, EF023774, EF024194).

Ledee, D.R., Hay, J., Byers, T.J., Seal, D.V. and Kirkness, C.M.  1996. Acanthamoeba griffini:. Molecular characterization of a new corneal pathogen. Investigative Ophthalmology and Visual Sciences 37: 544-550.  (accession # S81337).

Ledee, D.R.  Seal, D.V., and Byers, T.J. 1998.  Confirmatory Evidence from 18S rRNA Gene Analysis for In Vivo Development of Propamidine Resistance in a Temporal Series of Acanthamoeba Ocular Isolates from a Patient.  Antimicrobial Agents and Chemotherapy, 42: 2144–2145.  (three sequences undeposited).

Ledee,D.R., Iovieno,A., Miller,D., Mandal,N., Diaz,M., Fell,J., Fini,M.E. and Alfonso,E.C. 2009. Molecular identification of T4 and T5 genotypes in isolates from acanthamoeba keratitis patients. Journal of Clinical Microbiology 47: 1458-1462. (accession #’s FJ422510 – FJ422546).   

Levine,M., Block,C. and Hidalgo-Grass,C. 2008. Genotyping of Israeli Acanthamoeba Isolates. Unpublished. (accession #’s FJ042635 – FJ042651).

Li, X.R., Lv, Y,, Meng, H,, Gu, J.D., Quan, Z.X. 2014. Analysis of microbial diversity by pyrosequencing the small-subunit ribosomal RNA without PCR amplification. Appl Microbiol Biotechnol. 8: 3777-89. (accession # GU922825).

Liang,S.-Y., Ji,D.-R., Hsia,K.-T., Hung,C.-C., Sheng,W.-H., Hsu,B.-M., Chen,J.-S., Wu,M.-H., Lai,C.-H. and Ji,D.-D. 2010. Isolation and identification of Acanthamoeba species related to amebic encephalitis and non-pathogenic free-living amoeba species from the rice field. Journal of Applied Microbiology 109 : 1422–1429. (accession # FJ710512 – FJ710514; GU575126).     

Lin, X., Mckinley, J., Resch, C., Kaluzny, R., Lauber, C., Fredrickson, J., Knight, R. and Konopka, A. 2012. Spatial and temporal dynamics of the microbial community in the Hanford unconfined aquifer. ISME Journal 6: 1665-1676. (accession #’s JN705539 – JN705540). 

Liu, H., Ha, Y-R., Lee, S-T. , Hong, Y.-C., Kong, H-H., and Chung, D-I. 2006. Genetic diversity of Acanthamoeba isolates from ocean sediments.  Korean Journal of Parasitology 44: 117–125.  (accession #’s AF005995 – AF005998; AF316545 – AF316547; AF349044 – AF349045; AY148954 – AY148964; AY172999 – AY173015; AY176047). 

Liu, H., Moon, E-K., Yu, H-S., Jeong, H-J., Hong, Y-C.,  Kong, H-H. and Chung, D-I.  2005.  Evaluation of taxonomic validity of four species of Acanthamoeba: A. divionensis, A. paradivionensis, A. mauritaniensis, and A. rhysodes, inferred from molecular analyses. Korean Journal of Parasitology 43: 7–13.  (accession #’s AY351644 – AY351647). 

Lorenzo-Morales, J., Ortega-Rivas, A., Martinez, E., Khoubbane, M., Artigas, P., Periago, M.V., Foronda, P., Abreu-Acosta, N., Valladares, B. and Mas-Coma, S.  2006.  Acanthamoeba isolates belonging to T1, T2, T3, T4 and T7 genotypes from environmental freshwater samples in the Nile Delta region, Egypt. Acta Tropica 100 (1-2), 63-69.  (accession #’s DQ992178 – DQ992193).

Lorenzo-Morales, J., Martinez-Carretero, E., Batista, N., Alvarez-Marin, J., Bahaya, Y., Walochnik, J. and Valladares, B. 2007. Early diagnosis of amoebic keratitis due to a mixed infection with Acanthamoeba and Hartmannella. Parasitology Research 102: 167-169. (accession # EF205325).

Lorenzo-Morales J, Morcillo-Laiz R, Martín-Navarro CM, López-Vélez R, López-Arencibia A, Arnalich-Montiel F, Maciver SK, Valladares B, Martínez-Carretero E. 2011. Acanthamoeba keratitis due to genotype T11 in a rigid gas permeable contact lens wearer in Spain.  Contact Lens & Anterior Eye. 34: 83-6.  (accession #  HM778019).  

Lorenzo-Morales, J., Valladares, M., Sancho, J., Reyes-Batlle, M., Martin-Navarro, C.M., Lopez-Arencibia, A., González, A.C., Lopez-Medina, L., Pinero, J.E., Martinez-Carretero, E. and Valladares, B.  2013.  First Report of a Case of Prostatitis Due to Acanthamoeba in a Dog. Acta Protozool. (2013) 52: 325–329. (accession # JN555599).

Lorenzo-Morales,J., Reyes-Batlle,M., Arnalich-Montiel,F., Martin-Navarro,C.M., Valladares,B., Martinez-Carretero,E. and Pinero,J.E. 2014. Intraocular dissemination of Acanthamoeba keratitis after performing a tectonic penetrating keratoplasty: Serial cultures and PCR of aqueous humour to guide and monitor a successful treatment. Case report and review of literature.  Unpublished. (accession # JQ354982).

Lucena-Nunes, P., Gomez-Hernandez, C., Cembranelli, S.B.S., Nascentes, G.A.N. and Lages-Silva, E. 2018. Genetic characterization of Acanthamoeba spp and its relation with pathogens causing nosocomial infection in the UFTM clinic hospital, Uberaba-MG. Unpublished; Parasitology, Triangulo Mineiro Federal University, Uberaba, Minas Gerais, Brazil.  (accession # MF537185 – MF537200).

Maciver, S.K., Asif, M., Simmen, M.W. and Lorenzo-Morales, J.  2013. A systematic analysis of Acanthamoeba genotype frequency correlated with source and pathogenicity: T4 is confirmed as a pathogen-rich genotype. Eur. J. Protistol. 49: 217-221. (Accession # JX486704, JX494762 – JX494783).

Magnet,A., Henriques-Gil,N., Galvan,A.L., Izquierdo,F., Fenoy,S. and del Aguila,C.  2014. Novel Acanthamoeba 18S RNA Gene Sequence Type from an Environmental Isolate.  Parasitol. Res. 113: 2845–2850.   (accession # KJ413084).

Mafra,C.S., Carrijo-Carvalho,L.C., Chudzinski-Tavassi,A.M., Taguchi,F.M., Foronda,A.S., Carvalho,F.R. and de Freitas,D. 2013.  Antimicrobial action of biguanides on the viability of acanthamoeba cysts and assessment of cell toxicity. Invest. Ophthalmol. Vis. Sci. 54 (9), 6363-6372. (accession # KF318460 – KF318462).

Maghsood AH, Sissons J, Rezaian M, Nolder D, Warhurst D, Khan NA.  2005. Acanthamoeba genotype T4 from the UK and Iran and isolation of the T2 genotype from clinical isolates. J Med Microbiol. 54: 755-759.

Maghsoodloorad, S. 2015. Isolation of Acanthamoeba species from water in recreational areas of Golestan, north of Iran. Unpublished. (accession #’s KT215188 – KT215191).

Magliano,A.C., da Silva,F.M., Teixeira,M.M. and Alfieri,S.C. 2009. Genotyping, physiological features and proteolytic activities of a potentially pathogenic Acanthamoeba sp. isolated from tap water in Brazil. Experimental Parasitology 123: 231-235.  (Accession # FJ710616).   

Magliano,A.C., Teixeira,M.M. and Alfieri,S.C. 2012. Revisiting the Acanthamoeba species that form star-shaped cysts (genotypes T7, T8, T9, and T17): characterization of seven new Brazilian environmental isolates and phylogenetic inferences. Parasitology 139: 45-52.  (accession # JF325883 – JF325889).

Mahdavi Poor, B., Dalimi, A., Ghafarifar, F., Khoshzaban, F. and Abdolalizadeh, J. 2017. Genotyping of Acanthamoeba species isolated from keratitis patients by PCR method. Unpublished; Medical Parasitology, Tarbiat Modares University, Tehran, Iran. (Accession # MF576061 – MF576064)

Mahdavi Poor, B., Dalimi, A., Ghafarifar, F., Khoshzaban, F. and Abdolalizadeh, J.  2018. Contamination of swimming pools and hot tubs biofilms with Acanthamoeba. Acta Parasitologica, 2018, 63(1), 147–153. (accession # MF139785 – MF139795).

