PEER-REVIEWED PUBLICATIONS

General Articles

• H. Singh, 2019. How to hire an international postdoc for a US position? Mole Cule Advice.  Biophysical Society Bulletin. 16-17.
• H. Singh, 2020. BKCa Channels. Encyclopedia. https://encyclopedia.pub/2596
• H. Singh, 2021. How COVID-19 Has Impacted My Personal Life. Biophysical Society Bulletin. 14.
• Profiles in Biophysics Dec 2022.
• H. Singh, 2023. Self-Management in the Scientific World. Biophysical Society Bulletin. 16.

Peer-reviewed manuscripts

• Sanghvi, S*., Sridharan, D*., Evans, P., Dougherty, J., Szteyn, K., Gabrilovich, D., Dyta, M., Weist, J., Pierre, S. V., Gururaja Rao, S., Halm, D. R., Chen, T., Athanasopoulos, P. S., Dolga, A. M., Yu, L., and Khan, M#., and H. Singh#. 2025. Functional large-conductance calcium and voltage-gated potassium channels in extracellular vesicles act as gatekeepers of structural and functional integrity. Nature Communications. 16, 42.
• Baliga, R. R., Singh, H. and J. Narula. 2025. Sodium or Potassium, and Chloride or Bicarbonate: Which Combination is Worth Its Salt? Eur J Prev Cardiol. 1-7.
• Singh, H., and S. Sanghvi. 2024. Top stories on intracellular potassium channels in cardiac arrhythmia. Heart Rhythm. 21,  10, p2074-2075.
• Rai A. K., Sanghvi, S., Muthukumaran, N. S., Chandrasekera, D., Kadam, A., Kyriazis, I. D., Tomar, D., Ponnalagu, D., Shettigar, V., Khan, M., Singh, H., Goukassian, D., Katare, R., and V. N. S. Garikipati. 2024. Role of mitochondrial ribosomal protein L7/L12 (MRPL12) in diabetic ischemic heart disease.  Free Radical Biology and Medicine. 222 (17).
• Sridharan, D., Dougherty, J. A., Ahmed, U., Sanghvi S. K., Alvi, S. A., Park, K. H., Islam, H., Knoblaugh, S. E., Singh, H., Kirby, E. D., and M. Khan. 2024. Bioorthogonal non-canonical amino acid tagging to track transplanted human induced pluripotent stem cell-specific proteome. Stem Cell Research & Therapy. 186.
• Raut, S. K., Singh, K., Sanghvi, S., Loyo-Celis, V., Varghese, L., Singh, E. R., Gururaja Rao, S., and H. Singh. 2024. Chloride ions in health and disease. Bioscience Reports. 44, BSR20240029. Invited Review
• Gururaja Rao S., Lam, A., Seeley, S. Park, J., Aruva, J., and H. Singh. 2024. The BKCa (Slo) channel regulates the cardiac function of Drosophila. Physiological Reports. 12, e15996.
• Loyo-Celis, V., Patel, D., Sanghvi, S., Kaur, K., Ponnalagu, D., Zheng, Y., Bindra, S., Bhachu, H,. Deschenes, I., Gururaja Rao, S., and H. Singh. 2023. Biophysical Characterization of Chloride Intracellular Channel 6 (CLIC6). Journal of Biological Chemistry. 299(11) 105349. 
• Zapata, R. C., Zhang, D., Yoon, D., Chanond A. Nasamran, C. A., Chilin-Fuentes, D. R., Libster, A., Chaudry, B. S., Lopez-Valencia, M., Ponnalagu, D., Singh, H., Petrascheck, M., and O Osborn. 2023. Targeting Clic1 for the Treatment of Obesity: A Novel Therapeutic Strategy to Reduce Food Intake and Body Weight. Molecular Metabolism. 101794.
• Dall R. D., Cheung M. M., Shweokis P. A., Altasan A., Volpe S. L., Amori, R., Singh, H. and D. Sukumar. 2023. Combined Vitamin D and Magnesium Supplementation Does Not Influence Bone Turnover Markers and Glycemic Indices: A Randomized Controlled Clinical Trial.  Nutrition Research. 33-43.
