Publications from the OSU Acanthamoeba lab

Following is a list of the publications on research concerning Free-Living Amoebae from the Byers-Fuerst-Booton Lab:

Fani, M., Fuerst, P.A., Mosayebi, M., Javadi, A., Harandi, M.F., Saraei, M., Badri, M. and Hajialilo, E., 2022. Molecular identification and phylogenetic analysis of free-living amoeba in the water resources of Arak, Iran. Journal of Water and Health. JWH2022030.

Fuerst, P.A., and Booton, G.C. 2020. Species, Sequence Types and Alleles: Dissecting Genetic Variation in Acanthamoeba. Pathogens 2020, 9, 534; doi:10.3390/pathogens9070534

Borquez-Román, M.A., Lares-Jiménez, L.F., Rodriguez-Anaya, L.Z., Gonzalez-Galaviz, J.R., Fuerst, P.A., Ibarra-Gámez, J.C., Casillas-Hernández, R., and Lares-Villa, F. 2020. Stenamoeba dejonckheerei sp. nov., a Free-Living Amoeba Isolated from a Thermal Spring. Pathogens 2020, 9(7), 586;

Fuerst, P.A., Booton, G.C., Crary, M. 2015. Phylogenetic Analysis and the Evolution of the 18S rRNA Gene Typing System of Acanthamoeba. Journal of Eukaryotic Microbiology 62: 69-84. doi: 10.1111/jeu.12186

Lares-Jiménez, L.F., G.C. Booton, F. Lares-Villa, C.A. Velázquez-Contreras, and P.A. Fuerst. 2014. Genetic analysis among environmental strains of Balamuthia mandrillaris recovered from an artificial lagoon and from soil in Sonora, Mexico. Experimental Parasitology 145: S57-S61; doi: 10.1016/j.exppara.2014.07.007

Fuerst, P.A.  2014. Insights from the DNA databases: Approaches to the phylogenetic structure of Acanthamoeba.  Experimental Parasitology 145: S39-S45; doi: 10.1016/j.exppara.2014.06.020. Fuerst – Experimental Parasitology.

Kent, M., S.R. Platt, R.R. Rech, J.S. Eagleson, E.W. Howerth, M. Shoff, P.A. Fuerst, G. Booton,  G.S. Visvesvara and S.J. Schatzberg. 2011. A multi-systemic infection with an Acanthamoeba species in a dog.  Journal of the American Veterinary Medical Association 238: 1476-1481.

Tu, E.Y., C.E. Joslin, and M.E. Shoff. 2010.  Successful Treatment of Chronic Stromal Acanthamoeba Keratitis With Oral Voriconazole Monotherapy. CORNEA   29: 1066-1068.

Tu, E.Y., C.E. Joslin, PhD, M.E. Shoff, J.A. Lee, P.A. Fuerst. 2010.  Sequential Corneal Infection with Two Genotypically Distinct Acanthamoebae Associated with Renewed Contact Lens Wear.  EYE 24: 1119-1121.

Joslin CE, Tu EY, Shoff ME, Anderson RJ, Davis FG. 2010 Shifting distribution of Chicago-area Acanthamoeba keratitis cases. Archives of Ophthalmology. 128: 137-9.

Visvesvara, G.S., M.E. Shoff, R. Sriram, G.C. Booton, P.A. Fuerst, C.S. Hanley and M.M. Garner. 2010. Isolation, Morphologic, Serologic and Molecular Identification of Acanthamoeba T4 Genotype from the Liver of a Temminck’s Tragopan (Tragopan temminckii). Veterinary Parasitology 170:197-200. Visvesvara – VetParasit 2010

Tu EY, Joslin CE, Nijm LM, Feder RS, Jain S, Shoff ME.  2009.  Polymicrobial keratitis: Acanthamoeba and infectious crystalline keratopathy. American Journal of Ophthalmology 148:13-9.

Booton, G.C., C.E. Joslin, M. Shoff, E.Y. Tu, D.J. Kelly, and P.A. Fuerst. 2009.  Genotypic Identification of Acanthamoeba sp. Isolates Associated with an Outbreak of Acanthamoeba keratitis. Cornea 28: 673-676.

Tu, E.Y.  C.E. Joslin, J. Sugar, M.E. Shoff, G.C. Booton. 2008. Prognostic Factors Affecting Visual Outcome in Acanthamoeba Keratitis. Ophthalmology 115: 1998-2003.

Sriram, R., M. Shoff,  G. Booton,  P. Fuerst and  G.S. Visvesvara. 2008. Survival of  Acanthamoeba cysts after desiccation for more than 20 years.  Journal of Clinical Microbiology 46:4045-4048.