Mahmoudi, M.R., Rahmati, B., Seyedpour, S.H., and Karanis,P.  Occurrence and molecular characterization of free-living amoeba species (Acanthamoeba, Hartmannella, and Saccamoeba limax) in various surface water resources of Iran.  2015. Parasitol Res 114:4669 – 4674.  (accession # KR494228 – KR494242)

Makashova, M.A., Oglodin,E.G., Kukleva,L.M., Sharapova,N.A., Naryshkina,E.A., Germanchuk,V.G., Eroshenko,G.A. and Kutyrev,V. Long-Term Persistence of Yersinia pestis in Association with Acanthamoeba castellanii in Experiment. Problemy Osobo Opasnykh Infektsii [Problems of Particularly Dangerous Infections] – (4), 82-89 (2022)  (Acc # OR564015, OR564018 – OR564021).

Malavin, S. and Shmakova, L. 2018. New ancient Acanthamoeba from Siberian permafrost. Unpublished; Soil Cryology Laboratory, Institute of Physicochemical and Biological Problems in Soil Science, Pushchino, Moscow Region, Russia. (accession # MK124583 – MK124588).

Marciano-Cabral, F., Booton, G.C., Kelly, D.J., Ferguson, T., Powell, E. and Fuerst, P.A. 2005.  Burkholderia cepacia association with Acanthamoeba isolated from an AIDS patient with cutaneous acanthamoebiasis.  (Unpublished).  (accession #’s  DQ239700).   marciano-cabral-draft

Marinelli,K., Bagnarelli,P., Ferreri,M.L. and Trotta,D.  2012. A case of fatal disseminated Acanthamoeba infection in an immunocompromised patient not positive for HIV. Unpublished. (accession # JQ031557).

Maritschnegg, P., Sovinz, P., Lackner, H., Benesch, M., Nebl, A., Schwinger, W., Walochnik, J. and Urban, C. 2011. Granulomatous Amebic Encephalitis in a Child with Acute Lymphoblastic Leukemia Successfully Treated with Multimodal Antimicrobial Therapy and Hyperbaric Oxygen. J. Clin. Microbiol. 49 (1): 446-448. (accession # HQ450394).  

Marouf, M.A., Ashour, A.A., Soliman, M.I., Al-Herrawy, A.Z. and Gad, M.A., 2021. Acanthamoeba genotypes and Vermamoeba vermiformis in drinking water treatment facilities. Egyptian Journal of Aquatic Biology and Fisheries, 25(6), pp.327-341. (accession # OM003585OM003588, OM021886)

Martin-Navarro,C.M., Lorenzo-Morales,J., Cabrera-Serra,M.G., Rancel,F., Coronado-Alvarez,N.M., Pinero,J.E. and Valladares,B. 2008. The potential pathogenicity of chlorhexidine-sensitive Acanthamoeba strains isolated from contact lens cases from asymptomatic individuals in Tenerife, Canary Islands, Spain. Journal of Medical Microbiology 57: 1399-1404. (accession  #  EU686695 – EU686722) 

Martin-Perez, T., Criado-Fornelio, A., Martinez, J., Blanco, M.A., Fuentes, I. and Perez-Serrano, J. 2017. Isolation and molecular characterization of Acanthamoeba from patients with keratitis in Spain. Eur. J. Protistol. (2017) In press. (accession  # KY072778 – KY072783).

Martin-Perez, T., Criado-Fornelio, A., Avila-Blanco, M. and Perez-Serrano, J. 2017. New advances in the biology of Acanthamoeba spp. (Protozoa: Amoebozoa): an opportunistic pathogen found in contact lenses. (in) Arno,F. and Rein,E. (Eds.); Recent Progress in Eye Research: 1-90; Editorial Nova Science, NY, USA (2017) (accession # MF113385 , MF061315)

Maschio, V.J.,  Corcao, G., Bucker, F., Caumo, K., Rott, M.B. 2015.  Identification of Paenibacillus as a Symbiont in AcanthamoebaCurrent Microbiology 71: 415-420.  (Accession # KF801449 – KF801456). 

Maschio, V.J., Chies, F., Carlesso, A.M., Carvalho, A., Rosa, S.P., Van Der Sand, S.T., Rott, M.B.  2015.  Acanthamoeba T4, T5 and T11 Isolated From Mineral Water Bottles in Southern Brazil. Current Microbiology 70(1):6-9.  (accession # KJ531898 – KJ531909).

Maschio,V.J. and Rott,M.B.  2016. Isolation of Acanthamoeba from amoebic keratitis. Unpublished.  (accession #KX578215)

Mataji, S. and Khataminezhad, M.R.  2016. Isolation and identification of opportunistic amoeba from Tonekabon Rivers area. Unpublished .  (accession # KU364623).

Matthey-Doret, C., Colp, M.J., Escoll, P., Thierry, A., Curtis, B.A., Sarrasin, M., Gray, M.W., Lang, B.F., Archibald, J.M., Buchrieser, C. and Koszul, R. 2021.  bioRxiv preprint doi  : https://doi.org/10.1101/2021.10.26.465878  (genome accession # JAJGAO and JAJGAP)

Matsuo, J., Kawaguchi, K., Nakamura, S., Hayashi, Y., Yoshida, M., Takahashi, K., Mizutani, Y., Yao, T. and Yamaguchi, H. 2010.  Survival and transfer ability of phylogenetically diverse bacterial endosymbionts in environmental Acanthamoeba isolates. Environmental Microbiology Reports 2: 524-533. (Accession # AB506677 – AB506682; AB508961 – AB509001).    

Mbugua, M.W.  2008. Characterization of Unusual Gymnamoebae Isolated from the Marine Environment. M.S. thesis, Marshall University, Huntington, West Virginia, 126 pp. (seven undeposited sequences).

McKellar, M.S., Mehta, L.R., Greenlee, J.E., Hale, D.C., Booton, G.C., Kelly, D.J., Fuerst, P.A., Sriram, R. and Visvesvara, G.S.  2006.  Fatal Granulomatous Acanthamoeba Encephalitis Mimicking a Stroke, Diagnosed by Correlation of Results of Sequential Magnetic Resonance Imaging, Biopsy, In Vitro Culture, Immunofluorescence Analysis, and Molecular Analysis.  Journal of Clinical Microbiology 44: 4265-4269.  (accession #DQ339096).

Megha, K., Sehgal, R and Khurana, S. 2018. Genotyping of Acanthamoeba spp. isolated from patients with granulomatous amoebic encephalitis. Indian J Med Res 148: 456-459.  (accession # KX675337 – KX675341)

Megha, K., Sharma, M., Gupta, A., Sehgal, R., Khurana, S. 2018. Protein profiling of Acanthamoeba species using MALDI-TOF MS for specific identification of Acanthamoeba genotype. Parasitology Research (2018) 117:729–736. (accession # KU759839 – KU759840; KU175890 – KU175891; KX675337 – KX675341; KX709485 – KX709495; KY884984; KY859166 – KY859175).

Memari, F., M. Niyyati, A. Haghighi, S.J. Seyyed Tabaei and Z. Lasjerdi 2015. Occurrence of pathogenic Acanthamoeba genotypes in nasal swabs of cancer patients in Iran. Parasitol Res. 114: 1907-1912.  (accession #’s KJ786503 – KJ786526).

Memari, F., Niyyati, M., Lorenzo-Morales, J. and Jonaydi, Z.  2016.   Isolation and molecular characterization of Acanthamoeba strains isolated from the oral cavity of immunosuppressed individuals in Tehran, Iran. Acta Parasitol. 61 (3), 451-455.  (accession # KT894160 – KT894166)

Menacho, C., Soler, M., Chueca, P., Ormad, M.P. and Goñi, P., 2024. Chlorine Photolysis: A Step Forward in Inactivating Acanthamoeba and Their Endosymbiont Bacteria. Water, 16(5), p.668. (accession # OQ927217).

Mendoza, J.C.E., Enriquez, G.L. and Uemura, H.  2001.  Sequence analysis of the 18S rDNA of Philippine Acanthamoeba isolates (Unpublished).  (accession #’s AF443835 – AF443844).

Milanez, G., Masangkay, F., Hapan, F., Bencito, T., Ascano, A., Lizarondo, L., Lopez, M., Soriano, J., Santiago, J., Kotepui, M., Somsak, V., Tangpong, J. and Karanis, P. 2019. Identification of Acanthamoebae spp. using 18S rRNA primers in two major water reservoirs in the Philippines. Unpublished; Medical Technology, Far Eastern University Manila, Manila, Philippines. (acc #’s MK886460, MK886514, MK905437, MK909919, MK910997, MK9110210).