• Ponnalagu, D., Hamilton, S., Sanghvi, S., Antelo, D., Schwieterman, N., Hansra, I., Xu, X., Gao, E., Edwards, J. C., Bansal, S., Wold, L., Terentyev, D., Janssen, P., Hund, T. J., Khan, M, Kohut, A. R., Koch, W. J., and H. Singh. 2022. CLIC4 Localizes to Mitochondrial Associated Membranes and Mediates Cardioprotection. Science Advances. 8(42) eabo1244.
• Baseeruddi, A. S., Sridharan, D., Shalaan, M., Sanghvi, S., Mergaye, M., Ahmed, U., Mikula, S. K., Singh, H., and M. Khan. 2022. Modulation of mitochondrial bioenergetics by polydopamine nanoparticles in human iPSC-derived cardiomyocytes. ACS Applied Materials & Interfaces. 14, 48, 53451–53461.
• Sanghvi S.,  Szteyn K.,  Ponnalagu D., Sridharan D.,  Lam A.,  Hansra I., Chaudhury A.., Majumdar U., Kohut A., Gururaja Rao S., Khan M., Garg V., and H. Singh. 2022. Inhibition of BKCa channels protects neonatal hearts against myocardial ischemia and reperfusion injury. Cell Death Dis. 8,175.
• Ponnalagu D., Sanghvi S., Bansal S S., and H. Singh. 2022. Ion Channels in Immune Cells: Physiological and Pathophysiological Roles. Immune Cells, Inflammation, and Cardiovascular Diseases. Methods in Signal Transduction. 215-239.
• Yang M., Fu J., Zou J., Sridharan D., Zhao M., Singh H., Krigman J., Khan M., Xin G, and N. Sun. 2022. Assessment of mitophagy in human iPSC-derived cardiomyocytes. Autophagy. 18:2481-2494.
• Cheung M. M., DeLuccia R., Shewakis P. A., Altasan A., Volpe S. L., Amori R., Singh H., and d. Sukumar. 2022. Combined Magnesium and Vitamin D Supplementation on Vitamin D Status, Systemic Inflammation and Blood Pressure: A Randomized Double-Blinded Controlled Trial. Nutrition. 99-100:111674.
• H. Singh. 2021. Mitochondrial Ion Channels in Cardiac Function. American Journal of Physiology-Cell Physiology. 321(5): C812-C825.
• Sengupta S.,  West K. O., Sanghvi S., Laliotis G., Agosto L. M.,  Lynch K. W., Tsichlis P., Singh H., Patrick K. L., M. Guerau-de-Arellano. 2021. PRMT5 promotes symmetric dimethylation of RNA processing proteins and modulates activated T cell alternative splicing and Ca2+/NFAT signaling. ImmunoHorizons.5 (10) 884-897.
• Karekar P., Jansen H. N., Russart K. L. G., Ponnalagu D., Seeley S., Sanghvi S., Smith S. A., Pyter L. M. Singh H♣., S. Guraraja Rao♣. 2021. Tumor-Induced Cardiac Dysfunction: A Potential Role of ROS. Antioxidants. 10(8), 1299.
  ♣ Co-corresponding authors.
  Highlighted by over 100 media outlets including Eureka Alert.
• Galecka S., Kulawiak B., Bednarczyk P., Singh H. and A. Szewczyk. 2021. Single channel properties of mitochondrial large conductance potassium channel formed by BK-VEDEC splice variant. Scientific Reports. 11(1):10925. 
• Nolan K. E., Baer L A., Karekar P., Nelson A. M.,  Stanford K., Doolittle L., Rosas L., Hickman-Davis J.,  Singh H., Ian C. Davis. 2021. Metabolic Shifts Modulate Lung Injury Caused by Infection with H1N1 Influenza A Virus. Virology. 559.111-119. Highlighted in Physicians Weekly.