Shoff ME, Rogerson A, Kessler K, Schatz S, Seal DV. 2008. Prevalence of Acanthamoeba and other naked amoebae in South Florida domestic water.  Journal of Water and Health 6: 99-104.

Tu E.Y., C.E. Joslin, J. Sugar, G.C. Booton, M.E. Shoff and P.A. Fuerst. 2008. The relative value of confocal microscopy and superficial corneal scrapings in the diagnosis of Acanthamoeba keratitis. Cornea 27: 764-772.

Shoff, M.E., C.E. Joslin, E.Y. Tu, L. Kubatko and P.A. Fuerst. 2008. Efficacy of Contact Lens Systems Against Recent Clinical and Tap Water Acanthamoeba Isolates. Cornea 27: 713-719.

Visvesvara, G.S., G.C. Booton, D.J. Kelley, P.A. Fuerst, R. Sriram, A. Finkelstein, M.M. Garner. 2007. In vitro culture, serologic and molecular analysis of Acanthamoeba isolated from the liver of a keel-billed toucan (Ramphastos sulfuratus). Veterinary Parasitology 143: 74–78.

Walia R, Montoya JG, Visvesvera GS, Booton GC, Doyle RL.. 2007. A case of successful treatment of cutaneous Acanthamoeba infection in a lung transplant recipient. Transplant Infectious Disease 9: 51-54.

Kinde, H., D.H. Read, B.M. Daft, M. Manzer, R.W. Nordhausen, D.J. Kelly, P.A. Fuerst, G. Booton and G.S. Visvesvara. 2007.  Infections Caused by Pathogenic Free-living Amebas (Balamuthia mandrillaris and Acanthamoeba Sp.) in Horses.  Journal of Veterinary Diagnostic Investigation 19: 317-322.

Joslin, C.E., E.Y. Tu,  M.E. Shoff, G.C. Booton, P.A. Fuerst, T.T. McMahon, R.J. Anderson, M.S. Dworkin, J. Sugar,  F.G. Davis and  L.T. Stayner. 2007. The association of contact lens solution use and Acanthamoeba keratitis. American Journal of Ophthalmology 144: 169-180

Fuerst, P.A. and G.C. Booton. 2007. An Update on the Acanthamoeba DNA Sequence Database at The Ohio State University: A Resource for the Study of Clinical and Environmental Isolates. Proceedings of the XIIth International Meeting on the Biology and Pathogenicity of Free-living Amoebae, Wako City, Japan, p. Po-01. Japanese Journal of Protozoology 40: 178.

Booton, G.C., C.E. Joslin, M. Shoff, E.Y. Tu, D.J. Kelly, and P.A. Fuerst. 2007.  Molecular Identification of Acanthamoeba sp. Isolates in an Acanthamoeba Keratitis Outbreak.  Proceedings of the XIIth International Meeting on the Biology and Pathogenicity of Free-living Amoebae, Wako City, Japan, p. Po-10. Japanese Journal of Protozoology 40: 182-183.

Shoff, M.E., C.E. Joslin, G.C. Booton, E.Y. Tu, and P.A. Fuerst. 2007. Prevalence of Acanthamoeba in Chicago Area Tap Water.  Proceedings of the XIIth International Meeting on the Biology and Pathogenicity of Free-living Amoebae, Wako City, Japan, p. Po-14. Japanese Journal of Protozoology 40: 184-185.

Mubareka S, Alfa M, Harding GK, Booton G, Ekins M, Vancaeseele P. 2006.  Acanthamoeba species Keratitis in a Soft Contact Lens Wearer Molecularly Linked to Well Water. Canadian Journal of Infectious Diseases and Medical Microbiology 17: 120–122

McKellar, M.S., L.R. Mehta, J.E. Greenlee, D.C. Hale, G.C. Booton, D.J. Kelly, P.A. Fuerst, R. Sriram and G.S. Visvesvara. 2006. Fatal granulomatous Acanthamoeba encephalitis mimicking a stroke, diagnosed by correlation of results of sequential magnetic resonance imaging, biopsy, in vitro culture, immunofluorescence analysis, and molecular analysis. Journal of Clinical Microbiology 44: 4265-4269.

Dubey JP, Benson JE, Blakeley KT, Booton GC, Visvesvara GS. 2005. Disseminated Acanthamoeba sp. infection in a dog.  Veterinary Parasitology 128: 183-187

Booton, G.C., G.S. Visvesvara, T.J. Byers, D.J. Kelly, and P.A. Fuerst. 2005. Identification and distribution of Acanthamoeba species genotypes associated with nonkeratitis infections..  Journal of Clinical Microbiology 43: 1689-1693.

