Impact of astrocytic network manipulation on K+ homeostasis in health and stroke
“Astrocytes are supportive cells that play a large role in controlling the extracellular space. They are especially active when it comes to clearing K+ ions after a neuronal action potential. This role is absolutely crucial because a high extracellular K+ concentration ([K+]e) can prevent successive action potential firing. Since these K+ ions are charged particles, astrocytes themselves risk becoming depolarized when uptaking large volumes. In order to combat this, they use gap junction coupling with neighboring cells to maintain a healthy membrane potential. Interfering with this network could cause K+ dysfunction, but it has not yet been explored.
We are going to measure [K+]e by making a K+ selective electrode for in vitro data collection. In order to manipulate the astrocytic network, we will use a Gq-Dreadd mouse gene line. This mouse line allows us to potentiate or close off the network at our discretion. OGD will be used to simulate ischemia in vitro.
We are anticipating a faster K+ clearance when the network is potentiated and a slower clearance without the network.”