• Chaudhary A., Wanek, A., Ponnalagu D., Singh. H. and A. Kohut. 2021. Using Speckle Tracking Echocardiography to Detect Induced Regional Strain Changes in the Murine Myocardium by Acoustic Radiation Force. Journal of Cardiovascular Imaging. Apr;29(2):e21
  • Editorial Commentary:  Sang-Hoon Seol. High-Intensity Focused Ultrasound Induced Changes in Left Ventricular Two-Dimensional Speckle-Tracking Strain in a Mouse Model. J Cardiovasc Imaging. 2021;29:e25
• Sahni M., Yeboah B., Das P., Shah D., Ponnalagu D., Singh H., Nelin L., and V. Bhandari. 2020. Novel Biomarkers of bronchopulmonary dysplasia and bronchopulmonary dysplasia-associated pulmonary hypertension. Journal of Perinatology. 40(11):1634-1643.
• Szteyn K., and H. Singh. 2020. BKCa channels as targets for cardioprotection. Antioxidants. 9(8), 760.
• Tombo N., Aliagan A. I., Feng Y., Singh H., and J. Bopassa. 2020. Cardiac Ischemia/Reperfusion Stress Reduces Inner Mitochondrial Membrane Protein (Mitofilin) Levels During Early Reperfusion. Free Radical Biology and Medicine. 158. 181-194. 
• Gardiner B., Dougherty J. A., Ponnalagu D., Singh H., Angelos M., Chen C., and M. Khan. 2020. Measurement of oxidative stress markers in vitro using commercially available kits. Measuring Oxidants and Oxidative Stress in Biological Systems (Springer publishers). 34:39-62.
• Kim J. Y., Bai Y., Jayne L. A., Hector R. D., Persaud A. K., Ong S. S., Rojesh S., Raj R., Feng M. J. H. H., Chung S., Cianciolo R. E., Christman J. W., Campbell M. J., Garder D. S., Baker S. D., Sparreboom A., Govindarajan R., Singh H., Chen T., Poi M., Susztak K., Cobb S. R., and N. S. Pabla. 2020. A Kinome-wide screen identifies a CDKL5-SOX9 regulatory axis in epithelial cell death and kidney injury. Nature Communications. 1924.
• Ponnalagu D.,  and H. Singh. 2020. Insights into the role of mitochondrial ion channels in inflammatory response. Frontiers in Physiology. 11.258.
• Dougherty J.A., Patel N., Kumar N., Gururaja Rao S., Angelos M. G., Singh H., Cai C., and M. Khan. 2020. Human cardiac progenitor cells enhance exosome release and promote angiogenesis under physoxia. Frontiers in Cell and Developmental Biology. 8:130. 
• Gururaja Rao S., Patel N., and H. Singh. 2020. Intracellular Chloride Intracellular Channels: Biomarkers and Therapeutic Targets. Frontiers in Physiology. 11:96. 
• Ponnalagu D., Hussain A.T., Thanawala R., Feng Y., Bopassa J. C., and H. Singh. 2019. Chloride channel blocker IAA-94 increases myocardial infarction by reducing calcium retention capacity of the cardiac mitochondria. Life Sciences. 235:116841.
• Gururaja Rao S., Ponnalagu D., Shah K., Tanda S., Berryman M., Wallace D., and H. Singh. 2019. BKCa (Slo) Channel Regulates Mitochondrial Function and Lifespan in Drosophila melanogaster. Cells. 8(9). pii: E945.
• Covarrubias R., Ismahil M. A., Rokosh G., Hamid T., Accornero F., Singh H., Gumina R. J., Prabhu S., and S. Bansal. 2019. Optimized Protocols for Isolation, Fixation and Flow Cytometric Characterization of Leukocytes in Ischemic Hearts. American Journal of Physiology-Heart and Circulatory Physiology. 317(3) H658-H666.
• Leary S., Das P., Ponnalagu D., Singh. H., and V. Bhandari. 2019. Genetic strain and sex differences in a hyperoxia-induced mouse model of varying severity of bronchopulmonary dysplasia. American Journal of Pathology. 189 (5), 999-1014.
• Goswami S. K., Ponnalagu D., Hussain A. T., Shah K., Karekar P., Gururaja Rao S., Meredith A. L., Khan M., and H. Singh. 2019. Expression and Activation of BKCa Channels in Mice Protects Against Ischemia-Reperfusion Injury of Isolated Hearts by Modulating Mitochondrial Function. Frontiers in Cardiovascular Medicine. 5: 194.