Milanez, G., Masangkay, F., Lirio, M.R., Hagosojos, B. and Karanis, P. 2019. Isolation of Acanthamoebae sp. in Selected Freshwater Fishes in the Philippines. Unpublished; Medical Technology, Far Eastern University Manila, Manila, Philippines. (acc #’s MN093961, MN093970 – MN093972).

Mohammadnajar, M., Ghaffarifar, F. and Dalimi, H. 2018. Isolation and Genotyping of Acanthamoeba Strains from soil sources from Varamin city, Iran. Unpublished; Medical Parasitology, Tarbiat Modares University, Tehran, Iran.  (acc # MF806034).

Mohd Hussain, R.H., Abd Ghani, M.K., Nordin, A. and Tengku Ahmad Basri, T.S.A. 2018. Vision Threatening Amoeba: Morphological And Molecular Based Evidence From Corneal Specimens Isolated In Malaysia. Unpublished; Centre of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam Campus, Kuala Selangor, Selangor, Malaysia.  (accession # MF576374 – MF576375).

Moreno-Mesonero, L., Moreno, Y., Alonso, J.L. and Ferrus, M.A. 2017. Detection of viable Helicobacter pylori inside free-living amoebae in wastewater and drinking water samples from Eastern Spain. Instituto de Ingenieria del Agua y Medio Ambiente, Universitat Politecnica de Valencia, Valencia, Spain. Unpublished. (Accession # MF399028 – MF399037).

Montalbano Di Filippo, M., Santoro, M., Lovreglio, P., Monno, R., Capolongo, C., Calia, C., Fumarola, L., D’Alfonso, R., Berrilli, F. and Di Cave, D. 2015.  Isolation and Molecular Characterization of Free-Living Amoebae from Different Water Sources in Italy. International Journal of Environmental Research and Public Health 12 (4), 3417-3427. (Accession # KP756942 – KP756959).

Motazedian,M.H. and Asgari,Q. 2015. Isolation and identification of pathogenic free living amoeba from surface and tap water of Shiraz city. Unpublished. (Accession #’s KP233865 – KP233872).

Mubareka, S., Alfa, M., Harding, G.K., Booton, G., Ekins, M. and Van Caeseele,.P. 2006. Acanthamoeba species Keratitis in a Soft Contact Lens Wearer Molecularly Linked to Well Water. Canadian Journal of Infectious Diseases and Medical Microbiology 17: 120–122. (accession # AY552093 – AY552096).

Murase, J. and Frenzel, P. 2008. Selective grazing of methanotrophs by protozoa in a rice field soil, FEMS Microbiology Ecology 65: 408-414. (accession #’s  AB425943 – AB425956)

Murase, J., Takenouchi, Y., Iwasaki, K. and Kimura, M.  2014.  Microeukaryotic Community and Oxygen Response in Rice Field Soil Revealed Using a Combined rRNA-Gene and rRNA-Based Approach. Microbes Environ. 29 (1), 74-81. (accession # AB769312).

Murase, J., Hida, A., Ogawa, K., Nonoyama, T., Yoshikawa, N. and Imai, K.  2014. Impact of long-term fertilizer treatment on microeukaryotic community structure in rice field soil. Unpublished. (accession # AB970156, AB970168, AB970210).

Nagyová, V.,  Nagy, A., Janeček,S. and Timko, J. 2010. Morphological, physiological, molecular and phylogenetic characterization of new environmental isolates of Acanthamoeba spp. from the region of Bratislava, Slovakia. Biologia 65: 81-91.  (accession #’s  GQ397463 – GQ397478).   

Nagyová, V., Nagy, A., Timko, J.  2010. Morphological, physiological and molecular biological characterisation of isolates from first cases of Acanthamoeba keratitis in Slovakia. Parasitology Research 106: 861-72.  (accession # GQ905495 – GQ905499  

Nayeri Chegeni, T. and Ghafari Far, F. 2016. Genotyping of Acanthamoeba species isolated from keratitis patients by PCR method. Unpublished. Medical Parasitology & Entomology Department, Tarbiat Modares University, Tehran, Iran.  (accession # KU877548 – KU877555).

Nazar, M., Haghighi, A., Niyyati, M., Eftekhar, M., Tahvildar-Biderouni, F., Taghipour, N., Abadi. A, Mojarad, E., and Athari, A. 2011. Genotyping of Acanthamoeba isolated from water in recreational areas of Tehran, Iran. Journal of Water and Health  9: 603-608. (Acccession #’s  AB525818 – AB525833).

Ng, S.L., Nordin, A., Abd Ghafar, N. and Chua, K.-H. 2017. Acanthamoeba-mediated cytopathic effect correlated with gene expression of MBP and AhLBP. Physiology Department, PPUKM, University Kebangsaan Malaysia. Unpublished. (Acccession #’s KY964312 – KY964321).

Ng, S.L., Nordin, A., Abd Ghafar, N. and Chua, K.-H. 2018.  Correlation between gene expression of MBP with cytopathic effect of environmental Acanthamoeba isolates in Malaysia.  Unpublished; Physiology Department, PPUKM, University Kebangsaan Malaysia, Malaysia.  (acc # MF197421 – MF197430).

Niyyati, M. and Karamati, S.A.  2016.  Isolation of Acanthamoeba genotypes from soil samples in Iran.  Unpublished.  (Accession # KT985962  – KT985978).

Niyyati, M., Lasjerdi, Z., Haghighi, A., Shahabi, S., Biderouni, F. T., Taghipour, N., Eftekhar, M., Mojarad, E. N. 2011. Contamination of hospital wards with pathogenic free living amoebae in Tehran, Iran.  Tropical Medicine & International Health 16 (Supplement: 1): 319-320. (accession #  JN036630 – JN036645).

Niyyati, M., Lasgerdi, Z. and Lorenzo-Morales, J.  2015.  Detection and Molecular Characterization of Potentially Pathogenic Free-living Amoebae from Water Sources in Kish Island, Southern Iran. Microbiol Insights 8 (SUPPL 1), 1-6.  (acc # KP337292 – KP337305).

Niyyati,M., Z. Lasjerdi, M. Nazar, A. Haghighi,and N. Mojarad. 2012.  Screening of recreational areas of rivers for potentially pathogenic free-living amoebae in the suburbs of Tehran, Iran. Journal of Water and Health  10: 140-146.  (accession # JF317322 – JF317334).

Niyyati,M., Lorenzo-Morales,J., Rahimi,F., Motevalli-Haghi,A., Martin-Navarro,C.M., Farnia,S., Valladares,B. and Rezaeian,M. 2009.  Isolation and genotyping of potentially pathogenic Acanthamoeba strains from dust sources in Iran. Transactions of the Royal Society of Tropical Medicine and Hygiene. 103: 425-427. (accession #’s FJ158856 – FJ158844).

Niyyati, M., Lorenzo-Morales, J., Rezaie,S., Rahimi, F., Mohebali,M., Maghsood, A.H., Motevalli-Haghi, A., Martin-Navarro, C.M., Farnia, S., Valladares, B. and Rezaeian, M. 2009. Genotyping of Acanthamoeba isolates from clinical and environmental specimens in Iran. Experimental Parasitology 121: 242-245. (accession #’s EU934046 – EU934072).

Niyyati M, Lorenzo-Morales J, Rezaie S, Rahimi F, Martín-Navarro CM, Mohebali M, Maghsood AH, Farnia S, Valladares B, Rezaeian M. 2010. First report of a mixed infection due to Acanthamoeba genotype T3 and Vahlkampfia in a cosmetic soft contact lens wearer in Iran. Experimental Parasitology 126: SI:89-90. (accession # EU683614 – EU683615).

Niyyati, M., Mafi, M. and Haghighi, A. and Hakemi Vala, M.  2015.  Occurrence of Potentially Pathogenic Bacterial-Endosymbionts in Acanthamoeba Spp.   Iranian Journal of Parasitology 10: 181-188. (accession # KJ504214 – KJ504227).

Niyyati, M., Memari, F., Haghighi, A. and Seyyed Tabaei, J.  2015.  Genotyping of Acanthamoeba strains isolated from mucosal tissue of  immunocompromised patients in Tehran, Iran. Unpublished.  (accession # KJ786502).

