Our research focuses on establishing the molecular identity of organellar ion channels, elucidating their targeting mechanism, and studying their biophysical properties. We focus on how intracellular ion channels regulate cellular physiology by altering mitochondrial properties at the functional level. To understand their role in health and disease, our group is studying their role in cardioprotection from ischemia-reperfusion injury and functional role in the aging heart. The specific projects are:
- Molecular identity and regulation of mitochondrial ion channels
- Ion channels in exosomes
- Regulation of organellar structure and function by intracellular ion channels
- Role of intracellular ion channels in cardioprotection from ischemia-reperfusion injury.
- Role of intracellular ion channels in the regulation of cardiac function and aging.
Selected Publications
- Loyo-Celis, V., Patel, D., Sanghvi, S., Kaur, K., Ponnalagu, D., Zheng, Y., Bindra, S., Bhachu, H,. Deschenes, I., Gururaja Rao, S., and H. Singh. 2023. Biophysical Characterization of Chloride Intracellular Channel 6 (CLIC6). Journal of Biological Chemistry. 299(11) 105349.
- Ponnalagu, D., Hamilton, S., Sanghvi, S., Antelo, D., Schwieterman, N., Hansra, I., Xu, X., Gao, E., Edwards, J. C., Bansal, S., Wold, L., Terentyev, D., Janssen, P., Hund, T. J., Khan, M, Kohut, A. R., Koch, W. J., and H. Singh. 2022. CLIC4 Localizes to Mitochondrial Associated Membranes and Mediates Cardioprotection. Science Advances. 8(42) eabo1244.
- Sanghvi S., Szteyn K., Ponnalagu D., Sridharan D., Lam A., Hansra I., Chaudhury A.., Majumdar U., Kohut A., Gururaja Rao S., Khan M., Garg V., and H. Singh. 2022. Inhibition of BKCa channels protects neonatal hearts against myocardial ischemia and reperfusion injury. Cell Death Dis. 8,175.
- H. Singh. 2021. Mitochondrial Ion Channels in Cardiac Function. American Journal of Physiology-Cell Physiology. 321(5): C812-C825.
- Ponnalagu D., Hussain A.T., Thanawala R., Feng Y., Bopassa J. C., and H. Singh. 2019. Chloride channel blocker IAA-94 increases myocardial infarction by reducing calcium retention capacity of the cardiac mitochondria. Life Sciences. 235:116841.