• Shah D., Alam M. A., Singh H., and V. Bhandari. 2018. MicroRNA-34a promotes endothelial dysfunction and mitochondrial-mediated apoptosis in murine models of acute lung injury. American Journal of Respiratory Cell and Molecular Biology. 60 (4), 465-477.
• Chaubey S., Thueson S., Ponnalagu D., Alam M. A., Gheorghe C. P., Aghai Z., Singh H, and V. Bhandari. 2018. Early gestational mesenchymal stem cell secretome attenuates experimental bronchopulmonary dysplasia in part via exosome-associated factor TSG-6. Stem cell research & therapy. 9 (1), 173.
• Sikder K, Shukla S. K., Patel N., Singh H., and K. Rafiq. 2018. High Fat Diet Upregulates Fatty Acid Oxidation and Ketogenesis via Intervention of PPAR-γ. Cellular Physiology and Biochemistry. 48 (3), 1317-1331.
• Lam A., Karekar P., Shah K., Singh H.,* and S. Gururaja Rao. 2018. Drosophila Voltage-Gated Calcium Channel α1-Subunits Regulate Cardiac Function in the Aging Heart. Scientific Reports. 8 (1), 6910 *Co-corresponding authors.
• Patel N.H., Johannesen J., Shah K., Goswami S.K., Patel N.J., Ponnalagu D., Kohut A.R., and H. Singh. 2018. Inhibition of BKCa negatively alters cardiovascular function. Physiological reports. 6(12):e13748.
• Gururaja Rao S., Ponnalagu D., Patel N. J., and H. Singh. 2017. Three decades of Chloride Intracellular Channels: Physiological Roles. Current Protocols in Pharmacology (Wiley Publishers).
• Ponnalagu, D. andH. Singh. 2017. Anion Channels of Mitochondria. Pharmacology of mitochondria. Handbook Exp. Pharmacol. Springer publisher. 240:71-101
• Gururaja Rao S., Ponnalagu D., Singh H., Jin D. J., and H. Singh.  2017. Identification and Characterization of a Bacterial Homolog of Chloride Intracellular Channel (CLIC) Protein Family. Scientific Reports 7(1):8500.
• Kohut A., Patel N., and H. Singh. 2016. Comprehensive echocardiographic assessment of the right ventricle in murine models. Journal of Cardiovascular Ultrasound. 24(3):229-238.
  • Editorial commentary: Kyung-Hee Kim. 2016. Journal of Cardiovascular Ultrasound. 24(3): 199-200.
• Lee A., Lin A., Shah K., Singh H., Miller V., and S. Gururaja Rao. 2016. Optimization of Non-Thermal Plasma Treatment in an in vivo Model Organism. PLoS One. e0160676.
• Ribas V, Drew BG, Zhou Z, Phun J, Kalajian NY, Soleymani T, Daraei P, Widjaja K, Wanagat J, de Aguiar Vallim TQ, Fluitt AH, Bensinger S, Le T, Radu C, Whitelegge JP, Beaven SW, Tontonoz P, Lusis AJ, Parks BW, Vergnes L, Reue K, Singh H., Bopassa JC, Toro L, Stefani E, Watt MJ, Schenk S, Akerstrom T, Kelly M, Pedersen BK, Hewitt SC, Korach KS, and A.L Hevener. 2016. Skeletal muscle ERa action is critical for the maintenance of mitochondrial function and metabolic homeostasis in females. Science Translational Medicine. 8(334):334ra54.
• Ponnalagu D., Gururaja Rao S., Farber J., Xin W., Hussain A.T., Shah K., Tanda S., Edwards J. C., Berryman M., and H Singh. 2016. Data supporting the specificity of CLIC1, CLIC4, CLIC5 and DmCLIC antibodies and their localization in endoplasmic reticulum of cardiomyocytes. Data in Brief. 7:1038–1044.