Niyyati, M., Nazar, M., Lasjerdi, Z., Haghighi, A. and Nazemalhosseini Mojarad,E.N.E. 2012. Acanthamoeba isolates belonging to T4 genotype in recreational water sources of Gilan Province, Northern Iran. (Unpublished).  Sequences related to:  Mahmoudi, MR, Taghipour, N., Eftekhar, M., Haghighi, A., Karanis, P. 2012.  Isolation of Acanthamoeba species in surface waters of Gilan province-north of Iran. Parasitology Research 110: 473-477.   (accession #’s JN399011 – JN399023).

Niyyati, M., Saberi, R., Latifi, A. and Lasjerdi, Z.  2016.  Distribution of Acanthamoeba Genotypes Isolated from Recreational and Therapeutic Geothermal Water Sources in Southwestern Iran.  Environ Health Insights 10, 69-74.   (Accession #’s KU356836 – KU356851).

Niyyati, M., Arab-Mazar, Z., Lasjerdi, Z., Lorenzo-Morales, J., Espotin, A., Yadegarynia, D., Gachkar, L. and Rahmati Roodsari, S. 2017. Molecular characterization of Acanthamoeba strains isolated from the oral cavity of hemodialysis patients in Iran. Parasitol. Res. (2017) In press.  (Accession #’s (MF176162 – MF176168).

Noinarin, P., Chareonsudjai, S. and Wangsomnuk, P. 2016. Direct Submission. Microbiology, Khon kaen University, Khon Kaen, Thailand.  (Accession # KX069000).

Norouznejadfard,M.J., Bahador,N., Solhjoo,K., Baserisalehi,M. and Armand,B.  2018. Isolation and characterization of free living amoeba from surface water of Fars province, Iran. Unpublished; Microbiology, IAU.Jahrom branch, Jahrom, Fars, Iran.  (Acc # KY981791 – KY981793).

Nuprasert, W., Putaporntip, C., Pariyakanok, L. and Jongwutiwes, S. 2010. Identification of a novel T17 genotype of Acanthamoeba from environmental isolates and T10 genotype causing keratitis in Thailand.  Journal of Clinical Microbiology 48: 4636-4640. (accession # GU808277 – GU8083290).  

Oglodin, E., Nikiforov, K., Guseva, N., Makashova, M., Eroshenko, G. and Kutyrev, V. 2017. Unpublished. Microbiology, Russian Research Anti-Plague institute ‘Microbe’, Universitetskaya, Saratov, Russia.  (accession # MG706258; MG708300)

Orosz, E., Farkas, A., Kodobocz, L., Becsagh, P., Danka, J., Kucsera, I. and Fuleky, G. 2013. Isolation of Acanthamoeba from the rhizosphere of maize and lucerne plants. Acta Microbiol Immunol Hung 60:(1)29-39. (accession # KC434439 – KC434451).

Otta, D.A., Rott, M.B., Carlesso, A.M. and da Silva, O.S.  2012. Prevalence of Acanthamoeba spp. (Sarcomastigophora: Acanthamoebidae) in wild populations of Aedes aegypti (Diptera: Culicidae).  Parasitology Research 111(5): 2017-2022.  (accession # JQ418494 – JQ41851). 

Otterholt, E. and Charnock, C. 2011. Identification and phylogeny of the small eukaryote population of raw and drinking waters. Water Res. 45 (8), 2527-253.  (Accession # JF317688).

Ovrutsky, A.R., Chan, E.D., Kartalija, M., Bai, X., Jackson, M. Gibbs, S., Falkinham, J.O. III, Iseman, M.D., Reynolds, P.R., McDonnell, G. and Thomas, V.  2013. Cooccurrence of Free-Living Amoebae and Nontuberculous Mycobacteria in Hospital Water Networks, and Preferential Growth of Mycobacterium avium in Acanthamoeba lenticulata. Appl. Environ. Microbiol. 79 (10), 3185-3192(Accession # KC669505 – KC669512). 

Ozkoc,S., Tuncay,S., Delibas,S.B., Akisu,C., Ozbek,Z., Durak,I. and Walochnik,J. 2008. Identification of Acanthamoeba genotype T4 and Paravahlkampfia sp. from two clinical samples. Journal of Medical Microbiology 57: 392-396.  (accession # EU266547 – EU266548).  

Padzik, M., Szostakowska, B., Szaflik, J. and Chomicz, L. 2017. Morpho-physiological and molecular diversity of selected pathogenic strains of Acanthamoeba identified in patients with AK in Poland 2009-2016. Unpublished. Department of Tropical Parasitology, Medical University of Gdansk, Poland.  (Accession # MF671962 – MF671968)

Pasricha, G., Das, D., Sharma, S., Booton, G.C. and Aggarwal, R.K.  2005. Understanding genetic structure of pathogenic isolates of Acanthamoeba spp. from non-contact lens related keratitis patients in India using 18S rDNA markers  (unpublished).  (accession #’s AY694138 – AY694145). 

Pasricha, G., Sharma, S., Garg, P. and Aggarwal, R.K.  2003.  Use of 18S rRNA gene-based PCR assay for diagnosis of acanthamoeba keratitis in non-contact lens wearers in India.  Journal of Clinical Microbiology 41: 3206-3211.   (accession #’s AF534135 – AF534156; AY690432AY690477).  

Patterson, D.J., Sogin, M.L. and Medlin, L. 1993.  unpublished.  (accession # L09599).

Pezeshki, A., Tajik, S. and Mahmoodzadeh, A. 2018. Parasitology, Zanjan University of medical Sciences, Karmandan, Zanjan 024, Iran.  (accession # MH620473 – MH620492).

Pezeshki, A., Kadkhodamohammadi, E., Mahmoodzadeh, A., Haniloo, A. 2017. The Acanthamoeba spp. in Water Sources from Zanjan Province, Northwest of Iran. Journal of Human, Environment and Health Promotion 2(3):168-176.   (accession # KX698425 -KX698433). 

Pezeshki, A., Tajic, S., Farahmandian, P., Haniloo, A., Mahmmodzadeh, A., Niyyati, M. and Behniafar, H., 2024. Phylogenetic analysis of Acanthamoeba isolated from soil samples and nasal cavities of patients with malignancy: a public health concern in the northwest of Iran. Transactions of The Royal Society of Tropical Medicine and Hygiene, p.trad100. (accession # MH539779 – MH539783 ; MH620473 – MH620492).

Petry,F., Torzewski,M., Bohl,J., Wilhelm-Schwenkmezger,T., Scheid,P., Walochnik,J., Michel,R., Zoller,L., Werhahn,K.J., Bhakdi,S. and Lackner,K.J.  2006.   Early Diagnosis of Acanthamoeba Infection during Routine Cytological Examination of Cerebrospinal Fluid.  Journal of Clinical Microbiology 44: 1903-1904.  (accession # DQ103890). 

Possamai, C.O., Loss, A.C., Costa, A.O., Falqueto, A. and Furst, C. 2018. Acanthamoeba of three morphological groups and distinct genotypes exhibit variable and weakly inter-related physiological properties. Parasitology Research 117:1389–1400. (acc #  MG825451 – MG825489).

Pourabbasi Ardekan, A., Niyyati, M., Saberi, R., Zanjirani Farahani, L. and Fatemi, M., 2023. Isolation and genotyping of Acanthamoeba species and Vahlkampfiidae in the harsh environmental conditions in the centre of Iran. Journal of Water and Health, 21(10), pp.1572-1579. (acc # OQ159094 – OQ159107)

Prithiviraj, S.R., Rajapandian, S.G.K., Gnanam, H., Gunasekaran, R., Mariappan, P., Singh, S.S., and Prajna, L. 2019. Clinical presentations, genotypic diversity and phylogenetic analysis of Acanthamoeba species causing keratitis. Journal of Medical Microbiology DOI 10.1099/jmm.0.001121  (acc# MK713899 – MK713927; MK578678)

Qvarnstrom Y., Nerad T.A., Visvesvara G.S. 2013. Characterization of a new pathogenic Acanthamoeba species, A. byersi n. sp., Isolated from a human with fatal amoebic encephalitis. J Eukaryot Microbiol 60: 626–633.  (accession # KC822461 – KC822470). 

Rahdar, M., Niyyati, M., Salehi, M., Feghhi, M., Makvandi, M., Pourmehdi, M. and Farnia, S. 2012. Isolation and Genotyping of Acanthamoeba Strains from Environmental Sources in Ahvaz City, Khuzestan Province, Southern Iran. Iranian Journal of Parasitology 8:186-189. (accession # JQ945976 – JQ945991).