Yagi S, Booton GC, Visvesvara GS, Schuster FL. 2005.  Detection of Balamuthia mitochondrial 16S rRNA gene DNA in clinical specimens by PCR.  Journal of  Clinical Microbiology 43: 3192-3197.

D.V. Seal, M. Shoff, A. Rogerson, S. Schatz, H. Park, T. Beattie, A. Tomlinson and G. Booton. 2005.  Varying Susceptibility of Corneal and Environmental Strains of Acanthamoebae to Multipurpose Solutions using a Novel Method. Meeting of the Association for Research in Vision and Ophthalmology (ARVO 2005). Investigative Ophthalmology & Visual Science 46:   Supplement: S, 916.   

Booton, G.C.,  A. Rogerson, T.D. Bonilla, D.V. Seal, D.J. Kelly, T.K. Beattie, A. Tomlinson, F. Lares-Villa, P.A. Fuerst, T.J. Byers.  2004.   Molecular and Physiological Evaluation of Subtropical Environmental Isolates of Acanthamoeba spp., Causal Agent of Acanthamoeba Keratitis.  Journal of Eukaryotic Microbiology 51: 192-200.

Dunnebacke TH, Schuster FL, Yagi S, Booton GC. 2004. Balamuthia mandrillaris from soil samples. Microbiology 150: 2837-2842.

Booton, G.C., Carmichael, J.R., Visvesvara, G.S., Byers, T.J., Fuerst, P.A. 2003.  Genotyping of Balamuthia mandrillaris based on nuclear 18S and mitochondrial 16S rRNA genes. American Journal of Tropical Medicine and Hygeine. 68(1): 65-69

Booton, G.C., F. Lares-Villa, D.J. Kelly, P.A. Fuerst and T.J. Byers. 2003. Multiple alleles of Acanthamoeba nuclear small subunit ribosomal RNA genes: further evidence of possible genetic exchange between closely related strains.  Xth International Meeting on the Biology and Pathogenicity of Free-Living Amoebae Proceedings, pp. 83-91, ITSON-DIEP, Cd. Obregon, Mexico.

Booton, G.C.,  F.L. Schuster, J.R. Carmichael, P.A. Fuerst, T.J. Byers. 2003. Balamuthia mandrillaris: Identification of Clinical and Environmental Isolates Using Genus-Specific PCR. Journal of Eukaryotic Microbiology 50: 508-509.

Dunnebacke TH, Schuster FL, Yagi S, Booton GC. 2003. Isolation of Balamuthia amebas from the environment. Journal of Eukaryotic Microbiology 50 Suppl: 510-511.

Fuerst, P.A.,  G.C. Booton, G.S. Visvesvara, T.J. Byers. 2003. Genotypic Identification of Strains of the Opportunistically Pathogenic Ameba Genus Acanthamoeba in Non- Keratitis Infections. Journal of Eukaryotic Microbiology 50: 512-513

Ledee,  D.R., G.C. Booton, M.H. Awwad, S. Sharma, R.K. Aggarwal, I.A. Niszl, M.B. Markus, P.A. Fuerst, and T.J. Byers.  2003.  Advantages of Using Mitochondrial 16S rDNA Sequences to Classify Clinical Isolates of AcanthamoebaInvestigative Ophthalmology and Visual Science 44: 1142-1149

Booton, G.C.,  A. Rogerson, T. Davidian, D. Seal, D. Kelly, T. Beattie, A. Tomlinson, F. Lares, P. Fuerst, T. Byers.  2003.   Molecular and Physiological Evaluation of Subtropical Environmental Isolates of Acanthamoeba spp., Causal Agent of Acanthamoeba Keratitis.  2003 Annual Meeting of the Association for Research in Vision and Ophthalmology, ARVO,  Proceedings:  Abstract 1416;  page 58.

Booton, G.C., J.R. Carmichael, G.S. Visvesvara, T.J. Byers and P.A. Fuerst. 2003.  Identification of Balamuthia mandrillaris by PCR assay using the mitochondrial 16S rRNA gene as a target.  Journal of Clinical Microbiology  41: 453-455.

Schuster FL, Dunnebacke TH, Booton GC, Yagi S, Kohlmeier CK, Glaser C, Vugia D, Bakardjiev A, Azimi P, Maddux-Gonzalez M, Martinez AJ, Visvesvara GS. 2003.  Environmental isolation of Balamuthia mandrillaris associated with a case of amebic encephalitis. Journal of Clinical Microbiology 41: 3175-3180.