• Ponnalagu D., Gururaja Rao S., Farber J., Xin W., Hussain A.T., Shah K., Tanda S., Edwards J. C., Berryman M., Bopassa J.C., and H. Singh, 2016. Molecular Identity of Cardiac Mitochondrial Chloride Intracellular Channels. Mitochondrion 27:6-14.
• #Singh H., Li M., Chen S., Sukur S., Lu R., Caputo A., Meredith A.L., Stefani E., and L. Toro. 2016. MaxiK channel interactome reveals its interaction with GABA transporter 3 and heat shock protein 60 in the mammalian brain. Neuroscience. 317:76-107. #corresponding author
• Osei-Owusu P., Owens E. A., Jie L., Reis J. S., Forrester S. J., Kawai T., Eguchi S., Singh H., K. J. Blumer. 2015. Regulation of Renal Hemodynamics and Function by RGS2. PLoS One. e0132594.
• Kabir M.E., Singh H., Lu R., Olde B., Leeb-Lundberg L.M., and J. C. Bopassa. 2015. G Protein-Coupled Estrogen Receptor 1 Mediates Acute Estrogen-Induced Cardioprotection via MEK/ERK/GSK-3β Pathway after Ischemia/Reperfusion. PLoS One. e0135988.
• Toro L., Li M., Zhang Z., Singh H., Wu Y., and E. Stefani. 2014. MaxiK channel and cell signalling. Pflugers Arch. 466:5; 875-886.
• Singh H., Lu R., Bopassa J. C., Meredith A., Stefani E., and L. Toro. 2013. MitoBK(Ca) is encoded by the Kcnma1 gene, and a splicing sequence defines its mitochondrial location. Proceedings of the National Academy of Sciences U S A. 110:26; 10836-10841.
• Martin L. J., Lau E., Singh H., Vergnes L., Tarling E. J., Mehrabian M., Mungrue I., Xiao S., Shih D., Castellani L., Ping P., Reue K., Stefani E., Drake T.A., Bostrom K., and A. J. Lusis. 2013.  Response to Pomozi et al Research Commentary. Circulation Research. 112:e152-e153.
• #Rodríguez P. F. G., #Wu Y., Singh H., Zhao H., Toro L., and E. Stefani. 2012. Building a Fast Scanning Stimulated Emission Depletion Microscope: A Step by Step Guide. Current Microscopy Contributions to Advances in Science and Technology. 2:791-800. #Equal contribution
• Singh H., Stefani E., and L. Toro. 2012. Intracellular BKCa (iBKCa) channels. The Journal of Physiology. 590:5937-5947.
• Martin L. J., Lau E., Singh H., Vergnes L., Tarling E. J., Mehrabian M., Mungrue I., Xiao S., Shih D., Castellani L., Ping P., Reue K., Stefani E., Drake T.A., Bostrom K., and A. J. Lusis. 2012.  Abcc6 localizes to the Mitochondria-Associated Membrane. Circulation Research. 111:516-520.
• Singh H., Lu R., Rodriguez P. F. G., Wu Y., Bopassa J. C., Stefani E., and L. Toro. 2012. Visualization and quantification of cardiac mitochondrial proteins clusters with STED microscopy. Mitochondrion.12:230-236.
• #Singh H. 2010. Two decades with dimorphic ChLoride Intracellular Channels (CLICs). FEBS Letters. 584, 10:2112-2121. #corresponding author
• Singh H., Warburton S., Vondriska T. M., and B. S. Khakh. 2009. Proteomics to identify proteins interacting with P2X2 ligand-gated cation channels. The Journal of Visualized Experiments. 27.
• #Singh H., Cousin M. A., and R. H. Ashley. 2007. Functional reconstitution of mammalian “chloride intracellular channels” CLIC1, CLIC4 and CLIC5 reveals differential regulation by cytoskeletal actin. FEBS J. 274: 6306-6316. #corresponding author
• Singh H., and R. H. Ashley. 2007. CLIC4 (p64H1) and its putative transmembrane domain form poorly selective, redox-regulated ion channels. Molecular Membrane Biology. 24:41-52
• Singh H., and R. H. Ashley. 2006. Redox regulation of CLIC1 by cysteine residues associated with the putative channel pore. Biophysical Journal. 90:1628-38.