Rahman, M.M., Yagita, K., Kobayashi, A., Oikawa, Y., Hussein, A.I., Matsumura, T. and Tokoro, M. 2013. Genetic Characterization of Clinical Acanthamoeba Isolates from Japan using Nuclear and Mitochondrial Small Subunit Ribosomal RNA. Korean Journal of Parasitology 51 (4), 401-411(accession # AB741044 – AB741047; AB795705 – AB795718). 

Ramírez-Flores, E., Bonilla-Lemus, P., Carrasco-Yépez, M.M., Ramírez-Flores, M.A., Barrón-Graciano, K.A., Rojas-Hernández, S., Reyes-Batlle, M. and Lorenzo-Morales, J., 2023. Saline-Tolerant Pathogenic Acanthamoeba spp. Isolated from a Geothermal Power Plant. Pathogens, 12(11), p.1363. (accession OR767826 – OR767831)

Rasti, S., Golestani, M.H., Hooshyar, H., Delavari, M. and Aghajani, A.  2016. Acanthamoeba sp. 18S ribosomal RNA genotypes from Iran. Parasitology, Kashan University of Medical Sciences, Kashan, Isfahan, Iran. Unpublished.  (accession #s LC177106 – LC177109; LC177665 – LC177667; LC179877 – LC179880).

Rasti, S., Golestani, M.H., Hooshyar, H., Delavari, M. and Aghajani, A.  2016. Acanthamoeba sp. 18S ribosomal RNA genotypes from Iran. Parasitology, Kashan University of Medical Sciences, Kashan, Isfahan, Iran. Unpublished. 

Rasti, S., Delavari, M., Golestani, M.H. and Hooshyar, H. 2017. Acanthamoeba genotypes for 18S ribosomal RNA. Parasitology, Kashan University of Medical Sciences, Kashan, Isfahan, Iran. Unpublished. (accession #s LC310895 – LC310897).

Rasti, S., Sabbaghian, M., Mostafaii, G.R., Iranshahi, L. and Mousavi, G.A. 2018. Acanthamoeba genotype T4 genes from springs, wells and underground water sources in Iran: Isfahan, Kashan. Unpublished. Kashan University of Medical Sciences, Parasitology; Kashan, Isfahan, Iran. (accession #s LC373012-LC373017).

Rayamajhee, B.; Subedi, D.; Won, S.; Kim, J.; Vijay, A.; Tan, J.; Henriquez, F.L.; Willcox, M.; Carnt, N.A. Investigating Domestic Shower Settings as a Risk Factor for Acanthamoeba Keratitis. Water 2020, 12, 3493. (sequences provided in suppl materials). 

Rayamajhee, B., Sharma, S., Willcox, M., Henriquez, F.L., Rajagopal, R.N., Shrestha, G.S., Subedi, D., Bagga, B. and Carnt, N., 2022. Assessment of genotypes, endosymbionts and clinical characteristics of Acanthamoeba recovered from ocular infection. BMC infectious diseases, 22(1), p.757. (acc # OK042094OK042106).

Rayamajhee, B., Williams, N.L., Siboni, N., Rodgers, K., Willcox, M., Henriquez, F.L., Seymour, J.R., Potts, J., Johnson, C., Scanes, P. and Carnt, N., 2023. Identification and quantification of Acanthamoeba spp. within seawater at four coastal lagoons on the east coast of Australia. Science of the Total Environment, 901, p.165862. (acc # OQ158980OQ158990)

Rayamajhee, B., Willcox, M., Sharma, S., Mooney, R., Petsoglou, C., Badenoch, P.R., Sherchan, S., Henriquez, F.L. and Carnt, N., 2024. Zooming in on the intracellular microbiome composition of bacterivorous Acanthamoeba isolates. ISME Communications, p.ycae016. (acc # OQ940657OQ940665)

Retana-Moreira, L., Abrahams-Sandi, E., Castro-Artavia, E., Fernandez-Sanchez, A., Castro-Castillo, A., Reyes-Batlle, M. and Lorenzo-Morales, J. 2015.  Isolation and Molecular Characterization of Acanthamoeba Strains from Dental Units in Costa Rica.  Journal of Eukaryotic Microbiology 2015 May 10. doi: 10.1111/jeu.12229. [Epub ahead of print].  (accession # KP677453 – KP677466).

Reyes-Batlle, M. 2019. Direct Submission; University Institu of Tropical Disease and Public Health of the Canary Islands, University of La Laguna, Santa Cruz de Tenerife, Spain.  (acc #’s MH842320 – MH842325).

Reyes-Batlle, M., Todd, C. D., Martín-Navarro, C. M., López-Arencibia,  A., Martín Cabello-Vilchez A., González, A.C., Córdoba-Lanús, E., Lindo, J. F.,  Valladares, B., Piñero,  J.E.,  Lorenzo-Morales, J. 2014. Isolation and characterization of Acanthamoeba strains from soil samples in Gran Canaria, Canary Islands, Spain.  Parasitology Research 113: 1383-1388. (accession # KF886742 – KF886765). 

Reyes-Batlle, M., Zamora-Herrera, J., Vargas-Mesa, A., Valeron-Tejera, M.A., Wagner, C., Martin-Navarro, C.M., Lopez-Arencibia,A., Sifaoui, I., Martinez-Carretero, E., Valladares, B., Pinero, J.E. and Lorenzo-Morales, J.  2016.  Acanthamoeba genotypes T2, T4, and T11 in soil sources from El Hierro island, Canary Islands, Spain. Parasitology Research 115(8):2953-6. (accession # KU885362- KU885380).

Richard, R.L., Ithoi, I., Majid, M.A.A., Sulaiman, W.Y.W., Tan, T.C., Nissapatorn, V. and Lim,Y. A.L.  2016.  Monitoring of Waterborne Parasites in Two Drinking Water Treatment Plants: A Study in Sarawak, Malaysia.  Int J Environ Res Public Health 13 (7), 641. (accession # KT356260 – KT356266).

Risler,A., Coupat-Goutaland,B. and Pelandakis,M. 2013. Genotyping and phylogenetic analysis of Acanthamoeba isolates associated with keratitis. Parasitology Research 112:  3807–3816.  (accession # HF930492 – HF930511). 

Rivera, W.L. and Adao, D.E. 2008. Identification of the 18S-ribosomal-DNA genotypes of Acanthamoeba isolates from the Philippines. Annals of Tropical Medicine and Parasitology 102: 671-677. (accession #’s  EU338502 – EU338511).     

Rivera, W.L. and Adao, D.E. 2009. 18S ribosomal DNA genotypes of Acanthamoeba species isolated from contact lens cases in the Philippines. Parasitology Research 105: 1119-1124.  (accesion #’s FJ815175  – FJ815190). 

Rocha-Cabrera, P., Reyes-Batlle, M., Martin-Navarro, C.M., Dorta-Gorrin, A., Lopez-Arencibia, A., Sifaoui, I., Martinez-Carretero, E., Pinero, J.E., Martin-Barrera, F., Valladares, B. and Lorenzo-Morales, J.  2015.  Detection of Acanthamoeba on the ocular surface in a Spanish population using the Schirmer strip test: pathogenic potential, molecular classification and evaluation of the sensitivity to chlorhexidine and voriconazole of the isolated Acanthamoeba strains. J. Med. Microbiol. 64 (8), 849-853. (accession #’s KP863871 – KP863887).

Rodriguez-Anaya, L.Z., Lares-Jimenez, L.F. and Lares-Villa, F. 2019. Direct Submission. Departamento de Ciencias Agronomicas y Veterinarias, Instituto Tecnologico de Sonora, Ciudad Obregon, Sonora, Mexico. (accession # MN096650)

Rojas, M.D.C., Rodriguez Fermepin, M., Gracia Martinez, F. and Costamagna, S.R. 2017. [Presence of Acanthamoeba spp. in watering trough in La Pampa province, Argentina]. Rev. Argent. Microbiol. 49(3):227-234. (accession #’s KX714006 – KX714012).

Rozo-Montoya, N., Bedoya-Urrego, K. and Alzate, J.F., 2023. Monitoring potentially pathogenic protists in sewage sludge using Metataxonomics. Food and Waterborne Parasitology, 33, p.e00210. (sequences not deposited, but available in supplemental materials).

Saberi, R., Fakhar, M., Sedighi, O., Espahbodi, F., Latifi, A., Makhlough, A., Sharifpour, A., Hezarjaribi, H.Z. and Asfaram, S. 2019. First molecular and morphological evidences of Acanthamoeba species (genotype T3, T4 and T5) in hemodialysis units in Iran. Acta Parasitologica 64: 911–915. (accession # MN339659 – MN339664).

