The third day of the Diagnostics Short Course was devoted to the diagnosis of plant diseases caused by bacteria. Dr. Sally Miller presented a lecture on groups of bacterial pathogens most commonly infecting plants, and approaches to identify them. The lecture was followed up with a lab for the remainder of the day. Participants gained practical experience in identifying symptoms of bacterial diseases, culturing bacteria on various general and semi-selective media, and conducting biochemical tests such as the gram reaction and the oxidase test, and plant based tests including tobacco hypersensitive reaction (HR) and potato and pepper soft rot assays. For diagnosis of bacterial diseases of tomato, participants are directed to OSU fact sheets detailing diagnostic procedures for bacterial spot, bacterial speck, bacterial canker and pith necrosis.