Booton, G.C., D.J. Kelly, Y-W. Chu, D. Seal, E. Houang, D. Lam, T.J. Byers and P.A. Fuerst. 2002. 18S rDNA Sequence Typing and Tracking of Acanthamoeba sp. from Corneal Scrapes, Contact Lenses, Lens Cases and Home Water Supplies of Acanthamoeba Keratitis Patients in Hong Kong.   J. Clinical Microbiology 40: 1621-1625.

Booton, G.C., D.R. Ledee, M. Awwad, S. Sharma, I. Nizsl, M.M. Markus, P.A. Fuerst and  T. J. Byers. 2001. Acanthamoeba Mitochondrial 16S rDNA Sequences: Inferred Phylogeny and Support of Nuclear Ribosomal 18S rDNA Gene Sequence Types.  IXth International Meeting on the Biology and Pathogenicity of Free-Living Amoebae Proceedings: 219-226.

Byers, T.J.,  G.C. Booton, D.R. Stothard, R.J. Gast, D.R. Ledee, J.M. Schroeder, M. Awwad, and P.A. Fuerst. 2001. Studies of the Phylogeny, Systematics and Pathogenicity of Acanthamoeba Using Ribosomal and Transfer RNA Gene Sequences.   IXth International Meeting on the Biology and Pathogenicity of Free-Living Amoebae Proceedings: 211-218.

Schroeder, J.M.., G.C. Booton, J. Hay, I. Niszl, D.V. Seal, M.B. Markus, P.A. Fuerst and T.J. Byers. 2001.  Use of Subgenic 18S rDNA PCR and Sequencing for Genus and Genotype Identification of Acanthamoeba from Human with keratitis and from Sewage Sludge. J. Clinical Microbiology 39: 1903-1911.

Dyková I, Lom J, Schroeder-Diedrich JM, Booton GC, Byers TJ. 1999. Acanthamoeba strains isolated from organs of freshwater fishes. Journal of Parasitology 85: 1106-1113

Stothard, D.R., Hay, J., Schroeder-Diedrich, J.M., Seal, D.V., Byers, T.J..  1999.  Fluorescent oligonucleotide probes for clinical and environmental detection of Acanthamoeba and the T4 18S rRNA gene sequence type.  Journal of Clinical Microbiology 37: 2687-93

Ledee, D.R., Seal, D.V., Byers, T.J. 1998.  Confirmatory evidence from 18S rRNA gene analysis for in vivo development of propamidine resistance in a temporal series of Acanthamoeba ocular isolates from a patient. Antimicrobial Agents and Chemotherapy. 1998 42: 2144 – 2145

Schroeder-Dietrich, M.H., P.A. Fuerst and T.J. Byers. 1998. Group-I introns with unusual sequences occur at three sites in nuclear 18S rRNA genes of Acanthamoeba lenticulataCurrent Genetics 34: 71-78.

Stothard, D.S., J.M. Schroeder-Dietrich, M.H. Awwad, R.J. Gast, D.R. Ledee, S. Rodriguez-Zaragoza, C.L. Dean, P.A. Fuerst and T.J. Byers. 1998. The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. Journal of Eukaryotic Microbiology:  45: 45-54

Ledee, D.R., Hay, J., Byers, T.J., Seal, D.V., Kirkness, C.M. 1996. Acanthamoeba griffini. Molecular characterization of a new corneal pathogen.  Investigative Ophthalmology and Visual Sciences. 37: 544 – 550

Gast, R.J., D.R. Ledee, P.A. Fuerst and T.J. Byers. 1996.  Subgenus systematics of Acanthamoeba: Four nuclear 18S rRNA sequence types. Journal of Eukaryotic Microbiology: 43: 498-504.

Gast, R.J., Byers, T.J. 1995.  Genus-specific and Subgenus-specific Oligonucleotide Probes for Acanthamoeba. Molecular and Biochemical Parasitology 71: 255-260.

Weekers, P.H.H., R.J. Gast, P.A. Fuerst and T.J. Byers. 1994.  Sequence variations in small-subunit ribosomal RNAs of Hartmannella vermiformis and their phylogenetic implications. Molecular Biology and Evolution 11: 684-690.  