Saitoh,Y., Hirano,S. and Nagaoka,T. 2013. Diversity of indigenous eukaryotic flora dominated by fungal species in Neogene sedimentary rocks of Horonobe, Hokkaido, Japan. Unpublished. (accession # AB830773 – AB830776).

Saberi, R., Najafi, A., and Naserifar, R. 2018. Isolation and determine genotype of Acanthamoeba spp. from dust sources in Ilam Province, West Iran. Acta Microbiologica et Immunologica Hungarica (in press 2019).  (accession # MK192784 – MK192801). 

Saitoh,Y., Hirano,S. and Nagaoka,T.  2013. Diversity of indigenous eukaryotic flora dominated by fungal species in Neogene sedimentary rocks of Horonobe, Hokkaido, Japan. Unpublished. (accession #AB830774 – AB830776).

Salehi,M., Mohammadrezapur,T. and Azadbaygi,M. 2013. (Acanthamoeba in North Khorasan, Iran), unpublished.  (accession # KF038130 – KF038139; KC295245).

Salehi, M. 2018. Direct Submission. Department of Parasitology, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Razavi Khorasan, Iran.  (accession # KY587113-KY587118, KY617798-KY617803)

Solhjoo, K., Rezanezhad, H., Biglarnia, F. and Armand, B. 2018. Free living amoeba isolated from haemodialysis units. Unpublished. Parasitology and Mycology, Jahrom University of Medical Sciences, Motahari, Jahrom, Fars, Iran.  (accession # KY514419KY514425)

Sampo,A., Matsuo,J., Yamane,C., Yagita,K., Nakamura,S., Shouji,N., Hayashi,Y., Yamazaki,T., Yoshida,M., Kobayashi,M., Ishida,K. and Yamaguchi,H. 2014. High-temperature adapted primitive Protochlamydia found in Acanthamoeba isolated from a hot spring can grow in immortalized human epithelial HEp-2 cells. Environmental Microbiology 16 (2), 486-497.  (accession # AB813163 – AB813164). 

Sanguino Jorquera, D.G., Poma, H.R., Irazusta, V.P., Juarez, M.M. and Rajal, V.B. 2019. Direct Submission. Insituto de Investigaciones para la Industria Quimica (INIQUI), Universidad Nacional de Salta (UNSa). CONICET, Salta, Argentina. (accession # MN071400 – MN071414).

Sant’Ana, V.P., Foronda, A.S., Freitas, D., Carrijo-Carvalho, L.C. and Carvalho, F.R.S. 2017.  Sensitivity of Enzymatic Toxins from Corneal Isolate of Acanthamoeba Protozoan to Physicochemical Parameters. Current Microbiology 74: 1316–1323. (accession # KX385274).

Sarr, P.S., Sugiyama, A., Begoude, A.D.B., Yazaki, K., Araki, S. and Nawata, E. 2018. Phylogenetic Diversity of Arbuscular Mycorrhizal Fungi in Cameroonian Cassava (Manihot esculenta Crantz) Fields based on Sequence Analysis of the 18S rRNA Region. Unpublished; Crop, Livestock and Environment, Japan International Research Center for Agricultural Science (JIRCAS), Tsukuba, Ibaraki, Japan.  (acc # KY020839).

Sarvi, S., Dodangeh, S. and Kialashaki, E. 2018. Isolation of Acanthamoeba genotype T4 from hot springs in Mazandaran Province, North of Iran. Unpublished; Parasitology and Mycology, Faculty of Medicine, Sari, Iran.  (accession # MG890336, MG890338).

Scheid,P., Balczun,C. and Schaub,G.A. 2014.  Some secrets are revealed: parasitic keratitis amoebae as vectors of the scarcely described pandoraviruses to humans. Parasitol. Res. 113 (10), 3759-3764.  (accession # KP236736). 

Scheid, P.L., Gruen,A.-L. and Michel, R. 2014. Genotyping of Acanthamoeba isolates from clinical and environmental samples in Germany.  Unpublished. (accession # KJ476512 – KJ476529).

Schmitz-Esser, S., Toenshoff, E.R., Haider, S., Heinz, E., Hoenninger, V.M., Wagner, M. and Horn, M.. 2008. Diversity of bacterial endosymbionts of environmental acanthamoeba isolates. Applied Environmental Microbiology 74: 5822-5831.  (accession # AM408788 – AM408803).   

Schmitz-Esser, S., Penz, T., Spang, A., Horn, M. 2011.  A bacterial genome in transition – an exceptional enrichment of IS elements but lack of evidence for recent transposition in the symbiont Amoebophilus asiaticus. BMC Evolutionary Biology 11: 270.  (accession # HM159368 – HM159370).  

Schroeder-Diedrich, J.M., Fuerst, P.A. and Byers, T.J.  1998.  Group-I introns with unusual sequences occur at three sites in nuclear 18S rRNA genes of Acanthamoeba lenticulata. Current Genetics 34:  71-78.  (accession # U94730 – U94741). 

Schroeder,J.M., Booton,G.C., Hay,J., Niszl,I.A., Seal,D.V., Markus,M.B., Fuerst,P.A. and Byers,T.J.  2001.  Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of acanthamoebae from humans with keratitis and from sewage sludge.   Journal of Clinical Microbiology 39: 1903-1911.  (accession #’s AF343801-AF343836;  AF343559 – AF343564).   

Scott, J.J., Lewin, G.R., Suen, G., Tringe, S.G., Barry, K.W., Touchon, J.C. and Currie, C.R.  2014. Leaf-cutter ant refuse dumps are nutrient reservoirs harboring diverse microbial assemblages.  Unpublished.  (Accession # LN588310, LN588174, LN587664)

Sente, C., Erume, J., Naigaga, I., Mulindwa, J., Ochwo, S., Magambo, P.K., Namara, B.G., Kato, C.D., Sebyatika, G., Muwonge, K. and Ocaido, M.  2016.   Prevalence of pathogenic free-living amoeba and other protozoa in natural and communal piped tap water from Queen Elizabeth protected area, Uganda. Infectious Diseases of Poverty 5:68.  (Accession # KU894799 – KU894804, KU884884)

Sharifi, N., Botero Kleiven, S., Ohman, D., Barragan, A. and Winiecka-Krusnell, J. 2010.  Genotypic characterization of Acanthamoeba spp. causing ocular infections in Swedish patients: identification of the T15 genotype in a case of protracted keratitis.  Scandinavian Journal of Infectious Diseases  42(10): 781-786.  (accession # HM036174  – HM036186).  

Sharma,S., Pasricha,G., Das,D. and Aggarwal,R.K.  2004.  Acanthamoeba keratitis in non-contact lens wearers in India: DNA typing-based validation and a simple detection assay. Archives of Ophthalmology 122 (10), 1430-1434.  (accession #’s AF534157 – AF534179).

Sheng, WH, Hung, CC,  Huang, HH, Liang, SY, Cheng, YJ, Ji, DD, Chang, SC. 2009.   Case Report: First Case of Granulomatous Amebic Encephalitis Caused by Acanthamoeba castellanii in Taiwan. American Journal of Tropical Medicine and Hygiene 81: 277-279. (accession # FJ707372 – FJ707373).    

Shokri, A., Sarvi, S., Daryani, A. and Sharif, M. 2016. Isolation and genotyping of Acanthamoeba Spp. as a neglected parasitic infection in north of Iran. Korean J Parasitol Vol. 54: 447-453. (Accession # KU872061  – KU872076).

Shyrobokov, V., Poniatovskyi, V., Yuryshynets, V.I., Chobotar, A. and Salamatin, R. 2017. Free-living Amoebas as a Representatives of Bentonite Clay’s Prokaryotic-eukaryotic Consortium. Mikrobiologichnyi Zhurnal 2017; 79(3):106-114. (Ukrainian).  (Accession # MH620775 – MH620777).

Silva,L.C.F., Dornas,F.P., Arantes,T., Abrahao,W.A.P., Kroon,E.G., Costa,A.O. and Abrahao,J.S. 2014. Characterization of isolates of Acanthamoeba from a Savanna-like Biome, Brazil. Unpublished.  (Accession # KM403405 – KM403423)

Soares, S.S., Souza, T.K., Berte, F.K., Cantarelli,V.V. and Rott, M.B. 2017. Occurrence of Infected Free-Living Amoebae in Cooling Towers of Southern Brazil. Current Microbiology 74:1461–1468. (accession # KX246887 – KX246901)

Solgi, R., Niyyati, M., Haghighi, A., Taghipour, N., Seyed Tabaei, S.J., Eftekhar, M. and Nazemalhosseini Mojarad, E. 2012.  Thermotolerant Acanthamoeba spp. isolated from therapeutic hot springs in Northwestern Iran.  Journal of Water and Health 10: 650-656. (accession # JN585806 – JN585817). 

