MLST Panel 1 consists of a set of seven loci that has been studied by several investigators (Sonthayanon et al. 2010; Phetsouvanh et al. 2015) to compare isolates.
The MLST information for these loci consists of a subsection of each of the genes. The length of the MLST portion of a gene compared to the total length of the gene is given for each locus.
These loci in this MLST set are:
glycerol-3-phosphate dehydrogenase (gpsA)
malate dehydrogenase (Mdh)
ribonucleoside-diphosphate reductase beta subunit (nrdF)
NADH dehydrogenase chain F (nuoF)
pyruvate, phospate dikinase precursor (ppdK)
dihydrolipoamide S-succinyltransferase (sucB)
succinyl-CoA synthase alpha chain (sucD)
Some details about the MLST alleles found in the study of Phetsouvanh et al., 2010 is deposited in the MLSTPub website. The specific subpage home for the sequences from Orientia in MLSTPub is at Orientia tsutsugamushi MLST Databases.
Allele sequences through March 2015 are provided at a subpage, while the MLST allele profiles of 92 MLST types are given at a second subpage.
This set of MLST loci has been examined in 163 isolates (most from Southeast Asia). profiles are given which can be related to the information on the 92 MLST types. It is probable that additional alleles will be uncovered at all of the seven loci in this set, and that consequently, additional MLST types will be defined.
MLST typing for genomes: For each of the three groups of MLST loci, sequences of the complete genes are also available from genome sequences of 39 isolates (see Genome Page). Both the partial sequence used for the MLST analysis and the complete gene sequences can be compared. Information on the levels of genetic variation for each MLST partial or complete locus and the phylogenetic information from the various partial and complete MLST loci will be posted in the future as we complete various analyses.