DATA

Links to data are below.

Due to upload size constraints the files were divided into zipped portions of raw assembly files. Download each file individually and concatenate them via command line.

Transcriptome Shotgun Assembly (TSA) Acrorhagi from a single aggressive polyp of Anthopleura elegantissima: 

1. Aggressive_Clean.part-1
2. Aggressive_Clean.part-2
3. Aggressive_Clean.part-3
4. Aggressive_Clean.part-4
5. Aggressive_Clean.part-5

For each transcriptome, reads were filtered using the program Trimmomatic to remove adapters, leading and trailing low quality bases (using a sliding window greater than 3 bases), reads shorter than 36 bases in length, and reads that fell below an average quality score of 15 using a four base sliding window. The cleaned data were checked using the program FastQC to ensure that low quality reads and regions were removed. Raw data were assembled de novo Trinity using default parameters. Assembled transcripts containing sequencing primers/vectors were removed (46 sequences total).

Transcriptome Shotgun Assembly (TSA) Acrorhagi from non-aggressive polyps of Anthopleura elegantissima: 

1. Ae-AcrorCombined_Trinity_Cleaned.part-1
2. Ae-AcrorCombined_Trinity_Cleaned.part-2
3. Ae-AcrorCombined_Trinity_Cleaned.part-3

For each transcriptome, reads were filtered using the program Trimmomatic to remove adapters, leading and trailing low quality bases (using a sliding window greater than 3 bases), reads shorter than 36 bases in length, and reads that fell below an average quality score of 15 using a four base sliding window. The cleaned data were checked using the program FastQC to ensure that low quality reads and regions were removed. Raw data were assembled de novo Trinity using default parameters. Assembled transcripts containing sequencing primers/vectors were removed (16 sequences total).