Solgi, R. 2016. Isolation of Acanthamoeba species from water in recreational areas of Gorgan -north of Iran. Unpublished. Parasitology and Mycology, School of Medicine, Shiraz University of Medical Science, Shiraz, Iran  (accession # KX870199 – KX870203).

Solhjoo, K., Rezanezhad, H., Biglarnia, F. and Armand, B. 2018. Free living amoeba isolated from haemodialysis units. Unpublished. Parasitology and Mycology, Jahrom University of Medical Sciences, Motahari, Jahrom, Fars, Iran.  (accession # KY514419-KY514420, KY514421-KY514425 , KY488310 – KY488312)

Spanakos, G., Tzanetou, K., Miltsakakis, D., Patsoula, E., Malamou-Lada, E. and Vakalis, N.C.  2006.  Genotyping of pathogenic Acanthamoebae isolated from clinical samples in Greece – report of a clinical isolate presenting T5 genotype. Parasitology International 55: 147-149.    (accession #’s AY944575 – AY944580). 

Sriram, R., M. Shoff,  G. Booton,  P. Fuerst and  G.S. Visvesvara. 2008. Survival of  Acanthamoeba cysts after desiccation for more than 20 years.  Journal of Clinical Microbiology 46:4045-4048. (accession #’s FJ196642 – FJ196657; FJ218458). 

Stothard, D.R., Schroeder-Diedrich, J.M., Awwad, M.H., Gast, R.J., Ledee, D.R., Rodriguez- Zaragoza, S., Dean, C.L., Fuerst, P.A. and Byers, T.J. 1998.  The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. Journal of Eukaryotic Microbiology 45:  45-54(accession #’s AF019050 – AF019071). 

Taghadosi, Z., Hatam, G., Agholi, M., Ashraf, M.J. and Owji, N. 2016. Amoebic keratitis in Iranian patients. Unpublished.  (accession # KX387859) .

Taghipour, T., Rasti, S., Saba, M., Delavari, M., Moosavi, G.A., Hooshyar, H. and Eslamirad, Z., 2022. Molecular detection and genotype identification of Acanthamoeba species from bronchoalveolar lavage of patients with pulmonary symptoms suspected of cancer. Journal of Parasitic Diseases, 46(4), pp.1028-1035. (acc# LC522141 – LC522144; LC581471 – LC581474; LC598382 – LC598384; LC598498; LC598792; LC604810 – LC604813 )

Taher, E.E., Meabed, E.M., Abdallah, I. and Abdel Wahed, W.Y.  2017. Genotyping of Acanthamoeba strains amplified from corneal samples, in Egypt. Parasitology, Fayoum University, Faculty of Medicine, Fayoum, Egypt . Unpublished. (accession # MF350344 – MF350347).

Takamatsu,K., Isori,D., Ohashi,T., Nakamura,N., Hamatake,S. and Yanagida,J. 2013. Acanthamoeba 18S ribosomal RNA gene, partial sequence. Unpublished.  (accession # AB859622).

Takenouchi, Y., Iwasaki, K. and Murase, J.  2016. Response of the protistan community of a rice field soil to different oxygen tensions.  FEMS Microbiology Ecology 92: Article: fiw104 .  (accession # LC122938, LC122945, LC122948, LC122957, LC122960, LC122961).

Talarmin, J.P., Yera, H., Chretien, F., Plantin, P., Sihoan, P. and Hutin, P. 2017. Disseminated necrotic cutaneous nodules in a lung transplant recipient. Unpublished. Parasitology-Mycology, University Paris Descartes, University hospitals Paris Centre, Cochin hospital, Paris, France. (accession # MG020109)

Tanveer,T., Hameed,A., Muazzam, A.G., Jung,S.Y., Gul,A. and Matin,A.  2013. Isolation and molecular characterization of potentially pathogenic Acanthamoeba genotypes from diverse water resources including household drinking water from Khyber Pakhtunkhwa, Pakistan. Parasitology Research 112: 2925-2932.  (accession #’s KC203588 – KC203592; KC493773 – KC493777). 

Tanveer,T., Hameed,A., Muazzam,A.G., Gul,A. and Matin,A. 2013. Isolation and genotyping of Acanthamoeba from soil samples in Lahore, Pakistan. Unpublished. (accession # KC778186 – KC778190).

Tanveer,T., Hameed,A., Muazzam,A.G., Gul,A. and Matin,A.  2013.
Molecular identification and characterization of Acanthamoeba species in natural resources of Pakistan. Unpublished. (accession # KF035055 – KF035057).

Talarmin, J.P., Yera, H., Chretien, F., Plantin, P., Sihoan, P. and Hutin, P. 2017. Disseminated necrotic cutaneous nodules in a lung transplant recipient. Unpublished. Parasitology-Mycology, University Paris Descartes, University hospitals Paris Centre, Cochin hospital, Paris, France.  (accession # MG020109)

Teachachongchintana, N., Tananuvat, N., Somboon, P. and Wannasan, A. 2018. Acanthamoeba keratitis: first case in maharaj nakorn chiang mai hospital, Thailand. Unpublished; Department of Parasitology, Faculty of Medicine, CMU, Chiang Mai, Thailand. (accession # MH038174, MH038175).

Thammaratana, T., Laummaunwai, P. and Boonmars, T.  2016.  Isolation and identification of Acanthamoeba species from natural water sources in the northeastern part of Thailand. Parasitology Research 115:1705-1709.  (accession # KT897265, KT897274).

Thomas, V., Herrera-Rimann, K., Blanc, D.S., and Greub, G.  2006. Biodiversity of Amoebae and Amoeba-Resisting Bacteria in a Hospital Water Network. Applied Environmental Microbiology 72: 2428–2438.   (accession #’s AY237735).

Thomas,V., Loret,J.F., Jousset,M. and Greub,G. 2008. Biodiversity of amoebae and amoebae-resisting bacteria in a drinking water treatment plant. Environmental Microbiology 10: 2728-45. (accession #’s EU273822 – EU273873).  

Tice AK, Shadwick LL, Fiore-Donno AM, Geisen S, Kang S, Schuler GA, Spiegel FW, Wilkinson KA, Bonkowski M, Dumack K, Lahr DJG, Voelcker E, Clauß S, Zhang J & Brown MW. 2016. Expansion of the molecular and morphological diversity of Acanthamoebidae (Centramoebida, Amoebozoa) and identification of a novel life cycle type within the group. Biology Direct (2016) 11:69.  (accession # KX840327).

Todd,C.D., Reyes-Batlle,M., Martin-Navarro,C.M., Dorta-Gorrin,A., Lopez-Arencibia,A., Martinez-Carretero,E., Pinero,J.E., Valladares,B., Lindo,J.F. and Lorenzo-Morales,J. 2015.   Isolation and Genotyping of Acanthamoeba Strains from Soil Sources from Jamaica, West Indies. Journal of Eukaryotic Microbiology  62: 416-421.  (accession #  KM189369 – KM189437).

Trzyna, W.C., Mbugua, M.W., and Rogerson, A. 2010. Acanthamoeba in the Domestic Water Supply of Huntington, West Virginia, USA. Acta Protozoologica  49: 9-15. (accession #’s GU586221 – GU586224).  

Tsvetkova, N., Schild, M., Panaiotov, S.,  Kurdova-Mintcheva, R., Gottstein, B., Walochnik, J., Aspock, H., Siles, M. and Muller, N.  2004.  The identification of free-living environmental isolates of amoebae from Bulgaria. Parasitology Research 92: 405-13.  (accession #’s AY376159 – AY376163).  

Tuska-Szalay, B., Kelly, H., Takács, N., Kontschán, J., Votýpka, J. and Hornok, S., 2022. Molecular evidence of Monocercomonas and Acanthamoeba in the feces of captive reptiles. Parasitology Research, 121(12), pp.3681-3687. (accession OM455398 – OM455403).

Unger,C. and Lueck,C. 2012. Isolated Amoeba strain caused an eye infection. Unpublished. (accession # HE616185 – HE616186).

Uzuka,A., Hirooka,S., Knesaki,Y., Yoshikawa,H., Fujiwara,T. and Miyagishima,S. 2018. Responses of unicellular predators to cope with the phototoxicity of photosynthetic prey. Unpublished; National Institute of Genetics, Department of Symbiosis and Cell Evolution, Mishima, Shizuoka, Japan. (SRA # DRX121159).

