The Campus Microscopy & Imaging Facility (CMIF) at the Ohio State University has recently acquired a new high resolution microscope that uses the latest in high resolution fluorescence techniques. The new system is from Nikon, called the N-SIM S. It includes integrated components to perform super-resolution structured illumination microscopy (SIM) and stochastic optical reconstruction microscopy (STORM). This post highlights four examples of what the system can do and briefly describes the basic principles behind the underlying microscopy techniques.
State of the Art
MIPAR: an easy to use solution to your automated image analysis needs
The image analysis software from MIPAR is a relatively new image analysis program. I have used ImageJ (Fiji) for about a decade now, but I find MIPAR to be much easier to use in terms of implementing and developing image analysis protocols and fully automated solutions.
Light Sheet Microscopy: acquire 3D quantitative images of whole organs with cellular resolution
Light sheet microscopy is a powerful microscopy technique that is ideal for imaging large specimens with cellular resolution quickly, gently, and over long periods of time. This article describes the basics of light sheet microscopy and briefly presents 3 examples looking at what light sheet microscopy can do with whole mouse organs.
The 3i Cleared Tissue LightSheet Microscope
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