Graduate Students
The Nakanishi lab is looking for highly motivated graduate students. We take multiple approaches, including biochemistry, molecular biology, and structural biology, to understand how microRNAs and Argonaute proteins regulate gene expression whose dysregulation causes neurodevelopmental diseases and cancers. For those interested in our research, please contact Dr. Nakanishi (nakanishi.9@osu.edu)!

Postdoctoral position
Two NIH-funded postdoctoral positions are available to study a new type of small RNAs that make AGO3 an extremely competent slicer. The main goal is to reveal the physiological roles and biosynthesis pathways, in addition to the activation mechanism. Candidates must have a solid publication record in biochemistry, molecular biology, or structural biology with excellent communication skills and a strong passion for scientific discovery. We are looking for two types of postdocs.

  1. Applicants with expertise in biochemistry and/or molecular biology with a strong interest in RNA biology. Previous experience in nucleic acid/protein biochemistry using purified components and cell extracts is highly desirable. Part of the project involves analyses of AGO3-small RNA complexes using RNA-Seq approaches in collaboration with the Singh Lab (https://u.osu.edu/singhlab/). Thus, the candidate must be keen on learning bioinformatics. Some prior understanding of bioinformatics tools and computer programming will be beneficial.
  2. Applicants with expertise in Structural Biology. Previous experience in overproduction and purification of recombinant proteins and in structure determination by X-ray crystallography or Cryo-EM is desirable. Skills in insect cells and mammalian cells are preferred.

Those who have experience in immunology and neurodegenerative diseases are welcome. Exceptional resources are available to support the projects, including four AKTA systems and a high-throughput crystal auto-imaging system in our lab, as well as an X-ray diffractometer in core facility.  We also remotely use beamlines at Argonne National Laboratory and a Krios G3i with a K3 detector at CEMAS. Computation resources are available from Ohio Supercomputer Center. Candidates should submit 1) a one-page cover letter describing the applicant’s motivation and interest in our research, 2) CV, and 3) three references to Dr. Kotaro Nakanishi: nakanishi.9@osu.edu.

Technician position
Eligibility: Bachelor’s degree in Biochemistry, Molecular Biology, or Cell Biology. Ideal applicants will be able to work for a long period (at least 3 years).
Jobs: Cell maintenance, Cloning, Protein production (in mammalian cells, insect cells, and E. coli cells), Protein purification, and Recording the experimental work on the lab notebook.
Candidates should submit 1) a one-page cover letter describing the applicant’s motivation, and 2) CV to Dr. Kotaro Nakanishi: nakanishi.9@osu.edu.