Gast, R.J., Fuerst, P.A., Byers, T.J.  1994.  Discovery of group I introns in the nuclear small subunit ribosomal RNA genes of Acanthamoeba. Nucleic Acids Research. 22(4):592-6

Hugo, E.R., Byers, T.J.  1993. S-adenosyl-L-methionine decarboxylase of Acanthamoeba castellanii (Neff): purification and properties.  The Biochemical Journal 295( Pt 1): 203-9

Byers, T.J., Kim, B.G., King, L.E., Hugo, E.R. 1991  Molecular aspects of the cell cycle and encystment of Acanthamoeba.  Reviews of Infectious Diseases 13: Suppl 5 S373-84

Byers, T.J., Hugo, E.R., Stewart, V.J. 1990.  Genes of Acanthamoeba: DNA, RNA and protein sequences (a review).  Journal of Protozoology. 37(4): 17S-25S

Kim, B.G., McCann, P.P., Byers, T.J.  1987.  Inhibition of multiplication in Acanthamoeba castellanii by specific inhibitors of ornithine decarboxylase.  Journal of Protozoology 34(3): 264-6.

Kim, B.G., Sobota, A., Bitonti, A.J., McCann, P.P., Byers, T.J. 1987.  Polyamine metabolism in Acanthamoeba: polyamine content and synthesis of ornithine, putrescine, and diaminopropane. Journal of Protozoology 34(3): 278-84

Byers, T.J.  1986. Molecular-biology of DNA in Acanthamoeba, Ameba, Entamoeba, and Naegleria. International Review of Cytology 99: 311-341.  

Akins, R.A., S.M. Gozs and T.J. Byers. 1985. Factors Regulating the Encystment Enhancing Activity (EEA) of Acanthamoeba castellanii. Journal of General Microbiology 131:   2609-2617.

Byers, T.J., L.L. Burianek, V. Stewart, and B.G. Kim. 1984. Mitochondrial DNA variation: A basis for the taxonomy of Acanthamoeba. Journal of Protozoology 31: (4) A18-A19. (Abstract).

Byers, T.J., S.A. Bogler and L.L. Burianek. 1983. Analysis of Mitochondrial-DNA Variation as an Approach to Systematic Relationships in the Genus Acanthamoeba. Journal of Protozoology  30: (2) 198-203.

Bogler, S.A., C.D. Zarley, L.L. Burianek, P.A. Fuerst, and T.J. Byers. 1983.  Interstrain Mitochondrial-DNA Polymorphism Detected in Acanthamoeba by Restriction Endonuclease Analysis.  Molecular and Biochemical Parasitology 8: (2) 145-163.

Seilhamer, J.J. and T.J. Byers. 1982. ATPase-associated Oligomycin Resistance in Acanthamoeba castellanii. Journal of Protozoology 29: (3) 394-397

Akins, R.A., S.M. Gozs and T.J. Byers. 1982.  Dependence of encystment on encystment enhancing activity in Acanthamoeba. Journal of Protozoology 29: (3) 473 (abstract).

Akins, R.A. and T.J. Byers. 1980.  Differentiation Promoting Factors Induced in Acanthamoeba by Inhibitors of Mitochondrial Macromolecule Synthesis. Developmental Biology  78: (1) 126-140.

Byers, T.J., Akins, R.A., Maynard, B.J.,  Lefken, R.A., Martin, S.M. 1980. Rapid Growth of Acanthamoeba in Defined Media – Induction of Encystment by Glucose-acetate Starvation. Journal of Protozoology 27: (2) 216-219.

Seilhamer, J.J. and T.J. Byers. 1978.  Mutants of Acanthamoeba castellanii Resistant to Erythromycin, Chloramphenicol, and Oligomycin. Journal of Protozoology  25: (4) 486-489.   

Martin, S.M. and Byers, T.J. 1977. Evidence for Lysosomal Enzymes in Acanthamoeba and Their Activity Changes During Encystment. Ohio Journal of Science  77: (1) 28-35.

Martin, S.M. and , Byers, T.J. 1976. Acid Hydrolase Activity During Growth and Encystment in Acanthamoeba castellanii.  Journal of Protozoology 23: (4) 608-613.

Mattar, F.E. and T.J. Byers. 1971. Morphological changes and the requirements for macromolecule synthesis during excystment of Acanthamoeba castellanii. Journal of Cell Biology 49(2):507-19.  

Byers, T.J., Rudick, V.L., Rudik, M.J. 1969.  Cell Size, Macromolecule Composition, Nuclear Number, Oxygen Consumption and Cyst Formation During Two Growth Phases in Unagitated Cultures of Acanthamoeba castellanii. Journal of Protozoology 16: (4) 693-699.

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