Monitoring SARS-CoV-2 RNA in Wastewater with RT-qPCR and Chip-Based RT-dPCR: Sewershed-Level Trends and Relationships to COVID-19

From December 2020 to June 2022, the Hull Lab provided wastewater surveillance support for the Ohio Coronavirus Wastewater Monitoring Network.

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Workflow

1. Receive samples
2. Concentrate solids using vacuum filtration
3. Extract RNA
4. RT-qPCR
5. RT-dPCR
6. Calculate Copies/L RNA in wastewater
7. Limit of Detection Calculation
8. Correlation Analysis