TO BE EDITED
Dot blot or Slot blot analysis
Weddle J, Chan T, Thompson K, Paxton H, Kelly DJ, Dasch G, et al. Effectiveness of a dot-blot immunoassay of anti-Rickettsia tsutsugamushi antibodies for serologic analysis of scrub typhus. Am J Trop Med Hyg. 1995;53(1): 43–46.
PCR
PCR may have only limited value in monitoring treatment. Circulating rickettsiae can be detected by PCR up to 8 days after initiation of antibiotic treatment (Murai et al, 1995).
qPCR
Loop-mediated Isothermal PCR assay (LAMP)
Paris DH, Blacksell SD, Nawtaisong P, Jenjaroen K, Teeraratkul A, Chierakul W, Wuthiekanun V, Kantipong P, Day NP. 2011. Diagnostic accuracy of a loop-mediated isothermal PCR assay for detection of Orientia tsutsugamushi during acute Scrub Typhus infection. PLoS Negl Trop Dis. 2011 Sep;5(9):e1307.
DNA sequencing of specific genes