Equipment to be Utilized:
- Compound Microscope with camera setup & Slides
- P&G Water Purification Packets
- Sawyer Mini Water Filter System
- MSR Miniworks EX Microfilter System
- Beakers, Pipets, and other experiment materials
- 1 A gallon of distilled water.
A sample of water from a natural source will be obtained using a five-gallon bucket. This will serve as the base sample that all tests will be derived from. From this base sample, five microscope slides will be prepared. After stirring the base sample before each slide to equally distribute any contaminants, a pipet will draw a small amount of the sample to prepare a slide. Each slide will be observed under the microscope and contaminants will be counted. The average of these slides will be used to extrapolate the concentration of contamination of the base water sample.
After stirring the base container, a sample of water roughly one liter will be drawn from the base sample into a sterilized container. This will be repeated three additional times to obtain a total of four one-liter samples. Each sample will be labeled with the method of filtration it will undergo. Prior to filtration, the one-liter samples will be shaken to ensure that any contaminants are evenly distributed. The two physical filter systems (excluding the powder treatment which has a different procedure) will be flushed with distilled water prior to filtering the samples. The powder treatment will be repeated twice, one sample will be filtered with a paper coffee filter as it is transferred to the sterile bottle, the other sample will remove the purified water by pouring it into the sterile bottle carefully so as not to pour any coagulated solid contaminants into the sterile bottle. Each one-liter sample will be treated or filtered according to each manufacturer’s instructions. The filtered water will be filtered directly into a sterilized one-liter bottle or the clean water will be decanted into the sterile bottle in the case of the powder treatment as described previously.
Each one-liter sample will be shaken, and a pipet will be used to create a microscope slide. The slide will be observed under a compound microscope and visible contaminants will be counted. A photo of each microscope slide will be captured. This will be repeated a total of 5 times for each one-liter sample. The average value will be obtained for all 5 trials. The results will be compared to determine how effective each treatment is at purifying the water sample. Data and images will be compiled into an excel sheet and graphically analyzed.