Port Parameters
- Each channel will have one entrance port and one exit port.
- The ports will be 4mm in diameter.
- The entrance port will be circular and will smoothly flow into the channel in order to minimize entrance length.
- The exit port will be circular and have a standard connection to the channel.
- The entrance port selected is better because it minimizes entrance length. However, a small part of constant sized channel must be sacrificed.
Channel Parameters
- The chip will have 4 channels.
- Each channel will be at depth 125 µm.
- Two of the channels will have length of 4 cm.
- Width: One channel at 300 µm, other at 400 µm.
- Two of the channels will have length of 3 cm.
- Width: One channel at 300 µm, other at 400 µm.
- The channels will be placed as far away from each other as possible to maximize space usage on the wafer.
- The specifications shown above allow for a variability in channel conditions but limit the efficiency of repeatability with any one type of channel.
Consideration of Testing Region
- The testing region will be found by first determining the entrance length based on the fluid in question. Any point after the entrance length up to the exit port will be viable channel length for usage as the testing region. The most central 1 cm length of channel from those two points will be the testing region. This approach takes into account that laminar flow takes a certain length to develop and minimizes disturbances in the flow for more accurate results.
Chip Holder Design
- The top and bottom will both be squares.
- The length will be 5.50 cm in order to accommodate the full diameter of the chip.
- There will be 4 screws and 4 nuts, one set at each corner of the square.
- The top holder will have 8 holes for ports and 4 additional holes for the fastening system. The port holes will be directly above and concentric to the port locations.
- The bottom holder will have 4 holes for the fastening system.
Design Considerations
- The design will be chosen by unanimous decision.
- The design chosen must meet all requirements specified in the problem statement.
- The design chosen must allow efficient execution of the experiment.
- The design chosen must have a creative approach.
Treatment Variable Considerations
- The channel will be varied (smooth vs patterned).
- Ionic strength of incubation media will be varied.
- pH of incubation media will be varied.
- Effect of using certain drugs/media/proteins?
Brainstorming Drawing
