The purpose of the second chip design was to implement improvement from the first chip design. First, the two outer staging wells were removed to reduce air bubbles in the detection well. Then, the circular check valves were enlarged, and the fish-boned check valve was rounded. The detection well and waste well were kept the same.
Second Chip Design
Second Chip Results
With this chip, the results were more consistent and the graphs created from the data were more parallel.
The following are the documents for the second chip: