Some techniques we use in the lab

 In Vivo electrophysiological techniques 

Compound muscle action potential (CMAP), Single motor unit potential (SMUP), Motor unit number estimation (MUNE)

A triceps surae compound muscle action potential amplitude from a neonatal mouse model of spinal muscular atrophy.

 

Repetitive nerve stimulation (RNS)

Repetitive nerve stimulation at 3 Hz demonstrating decrement in a patient with myasthenia gravis.

 

 

Single Fiber Electromyography (SFEMG)

Voluntary SFEMG showing muscle fiber action potential jitter and blocking (indicative of loss of fidelity of NMJ transmission).

Video of SFEMG showing jitter and blocking.

 

In Vivo and In Situ Muscle Contractility

Example of a torque readout following a plantarflexion tetanic contraction (125Hz stimulation rate)

 

Example of loss of tetanic torque in an aged versus young rat indicative of NMJ transmission failure

 

Immunohistochemistry and Confocal Imaging

 

 

 

 

 

 

 

 

 

Neuromuscular junction staining in an aged C57BL/6J mouse.

 

Motoneuronal staining in pig.

 

In Vivo MRI Muscle Assessment

Hind limb imaging in an aged C57BL/6J mouse with region of interest identified to quantify muscle volume.