In my STEP Signature Project, I focused on investigating a biomarker behind sudden unexpected death in epilepsy in mice models through the Gu Lab. This biomarker was brainstem suppression, and I focused on investigating this through utilizing fiber photometry, which is a form of calcium imaging that allows researchers to study small groups and collections of neurons. In our case, we studied serotonergic neurons which are implicated in the hypothesized molecular pathway of SUDEP.
The biggest impact of my STEP project on myself came in the form of confidence. I grew to be increasingly sure of myself and step out of my comfort zone. I also picked up key laboratory skills and fundamental neuroscience knowledge. This project enabled me to take a field I knew almost nothing about, and turn it into a project that will serve as the basis for change. By taking on this project, I grew from wanting to be a researcher to actively becoming one, which enables me to think critically about experiments and experimental design. Now, I look at the world as an opportunity to research, and I can approach problems easily to form the next solution.
The specific events that led to this contribution include building the machine, experimental design activities, and python data analysis. To start, my first time in lab, I was tasked with ordering and building the fiber photometry machine. Starting out, I had never even heard of fiber photometry, so the idea of now building a multi-thousand dollar machine was terrifying. I studied every video I could, purchased everything needed for the machine, and learned each part in preparation. Still, regardless of how much I studied for this, I ran into complications with understanding the dichromic mirror, which is a small part of the machine that helps direct light to the mouse model. This component was absolutely vital. Without it, I would not be able to take my measurements. At this point in the process is where I got comfortable asking for help when needed. If there is an issue, and I cannot solve it on my own, that does not mean I have to suffer alone. Of course, I should be knowledgeable in my own project, but it is still okay not to know everything. Sometimes, you need to collaborate with others and ask for help.
Furthermore, I was tasked with experimental design of the project. This step was something that was not originally tasked to me, but now I had felt more comfortable asking questions and wanted to try proposing an idea. I had noticed that it would be important to set up a preliminary trial for our machine to ensure we had everything working properly prior to inducing sudden unexpected death in epilepsy in mouse models so we could avoid unnecessary harm of repeated animals. This test run would also help create specific understanding of our controlled variables. I worked up the nerve to speak with my PI about this, and my idea became a reality. Through this, I gained complete control over the experimental design of my project. Watching my planned experiments run smoothly absolutely helped boost my confidence in my research and myself, and I look forward to applying this towards my graduate student project.
Lastly, data analysis. The bane of my project, which I spent over 7 hours working on what I thought would be a setup of the data analysis system, but instead turned out to be myself continually changing the file name. Data analysis was certainly not my strong suit, as my previous lab experiment sent data analysis to another division. However, this proved a necessary challenge. I taught myself python for experimental analysis, I found resources and communities where I could grow with my experiments, I made connections and learned to communicate scientifically with others, and lastly, I finished the data analysis for our recordings. Although our findings proved nothing, they ended up being the first figures I had naturally produced from a lab. A negative result, though not expected, carried so much fulfillment and purpose with it. Research is a slow process and there was still much work to be done, but having the ability to create a figure for a poster or a paper was incredibly rewarding at the time.
My completion of this project and my contributions to it enable me to approach other research opportunities with the knowledge that even if I am unfamiliar with the techniques or processes, I can learn and contribute to the lab environment. This project also gave me the independence and readiness as a researcher to apply to graduate school. From there, I have been accepted at OSU and am now completing my first rotation. Not only am I more confident as a researcher, but I am also more confident as a person. I look forward to continuing to impact the world of research and showing off what I know I can become capable of.