Valladares, M., Reyes-Batlle, M., Mora-Peces, I., Martin-Navarro, C.M., Lopez-Arencibia, A., Dorta-Gorrin, A., Comyn-Afonso, E., Martinez-Carretero, E., Maciver, S.K., Pinero, J.E., Valladares, B. and Lorenzo-Morales, J. 2014. A multisystemic Acanthamoeba infection in a dog in Tenerife, Canary Islands, Spain. Veterinary Parasitology 205: 707-711.  (accession # KJ439562-KJ439567).

Valladares,M., Reyes-Batlle,M., Martin-Navarro,C.M., Lopez-Arencibia,A., Dorta-Gorrin,A., Wagner,C., Martinez-Carretero,E., Pinero,J.E., Valladares,B. and Lorenzo-Morales,J. 2015. Molecular characterization of Acanthamoeba strains isolated from domestic dogs in Tenerife, Canary Islands, Spain.  Archives of Microbiology (2015) 197(5):639-43.  (Accession #’s KP728281 – KP728282)

Valster,R.M., Wullings,B.A., Bakker,G., Smidt,H. and van der Kooij,D. 2009. Free-living protozoa in two unchlorinated drinking water supplies, identified by phylogenic analysis of 18S rRNA gene sequences. Applied and Environmental Microbiology 75: 4736-4746.  (accession # EU860702).     

Valster,R.M., Wullings,B.A. and van der Kooij,D. 2011.  Effect of temperature on the communities of free-living protozoa in four freshwater types in a biofilm batch system. Unpublished.  (accession # JF774851; JF775294 – JF775336).

van Zyl, L.M., Andrew, N., Chehade, M., Sadlon, T.A. and Badenoch,P.R. 2013. Acanthamoeba lenticulata keratitis in a hard contact lens wearer. Clin. Experiment. Ophthalmol. 41: 810-812.  (accession # KC438381).

Visvesvara,G.S., Booton,G.C., Kelley,D.J., Fuerst,P., Sriram,R., Finkelstein,A. and Garner,M.M.  2007.  In vitro culture, serologic and molecular analysis of Acanthamoeba isolated from the liver of a keel-billed toucan (Ramphastos sulfuratus). Veterinary Parasitology 143:  74-78.  (accession #’s  DQ451160 – DQ451163).    

Visvesvara, G.S., M.E. Shoff, R. Sriram, G.C. Booton, P.A. Fuerst, C.S. Hanley and M.M. Garner. 2010. Isolation, Morphologic, Serologic and Molecular Identification of Acanthamoeba T4 Genotype from the Liver of a Temminck’s Tragopan (Tragopan temminckii). Veterinary Parasitology 170:197-200. (accession # GQ889265). 

Wagner, C., Reyes-Batlle, M., Hernan, A., Rojas, E., Perez, G., Lopez-Arencibia, A., Sifaoui, I., Martinez-Carretero, E., Pinero, J.E., Valladares, B. and Lorenzo-Morales, J.  2016. High occurrence of Acanthamoeba genotype T4 in soil sources from Bolivar State, Venezuela. Acta Parasitol. 61: 466-470.  (accession # KU065124 – KU065137)

Wagner, C., Reyes-Batlle, M. and Lorenzo-Morales, J. 2016. Genotyping of clinical strains of Acanthamoeba genus in Venezuela. Unpublished. Parasitology, University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Tenerife, Canary Islands, Spain.  (accession # KT934531 – KT934547; KT95503)

Walia, R., Montoya, J.G., Visvesvera, G.S., Booton, G.C. and Doyle, R.L.  2007.  A case of successful treatment of cutaneous Acanthamoeba infection in a lung transplant recipient.  Transpl Infect Dis 9 (1), 51-54. (accession # KM099394)

Walochnik, J., Hassl, A., Simon, K., Benyr, G. and Aspock, H.  1999.  Isolation and identification by partial sequencing of the 18S ribosomal gene of free-living amoebae from necrotic tissue of Basilliscus plumifrons (Sauria: Iguanidae). Parasitology Research 85: 601-603.  (accession # AF114438 ). 

Walochnik, J., Haller-Schober, E., Kolli, H., Picher, O., Obwaller, A. and Aspock, H.  2000.  Discrimination between clinically relevant and nonrelevant Acanthamoeba strains isolated from contact lens- wearing keratitis patients in Austria.  Journal of Clinical Microbiology 38: 3932-3936.  (accession #’s AF251937 – AF251939). 

Walochnik, J., Obwaller, A. and Aspock, H.   2000.  Correlations between Morphological, Molecular Biological, and Physiological Characteristics in Clinical and Nonclinical Isolates of Acanthamoeba spp.   Applied Environmental Microbiology 66: 4408-4413.  (accession #’s AF260718 – AF260725).

Walochnik, J., Sommer, K., Obwaller, A., Haller-Schober, E.M., Aspock, H. 2004.  Characterisation and differentiation of pathogenic and non-pathogenic Acanthamoeba strains by their protein and antigen profiles. Parasitology Research 92: 289-298. (accession # AF28527). 

Walochnik, J., Aichelburg, A., Assadian, O., Steuer, A., Visvesvara, G., Vetter, N. and Aspock, H. 2008. Granulomatous amoebic encephalitis caused by Acanthamoeba amoebae of genotype T2 in a human immunodeficiency virus-negative patient.  J. Clin. Microbiol. 46:  338-340.   (accession # EF428121).  

Walochnik,J.  2012. Sequence of PB30/40  ATCC PRA-287.  personal communications  (not deposited; genome sequence now available; sequence of 18s rRNA from genome (pb30-40 18s rRNA sequence)).

Wannasan,A., Uparanukraw,P., Songsangchun,A. and Morakote,N. 2013. Potentially pathogenic free-living amoebae in some flood-affected areas during 2011 Chiang Mai flood. Rev. Inst. Med. Trop. Sao Paulo 55(6):411-416. (accession # JX507295). 

Weber,F., Mylnikov,A.P., Jurgens,K. and Wylezich,C. 2017. Culturing Heterotrophic Protists from the Baltic Sea: Mostly the ‘Usual Suspects’ but a Few Novelties as Well. J. Eukaryot. Microbiol. 64 (2), 153-163.   (accession # KX431477 – KX431477)

Weitere, M., Bergfeld, T., Rice, S.A., Matz, C. and Kjelleberg, S.  2005. Grazing resistance of Pseudomonas aeruginosa biofilms depends on type of protective mechanism, developmental stage and protozoan feeding mode. Environmental Microbiology 7: 1593-1601.  (accession # DQ013363).

Wildschutte, H. and Lawrence, J.G.  2007.  Differential Salmonella survival against communities of intestinal amoebae. Microbiology 153: 1781-1789.  (Accession #’s EF378666 – EF378696). 

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Wopereis, D.B., De Macedo, J.P., Rott, M.B., De Oliveira, J.G., Bazzo, M.L. and Caumo, K.S. 2018. Acanthamoeba spp. in hospital environments: characterization of Acanthamoeba spp. and amoeba-resistant microorganisms (ARMs) from air-conditioning systems . Unpublished; Clinical Analysis, Federal University of Santa Catarina, Campus Reitor Joao David Ferreira Lima, Florianopolis, Brazil  (accession # MF076628 – MF076666 ).

Xuan, Y-H. , Yu H.S., Jeong, H.J., Seol, S-Y., Chung, D-I. and Kong, H-H.  2007.  Molecular characterization of bacterial endosymbionts of Acanthamoeba isolates from infected corneas of Korean patients. Korean Journal of Parasitology 45: 1–9. (accession # EF140625 – EF140639). 

Xuan, Y., Shen, Y., Ge, Y., Yan, G. and Zheng, S. 2017. Isolation and identification of Acanthamoeba strains from soil and tap water in Yanji, China. Environmental Health and Preventive Medicine 22 (1), 58.  (Accession # KY827389 – KY827392).

Yera, H., Zamfir, O., Bourcier, T., Ancelle, T., Batellier, L., Dupouy-Camet,J . and Chaumeil, C.  2007.  Comparison of PCR, microscopic examination and culture for the early diagnosis and characterization of Acanthamoeba isolates from ocular infections. European Journal of Clinical Microbiology and Infectious Disease 26: 221-224.   (accession #’s DQ087287 – DQ087328)   

Yera, H., Zamfir, O., Bourcier, T., Viscogliosi, E., Noel, C., Dupouy-Camet, J. and Chaumeil, C. 2008.  The genotypic characterisation of Acanthamoeba isolates from human ocular samples. British Journal of Ophthalmology 92: 1139-1141.  (accession # DQ087287 – DQ087328; EU146068 – EU146073).

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