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Panels (<20 compounds)
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Platform |
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Targets and Features |
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Sample Type |
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acrylamide |
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UHPLC-QqQ MS/MS |
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acrylamide with stable isotope internal standard |
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food |
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anthocyanin degradants |
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UHPLC-QqQ MS/MS |
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phenolic acids, e.g. protocatechuic acid (PCA), microbiome metabolites |
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food, blood |
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anthocyanins |
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UHPLC-QqQ MS/MS and UV-Vis |
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glycosides of cyanidin, malvidin, petunidin, peonidin, pelargonidin, delphinidin |
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food |
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anthocyanins |
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UHPLC-QqQ MS/MS and UV-Vis |
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glycosides of cyanidin, malvidin, petunidin, peonidin, pelargonidin, delphinidin, glucuronides |
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blood, tissue, urine |
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apo-carotenoids |
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UHPLC-QqQ MS/MS |
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eccentric cleavage products of lycopene, beta-carotene |
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blood |
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carotenoids |
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C30 HPLC-UV-Vis |
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beta-carotene, lutein, zeaxanthin, lycopene, cryptoxanthin, astaxanthin, neoxanthin, violaxanthin, phytoene, phytofluene |
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food |
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carotenoids |
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C30 HPLC-UV-Vis or SFC QTOF MS |
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beta-carotene, lutein, zeaxanthin, lycopene, cryptoxanthin, astaxanthin, neoxanthin, violaxanthin, phytoene, phytofluene |
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blood, tissue |
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cholesterol |
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NP HPLC-QqQ MS/MS |
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free cholesterol |
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food or in vitro digest |
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e-cigarette vaping components |
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UHPLC-QqQ MS/MS after derivatization |
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propylene glycol, glycerol |
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urine, blood, bronchoalveolar lavage fluid |
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ellagic acid metabolites |
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UHPLC-QqQ MS/MS |
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urolithin A, B, C, D, dimethyl ellagic acid, methyl UC, microbiome metabolites |
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blood, urine |
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flavanone microbial degradants |
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UHPLC-QqQ MS/MS |
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phenolic acids, microbiome metabolites |
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urine |
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flavanones |
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UHPLC-QqQ MS/MS and UV-Vis |
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glycosides of naringenin, hesperetin |
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food |
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flavones |
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UHPLC-QqQ MS/MS |
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apigenin, luteolin, diosmetin, chrysoeriol |
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blood, urine |
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flavones |
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UHPLC-QqQ MS/MS and UV-Vis |
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glycosides of apigenin, luteolin, diosmetin, chrysoeriol |
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food |
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glucosinolates |
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UHPLC-QqQ MS/MS |
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glucoraphanin, glucoerucin, glucoiberin, sinigrin, glucobrassicin |
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food |
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green tea phenolics |
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UHPLC-QTOF MS/MS |
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catechins, EGC, ECG, EGCG, phenyl valerolactones, microbiome metabolites, sulfated and glucuronidated conjugates |
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foods, blood, mouse liver |
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isoflavones and metabolites |
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UHPLC-QqQ MS/MS |
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daidzein, genistein, glycitein; equol, dihydrodaidzein, ODMA, dihydrogenistein, 6-hydroxy ODMA |
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blood, urine |
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isoflavones |
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UHPLC-QqQ MS/MS and UV-Vis |
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glucosides, malonyl glucosides, and acetyl glucosides of daidzein, genistein, and glycitein. Biochanin A, formononetin |
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food |
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isothiocyanate (ITC) metabolites |
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UHPLC-QqQ MS/MS or QTOF MS |
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glutathionyl, cysteinylglycinyl, cysteinyl, N-acetyl cysteinyl |
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blood, urine |
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linalool and metabolite |
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UHPLC-QqQ MS/MS |
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linalool, linalool glucuronide |
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food, blood, urine |
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microcystin congeners – quantitative |
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UHPLC-QqQ MS/MS |
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MC-RR, MC-YR, MC-LR, MC-LA, MC-LW, MC-LF, MC-dspRR, MC-dspLR |
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cell culture, environmental water samples, mouse liver, fish tissue, soil, vegetables |
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microcystins – total |
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UHPLC-QqQ MS/MS |
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MMPB method |
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environmental water samples, mouse liver, fish tissue |
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microcystin-LR conjugates |
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UHPLC-QqQ MS/MS |
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MC-LR-glutathione, MC-LR-cysteine |
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mouse liver |
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naringenin human metabolites |
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UHPLC-QqQ MS/MS |
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naringenin glucuronides, sulfates |
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blood, urine |
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retinoids |
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UHPLC-QqQ MS/MS |
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retinoic acid, retinyl esters, retinol, retinal |
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blood |
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soy saponins |
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UHPLC-QqQ MS/MS |
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type A, B, C, B DDMP |
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food |
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vitamin D |
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UHPLC-QqQ MS/MS and UV-Vis |
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D2, D3 |
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food |
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vitamin D status |
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UHPLC-QqQ MS/MS |
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25OHD2, 25OHD3 |
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blood |
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Panels (>20 compounds)
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Platform |
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Examples |
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Sample Type |
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black raspberry phytochemicals (~80) |
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UHPLC-QTOF-MS and UV-Vis |
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flavonoids, phenolic acids, ellagitannins, procyanidins, ellagic acid, ellagic acid derivatives |
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food |
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burdock phenolics |
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UHPLC-QTOF MS |
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flavonoids |
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plant leaves |
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microcystin congeners and water-borne toxins -survey |
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UHPLC-QTOF MS/MS |
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search accurate mass library of >100 known microcystins and toxins |
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environmental water samples |
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soy phytochemicals |
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UHPLC-QTOF MS |
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phytosterols, saponins, isoflavones, lipids |
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food |
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strawberry phytochemicals |
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UHPLC-QTOF-MS and UV-Vis |
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flavonoids, phenolic acids, ellagitannins, procyanidins, ellagic acid, ellagic acid derivatives |
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food |
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tomato phytochemicals (>150) |
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UHPLC-QTOF-MS and C30 HPLC UV-Vis |
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glycoalkaloids, carotenoids, phenolic acids, flavonoids, lipids |
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food |
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Lipidomics
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Platform |
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Features |
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Sample Type |
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Targeted lipidomics (Lipidyzer®) |
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Infusion/DMS/QqQ MS/MS |
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13 classes, >1100 lipid species |
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blood, tissues, cell culture, milk, egg |
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Untargeted lipidomics |
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SFC-QTOF MS, UHPLC-QTOF MS |
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neutral, polar, sphingolipids, glycosylated |
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Untargeted Metabolomics
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Steps |
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UHPLC-QTOF MS |
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extraction |
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acquisition |
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feature finding |
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bioinformatics |
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Metabolic Phenotyping
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Examples |
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UHPLC-QqQ MS/MS |
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ellagic acid (urolithins) |
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flavanones |
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isoflavones |
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ITC conjugates |
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Data Analysis
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Representations |
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PCA – principal component analysis |
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PLS-DA – penalized logistic regression and partial least squares discriminant analysis |
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VIP Plot – variable importance in projection plot |
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Pathways, Enrichment, Networks |
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| See prices. |
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