Fern Agar [BOLD’S MINERAL NUTRIENT MEDIUM]

distilled H2O………………………..1L
NaNO3 macronutrient…………..10mL
CaCl2 macronutrient……………..10mL
K2HPO4 macronutrient…………10mL
KH2PO4 macronutrient…………10mL
MgSO4.7H2O macronutrient….10mL
NaCl macronutrient……………….10mL
Micronutrient stock solution…..2mL
1%FeCl3………………………………..1 drop
bactoagar………………………………7g

See recipes for these items below.

Macronutrient Stock Solutions

NaNO3………………………10g/400mL H2O
CaCl2…………………………1g/400mL H2O
K2HPO4…………………….3g/400mL H2O
KH2PO4…………………….7g/400mL H2O
MgSO4.7H2O……………..3g/400mL H2O
NaCl…………………………..1g/400mL H2O

Micronutrient Stock Solution [Nitsch’s Micronutrients, Fe, and Co]

distilled H2O……………….1L
H2SO4 (conc)………………0.5mL
MnSO4.4H2O………………3g
ZnSO4.7H2O……………….500mg
H3BO3………………………..500mg
CuSO4.5H2O……………….25mg
CoCl2………………………….25mg
Na2MoO4.2H2O………….25mg

Iron Solution [1% FeCl3]